96 well polystyrene plate

Manufactured by Corning

Sourced in United States

The 96-well polystyrene plates are a commonly used laboratory equipment for various biochemical and cell culture applications. These plates provide a standardized format with 96 individual wells, each designed to hold small volumes of samples or reagents. The polystyrene material is a widely accepted substrate for cell growth and assay development. The plates are durable, disposable, and can be used in a variety of automated and manual laboratory processes.

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Lab products found in correlation

Powerwave xs2 microplate spectrophotometer, by Agilent Technologies (2 mentions) Infinite m200 pro, by Tecan (2 mentions) Spectramax i3 spectrometer, by Molecular Devices (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions) Spectramax m2 microplate reader, by Molecular Devices (1 mentions) Beckman coulterdtx880, by Beckman Coulter (1 mentions) Versamax, by Molecular Devices (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) β glycerophosphate, by Merck Group (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Infinite m1000 pro, by Tecan (1 mentions) Wallac 1420 victor2, by PerkinElmer (1 mentions) Clariostar plate reader, by BMG Labtech (1 mentions) Maxq 4000 shaking incubator, by Thermo Fisher Scientific (1 mentions) α chymotrypsin, by Merck Group (1 mentions) Filtermax f5, by Molecular Devices (1 mentions) Spectramax m5, by Molecular Devices (1 mentions) Acetonitrile, by Avantor (1 mentions) Acetic acid, by Molecular Devices (1 mentions)

Close spelling variants of this product

96-well plates (3030 citations) 96-well polystyrene microtiter plates (23 citations) Polystyrene plates (20 citations) 96 wells (10 citations) 96-well flat-bottom polystyrene plates (9 citations) 96-well round-bottom polystyrene plates (7 citations) High-binding polystyrene 96-well plates (7 citations) Costar Polystyrene High Binding 96-well plates (4 citations) Costar white polystyrene opaque 96-well plates (3 citations) 96-well polystyrene-coated tissue culture plates (2 citations)

62 protocols using 96 well polystyrene plate

Endopolyphosphatase Activity Assay

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Certain nudix
hydrolases were examined for endopolyphosphatase activity, typically
in a two-stage assay. In the first stage, polyP-MU or SAP-treated
polyP-NOL was incubated with endoacting enzyme (Nudt2 or Nudt3, Fitzgerald
Industries International; Acton, MA) in the appropriate buffer at
37 °C, after which the reactions were chilled on ice. Buffer
conditions were the following: for Nudt2, 8 mM SAP-treated polyP-NOL
or 2 mM polyP-MU, 50 mM HEPES pH 7.4, and 5 mM MgCl₂; for
Nudt3: 5.5 mM SAP-treated polyP-NOL or 2 mM polyP-MU, 25 mM HEPES
pH 7.4, 20 mM NaCl, 10 mM MgCl₂, and 1 mM dithiothreitol.
For the second stage, a solution of CIAP in 100 mM Tris-HCl pH
8.8, 0.2 mM ZnCl₂ was prepared and warmed to 37 °C
in 96-well polystyrene plates (Corning; Tewksbury, MA). The second
stage was initiated by pipetting 100 μL of the chilled nudix-polyP
reaction into prewarmed wells containing 100 μL CIAP solution,
after which the rate of dye release was monitored spectrophotometrically
at 37 °C. For polyP-NOL substrate, absorbance at 400 nm was measured
using a SpectraMax M2 microplate reader (Molecular Devices; Sunnyvale,
CA); for polyP-MU substrate, fluorescence was quantified in fluorescence
mode using excitation at 360 nm, emission at 450 nm, and a 435 nm
cutoff filter.

Hebbard C.F., Wang Y., Baker C.J, & Morrissey J.H. (2014). Synthesis and Evaluation of Chromogenic and Fluorogenic Substrates for High-Throughput Detection of Enzymes That Hydrolyze Inorganic Polyphosphate. Biomacromolecules, 15(8), 3190-3196.

