Pyromark gold 96 reagents kit

The RNA stabilization solution (RNAlater), Pyromark PCR kit, and Pyromark Gold 96 Reagents kit were purchased from Qiagen (Hilden, Germany). The Nucleospin RNA II kit was obtained from Macherey-Nagel (Düren, Germany). Superscript II RNase H-RT, primers, and NovexWedgeWell™ 4–20% Tris-Glycine gel were from Invitrogen (Carlsbad, USA), and RNase inhibitor was obtained from AMRESCO (Solon, USA). The Protein Assay Dye Reagent Concentrate Bradford was purchased from BioRad (München, Germany). Polyclonal goat anti-ADIPOR1 (sc-46749), anti-ADIPOR2 (sc-46756), and mouse anti-leptin (sc-48408) antibodies were from Santa Cruz Biotechnology (Dallas, USA). The rabbit polyclonal anti–LEPR (ab104403) antibody was obtained from Abcam (Cambridge, UK). The mouse monoclonal anti-cytokeratin pan-antibody (KL1) was sourced from Dako Cytomation (Glostrup, Denmark). DyNAmo ColorFlash SYBR Green and the SuperSignal West Pico Chemiluminescent Substrate detection kit were obtained from Fisher Scientific (Waltham, USA). The SPlink Broad kit with DAB chromogen was purchased from GBI Labs Golden Bridge International (Bothell, USA). The Wizard DNA Clean-Up system was bought from Promega (Madison, USA).