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Mircury array labeling kit

Manufactured by Qiagen
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Sourced in Denmark

The MiRCURY array labeling kit is a tool designed for the labeling of RNA samples, including microRNA (miRNA), for use in microarray analysis. The kit provides reagents and protocols for the labeling of RNA samples prior to array hybridization. The core function of this product is to facilitate the preparation of labeled RNA samples for microarray-based expression analysis.

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Lab products found in correlation

Mirvana mirna isolation kit, by Thermo Fisher Scientific (8 mentions) Genepix 4000b scanner, by Molecular Devices (8 mentions) Genepix pro 6, by Molecular Devices (7 mentions) Trizol reagent, by Molecular Research Center (4 mentions) Trizol reagent, by Thermo Fisher Scientific (4 mentions) Genepix 4, by Molecular Devices (3 mentions) Mirna microarray chip, by Qiagen (3 mentions) Mircury locked nucleic acid microrna arrays v8.0, by Qiagen (2 mentions) Mircury locked nucleic acid microrna arrays, by Qiagen (2 mentions) Mircury lna microrna array, by Qiagen (2 mentions) Mircury array microarray kit, by Qiagen (2 mentions) Genepix 4000b laser scanner, by Molecular Devices (2 mentions) Avian myeloblastosis virus transcriptase, by Takara Bio (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Genepix 4000b microarray scanner, by Molecular Devices (1 mentions) Genepix 4000b laser, by Molecular Devices (1 mentions)

12 protocols using mircury array labeling kit

1

miRNA Expression Profiling in A549 Cells

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Total RNAs from A549 and A549 cells with TGF-β1 treatment were isolated with Trizol reagent (Invitrogen, Carlsbad, CA, USA), and miRNA fractions were further purified using a mirVana miRNA isolation kit (Ambion, Austin, TX, USA). The isolated miRNAs from two different cell lines were labeled with Hy3 using the miRCURY Array Labeling kit (Exiqon, Vedbaek, Denmark) and hybridized, respectively, on a miRCURY LNA microRNA Array (v 8.0, Exiqon) as was described.57 (link) Microarray images were acquired using a Genepix 4000B scanner (Axon Instruments, Union City, CA, USA), processed and analyzed using Genepix Pro 6.0 software (Axon Instruments).
Shi Z.M., Wang L., Shen H., Jiang C.F., Ge X., Li D.M., Wen Y.Y., Sun H.R., Pan M.H., Li W., Shu Y.Q., Liu L.Z., Peiper S.C., He J, & Jiang B.H. (2017). Downregulation of miR-218 contributes to epithelial–mesenchymal transition and tumor metastasis in lung cancer by targeting Slug/ZEB2 signaling. Oncogene, 36(18), 2577-2588.
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2

Profiling Placental miRNA Expression

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miRNA expression profiles were measured using miRNA microarray analysis. Total RNA was extracted from placental tissues using TRIzol reagent (Molecular Research Center, Inc., Cincinnati, OH, U.S.A.), and the miRNA fraction was further purified by an mirVana miRNA isolation kit (Ambion, Austin, TX) following the manufacturers’ guidelines. The isolated miRNAs were labeled with Hy3 using the miRCURY array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized with miRCURY locked nucleic acid (LNA) microRNA arrays (v8.0; Exiqon). Replicated miRNAs were averaged, and miRNAs with intensities ≥ 50 in all samples were used to calculate a normalization factor. Expressed data were normalized by median normalization. Microarray images were taken with a Genepix 4000B scanner (Axon Instruments, Foster City, CA, U.S.A.) and analyzed with Genepix Pro 6.0 software (Axon Instruments).
Suo M., Sun Y., Yang H., Ji J., He Y., Dong L., Wang Y., Zhang Y., Zhang Y, & Hao M. (2020). miR-183-5p suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting MMP-9 in preeclampsia. Bioscience Reports, 40(6), BSR20192575.
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3