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Biofilm Formation and Growth Kinetics

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Bacterial strains were cultured at 37°C and growth curves were determined by measuring the optical density (OD) values at 600 nm, at 1 h intervals.
The biofilm formation of the bacteria was detected using a semi-quantitative microtiter plate assay with 96-well polystyrene plates (Corning, USA). Overnight cultures were diluted 1:200 in 200 μl TSB and cultured at 37°C for 6 or 12 h, with or without the addition of 250 ng/ml ATc. The planktonic culture was removed for detection of cell density at OD₆₀₀. The biofilms on the bottom of wells were washed with phosphate-buffered saline (PBS), and fixed with 99% methanol. The fixed biofilms were stained with 2% (wt/vol) crystal violet, resolved with acetic acid (30%), and detected at 570 nm with a spectrophotometer (Beckman Coulter DTX880, Beckman Instruments, USA).

Xu T., Wu Y., Lin Z., Bertram R., Götz F., Zhang Y, & Qu D. (2017). Identification of Genes Controlled by the Essential YycFG Two-Component System Reveals a Role for Biofilm Modulation in Staphylococcus epidermidis. Frontiers in Microbiology, 8, 724.

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Cytotoxicity Assay of α-Synuclein in SH-SY5Y Cells

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SH‐SY5Y cells were harvested and plated in 96‐well polystyrene plates (Corning) at a concentration of 1.5 × 10⁴ cells per 100 μl of medium per well. Plates were incubated at 37°C for 24 hours to allow cells to attach. After 24 hours, the medium was exchanged with 100 μl of the preincubated mixtures of α‐synuclein with CM. The same volume of DMEM was added to the control cultures. Plates were then incubated at 37°C for an additional 24 and 48 hours. Cell viability was measured by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT) reduction assays essentially as described [28]. Briefly, after the cells were incubated with the various medium samples, MTT was added to a final concentration of 0.5 mg/ml. After incubation at 37°C for 3 hours, the plates were centrifuged, and the medium was aspirated from each well. The absorbance was measured by an enzyme‐linked immunosorbent assay (ELISA) microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, http://www.moleculardevices.com) at 490 nm. Cell viability was calculated by dividing the absorbance of wells containing samples (corrected for background) by the absorbance of wells containing medium alone (corrected for background).

Oh S.H., Kim H.N., Park H.J., Shin J.Y., Kim D.Y, & Lee P.H. (2016). The Cleavage Effect of Mesenchymal Stem Cell and Its Derived Matrix Metalloproteinase‐2 on Extracellular α‐Synuclein Aggregates in Parkinsonian Models. Stem Cells Translational Medicine, 6(3), 949-961.

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MC3T3-E1 Osteoblast Differentiation Protocol

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Mouse pre-osteoblastic cell line MC3T3-E1 subclone 14 - RRID:CVCL_0409 (American Type Culture Collection, VA) were cultivated in alpha-minimum essential medium (Alfa-MEM; Gibco/Thermo Fisher Scientific, Waltham, MA, USA), 10 % fetal bovine serum (FBS; Gibco/Thermo Fisher Scientific), 1% antibiotic-antimycotic (Gibco/Thermo Fisher Scientific). The cells were maintained in a humidified environment at 37°C with 5% CO₂ and 95% atmospheric air. At subconfluence cells were subcultured and plated in 96-well polystyrene plates (Corning Inc., Corning, NY, USA) at cell density of 10 000 cells/well with osteogenic medium, composed of expansion medium supplemented with 7 mM β-glycerophosphate (Sigma-Aldrich, St. Louis, MO, USA) and 50 μg/mL ascorbic acid (Sigma-Aldrich) for 24 h before exposure to the extracts of the cements.

Abrão S.M., Gregorio D., Azevedo M.K., Mori G.G., Poli-Frederico R.C, & Maia L.P. (2023). Cytotoxicity and genotoxicity of Bio-C Repair, Endosequence BC Root Repair, MTA Angelus and MTA Repair HP. Brazilian Dental Journal, 34(2), 14-20.

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Cell Viability Assay with Inhibitors

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Each cell line was seeded in 96-well polystyrene plates (Corning Incorporated, New York, NY, USA) at a density of 1 × 10³ cells per well in each supplemented culture medium with 1 μM dutasteride, anastrozole and ASP9521. The assay was carried out in duplicate and untreated cells were used as a control. Cells were cultured in a humidified 5% CO₂ atmosphere at 37 °C for 24 h. Then, the viability assay was performed by adding MTT and measuring the absorbance at 568 nm. The data were expressed as a percentage of cell viability with respect to the control cells.

Crespo B., Illera J.C., Silvan G., Lopez-Plaza P., Herrera de la Muela M., de la Puente Yagüe M., Diaz del Arco C., Illera M.J, & Caceres S. (2024). Androgen and Estrogen β Receptor Expression Enhances Efficacy of Antihormonal Treatments in Triple-Negative Breast Cancer Cell Lines. International Journal of Molecular Sciences, 25(3), 1471.

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Enzyme Deactivation Assay for Antidiabetic Evaluation

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The enzyme deactivation assay was carried out following the modified microplates method adapted from Ning et al. [72 (link)]. A volume of 20 µL of the plant extract in different concentrations or acarbose solution (31.25 to 1000 µg/mL), isolates or acarbose solution (31.25 to 1000 µM), or solvent control was mixed with α-glucosidase (40 µL, 0.075 U/mL in potassium phosphate buffer solution (100 mM, pH 6.8) in 96-well polystyrene plates (Corning, New York City, NY, USA) and then incubated for 15 min at 37 °C. Afterwards, p-NPG solution in the buffer solution (40 µL, 1 mM) was added to the mixture, followed by further incubation for 30 min at 37 °C. The reaction was terminated by adding Na₂CO₃ solution (100 μL, 200 mM) to the wells. The spectrophotometric observation was then conducted to determine the absorbance of p-nitrophenol released from the reaction under 405 nm wavelength in a microplate reader (Tecan Infinite M1000 PRO, Männedorf, Switzerland). The percentage of inhibition was calculated from the following formula:

Percentage of inhibition (%) = \frac{A_{c} - A_{s}}{A_{c}} \times 100 %

where A_c is the absorbance of the solvent control and enzymatic reaction system and A_s is the absorbance of the sample with the enzymatic reaction system. The inhibitory activity was expressed in the value of half minimal inhibitory concentration (IC₅₀).

Asmara A.P., Prasansuklab A., Tencomnao T, & Ung A.T. (2023). Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia. Molecules, 28(3), 1028.

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GBA Activity Measurement in DBS Samples

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GBA activity was measured by modifying the method proposed by Chamoles et al.^{15 (link)} One 3 mm diameter blood spot (approximately 3.2 μl) from each DBS sample was punched into 96-well polystyrene plates (Corning, Corning, NY, USA) using a DBS Puncher. Each plate contained at least two blank filter papers. We prepared 0.2 mol l⁻¹ citrate-phosphate buffer (pH 5.2, containing 0.9% taurodeoxycholate and 0.15% Triton-X 100) as elution buffer. Fifty microliters of 5 mmol l⁻¹ 4-methyl-umbelliferyl-β-d-glucopyranoside distilled in the elution buffer as a substrate was added to each well. Plates were sealed with an adhesive film (Corning) and incubated (400 r.p.m. shaking, 37 °C, 20 h; Labsystems iEMS, Finland). The seal was removed and the reaction was stopped with 200 μl glycine-carbonate buffer (0.17 mol l⁻¹, pH 10.5). Samples (200 μl) were transferred to a new 96-well polystyrene plate to avoid interference of the filter paper with fluorescence. Fluorescence was detected in a Wallac 1420 victor^{2 (link)} microplate reader (excitation 355 nm, emission 460 nm; Perkin Elmer, Waltham, MA, USA). After correcting readings with blanks, we used the 4-MU calibration curve to convert fluorescence readings into moles of 4-MU. Enzymatic activity was expressed as micromoles of 4-MU hydrolyzed substrate per liter of blood per hour. Reagents used above were obtained from Sigma (St Louis, MO, USA).