Microarray-based miRNA Expression Profiling

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Total RNA enriched with miRNAs was extracted from liver tissues using the mirVana™ miRNA Isolation kit (Ambion, Austin, TX, USA) and subjected to direct labeling with an Hy3™ fluorescent label using the miRCURY™ Array Labeling kit (Exiqon, Vedbæk, Denmark). Subsequent to labeling, the RNA was concentrated with the RNeasy Mini kit (Qiagen, Hilden, Germany) followed by hybridization using the miRCURY™ Array Microarray kit (Exiqon, Vedbaek, Denmark). The hybridized arrays were scanned with the Genepix 4000B scanner (Axon Instruments, Sunnyvale, CA, USA) and the microarray images were background-subtracted, normalized and subjected to further analysis. Mature miRNAs with comparative expression levels of >2.0 or <0.5 between AFL and C12, or ASH and C16 were considered as dysregulated miRNAs.
To further validate the expression levels of significantly dysregulated miRNAs revealed by microarray, stem-loop qPCR was performed with stem-loop antisense primer mix (Applied Biosystems, Foster City, CA, USA) and Avian Myeloblastosis Virus transcriptase (Takara Bio, Inc., Dalian, China). All PCR primers were designed based on miRNA sequences released by the Sanger Institute (16 (link)). The relative quantity of each miRNA was normalized to U6 RNA and calculated using the following equation: 2−ΔCT, where ΔCT = CTmiRNA-CTU6.
CHEN Y.P., JIN X., KONG M, & LI Y.M. (2014). Pattern of microRNA expression associated with different stages of alcoholic liver disease in rat models. Molecular Medicine Reports, 10(3), 1195-1204.
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4

Profiling miRNA Expression in Carotid Injury

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miRNA microarray analysis was used to compared the miRNA expression profiles between the uninjured carotid arteries from 2 rats of sham group and injured carotid arteries from 2 rats of injury group. Total RNAs were extracted using TRIzol reagent (Molecular Research Center, Inc., Cincinnati, OH, USA), and the miRNA fraction was further purified by a mirVana miRNA isolation kit (Ambion, Austin, TX) according to the manufacturer’s recommendation. The isolated miRNAs were labeled with Hy3 using the miRCURY array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized with miRCURY locked nucleic acid (LNA) microRNA arrays (v8.0; Exiqon). Microarray images were taken with a Genepix 4000B scanner (Axon Instruments, Foster City, CA, USA) and analyzed with Genepix Pro 6.0 software (Axon Instruments).
Huang R., Huang Y., Zeng G., Li M, & Jin Y. (2020). Ursodeoxycholic acid inhibits intimal hyperplasia, vascular smooth muscle cell excessive proliferation, migration via blocking miR-21/PTEN/AKT/mTOR signaling pathway. Cell Cycle, 19(8), 918-932.
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5

Profiling miRNA Expression in Cartilage

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miRNA microarray analysis was performed to identify miRNA expression profiles in human cartilage tissues. Total RNA was isolated using TRIzol reagent (Molecular Research Center, Inc., Cincinnati, OH, U.S.A.), and mirVana miRNA isolation kit (Ambion, Austin, TX) was used to further purify miRNA fraction according to the manufacturer’s instructions. The isolated miRNAs were labeled with Hy3 using the miRCURY array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized with miRCURY locked nucleic acid (LNA) microRNA arrays (v8.0; Exiqon). Replicated miRNAs were averaged, and miRNAs with intensities ≥50 in all samples were used to calculate a normalization factor. Data normalization was performed using the quantiles method. Microarray images were taken with a Genepix 4000B scanner (Axon Instruments, Foster City, CA, U.S.A.) and analyzed with Genepix Pro 6.0 software (Axon Instruments).
An Y., Wan G., Tao J., Cui M., Zhou Q, & Hou W. (2020). Down-regulation of microRNA-203a suppresses IL-1β-induced inflammation and cartilage degradation in human chondrocytes through Smad3 signaling. Bioscience Reports, 40(3), BSR20192723.
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6

miRNA Microarray Analysis of Pathways

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A miRNA microarray analysis was performed to identify the miRNA expression profiles of the extracted RNA samples. Total RNA was isolated by using TRIzol reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and was purified to obtain the miRNA fraction, further purified by using a mirVana miRNA isolation kit (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. The isolated samples were labeled with Hy3 by using the miRCURY array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized with miRCURY-locked nucleic acid (LNA) microRNA arrays (v8.0; Exiqon). After hybridization, microarray images were taken with a Genepix 4000B scanner (Axon Instruments, Foster City, CA, USA) and analyzed with Genepix Pro 6.0 software (Axon Instruments). Data normalization was performed by using the quantiles method. We have selected the TLR4, FOS, FAS, MMP9, and MAPK pathways based on miRNA analysis. The qPCR confirmed these pathways.
Singh R., Chandel S., Ghosh A., Matta T., Gautam A., Bhattacharya A., Babu S.S., Sukla S., Nag D., Ravichandiran V., Roy S, & Ghosh D. (2022). Glucogallin Attenuates the LPS-Induced Signaling in Macrophages and Protects Mice against Sepsis. International Journal of Molecular Sciences, 23(19), 11254.
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7

miRNA Microarray Expression Profiling

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miRNA microarray analysis was conducted to determine miRNA expression profiles in tissue samples. Total RNAs were extracted by TRIzol reagent (Invitrogen, Carlsbad, CA, U.S.A.), and the miRNA fraction was further purified using a mirVana miRNA isolation kit (Ambion, Austin, TX) according to manufacturer’s protocol. The isolated miRNAs were labeled with Hy3 using the miRCURY array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized with miRCURY locked nucleic acid (LNA) microRNA arrays (v8.0; Exiqon). Microarray images were obtained using a Genepix 4000B scanner (Axon Instruments, Foster City, CA, U.S.A.) and analyzed with Genepix Pro 6.0 software (Axon Instruments).
Liao Y., Yin X., Deng Y, & Peng X. (2018). MiR-140-5p suppresses retinoblastoma cell growth via inhibiting c-Met/AKT/mTOR pathway. Bioscience Reports, 38(6), BSR20180776.
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8

Profiling miRNA Expression in Obese Rats

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Total RNA of the CC from obese rats with or without ED was harvested and extracted using Trizol reagent (Invitrogen, San Diego, CA, USA) according to the manufacturer’s instructions. The concentration and purity of RNA were determined by measuring absorbance in a spectrophotometer at 260 and 280 nm. Quantified RNA samples were labeled using the miRCURYTM array labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on the miRCURYTM array microarray kit (Exiqon). After washing, the microarrays were scanned with a GenePix4000B microarray scanner (Axon Instruments, Sunnyvale, CA, USA) and analyzed with Pro 6.0 software (Axon Instruments). Expression data were normalized using median normalization. After normalization, average values of the replicate spots of each miRNA were used for statistical analysis. Differentially expressed miRNAs were identified as a fold change greater than 1.5.
Bai Y., Zhang L., Jiang Y., Ju J., Li G., Xu J., Jiang X., Zhang P., Lang L., Sadkovaya O., Glybochko P.V., Zhang W, & Yang B. (2017). Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction. Sexual Medicine, 5(4), e261-e271.
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9

Profiling miRNA Expression in PBMC

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Microarray expression profiling was performed using 5 µg total RNA extracted from peripheral blood mononuclear cells (PBMCs) collected from 22 cases and 19 controls. MiRCURYTM Array Labeling kit (Exiqon, Vedbaek, Denmark) was utilized to label miRNAs fluorescently, and then miRNA microarray chips (Exiqon) were utilized to hybridize miRNAs. Hybridization data were collected using GenePix 4000B laser scanner (Axon Instruments, Foster City, CA, USA), and GenePix 4.0 software (Axon Instruments) was utilized to digitize and analyze the images.
Wang L., Wu W., Chen J., Li Y., Xu M, & Cai Y. (2019). miR-122 and miR-199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway. International Journal of Molecular Medicine, 43(3), 1373-1381.
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10

Profiling miRNA Expression under Hypoxia

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Total RNA was extracted from MB-231 and MB-468 cells cultured under normoxic or hypoxic conditions with TRIzol reagent (Invitrogen, Carlsbad, CA), and the miRNA fraction was further purified with the mirVanaTM miRNA isolation kit (Ambion, Austin, TX). A miRCURYTM Array Labeling kit (Exiqon, Vedbaek, Denmark) was used to label the isolated miRNA with Hy3, and samples were hybridized on a miRCURYTM LNA microRNA Array (v 8.0, Exiqon). A Genepix 4000B scanner (Axon Instruments, Union City, CA) was used to acquire microarray images, and Genepix Pro 6.0 software (Axon Instruments) and Excel were used for data processing and analysis.
Li H., Su X., Li J., Liu W., Pan G., Mao A., Liu J., Zhang Q., Rao L., Xie X, & Sheng X. (2022). Hypoxia induces docetaxel resistance in triple-negative breast cancer via the HIF-1α/miR-494/Survivin signaling pathway. Neoplasia (New York, N.Y.), 32, 100821.
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