Kang L., Zhan X., Gu X, & Zhang H. (2017). Successful newborn screening for Gaucher disease using fluorometric assay in China. Journal of Human Genetics, 62(8), 763-768.

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Culturing Roseobacter sp. AzwK-3b

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Roseobacter sp. AzwK-3b was obtained from Colleen Hansel (Woods Hole Oceanographic Institution, Falmouth, MA), who isolated the strain (20 (link)). Cultures were grown in a defined medium, which was established by modifying the predefined artificial seawater (ASW) medium (35 (link)). This modified artificial seawater medium is referred to here as ASW_m, and its composition is shown in Table 1. ASW_m contained sodium acetate as the sole carbon source (at concentrations specified per experiment), 200 mM sodium chloride (instead of 428 mM, as in ASW), ammonium as the nitrogen source (instead of nitrate, as in ASW), and five vitamins that were added as a supplement. In manganese-supplemented ASW_m, manganese chloride (MnCl₂) was added to a final concentration of 200 μM. Cultures were grown at 30°C in appropriate (100-ml) Erlenmeyer flasks (shaking at 150 strokes per minute) or 96-well polystyrene plates (Corning, Inc.) closed with a lid and Parafilm (shaking at 300 strokes per minute). For flask cultures, a MaxQ 4000 shaking incubator (Thermo Fisher Scientific) was used. Plates were incubated in a CLARIOstar plate reader (BMG Labtech), and absorbance measurements were done at 600 nm (A₆₀₀) and with path length correction, so as to present absorbance per 1 cm.

Zerfaß C., Christie-Oleza J.A, & Soyer O.S. (2019). Manganese Oxide Biomineralization Provides Protection against Nitrite Toxicity in a Cell-Density-Dependent Manner. Applied and Environmental Microbiology, 85(2), e02129-18.

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Fluorometric Assay for U-Omp19 Protease Inhibition

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U-Omp19 protease inhibitor activity was measured as the reduction in the rates of α-Chymotrypsin mediated cleavage of specific fluorogenic substrate, Suc-Ala-Ala-Pro-Phe-AMC, (Calbiochem, California, USA) as previously described.¹⁰ (link) Briefly, α-Chymotrypsin (1.5 nM, Sigma-Aldrich) was incubated in the presence or absence of U-Omp19 (50 μM) for 1 h at RT in black, half-area, flat-bottom, 96-well polystyrene plates (Corning Inc., New York, NY, USA). Subsequently, 60 μM substrate solution was added to each well and afterwards substrate fluorescence emission was measured kinetically with a fluorometer (FilterMax F5, Molecular Devices, USA) using the 360 nm and 465 nm excitation and emission filters, respectively.

Darriba M.L., Cerutti M.L., Bruno L., Cassataro J, & Pasquevich K.A. (2021). Stability Studies of the Vaccine Adjuvant U-Omp19. Journal of Pharmaceutical Sciences, 110(2), 707-718.

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Quantification of Peptide Conjugates

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A total of 1 ml of a 1:1 mixture of acetonitrile (VWR Chemicals, Radnor, PA) and water was added to each well of the 96-well plates containing the dried peptide conjugates. The plates were treated in an ultrasound bath for 20 min at 40 °C; insoluble matter was removed by centrifugation (2800×g, 5 min) and 2 × 180 µl from each well were transferred to two 96-well polystyrene plates (Corning, Amsterdam, Netherlands). In one plate, 20 µl of acetic acid (Roth, Karlsruhe, Germany) were added to each well, the OD₄₉₀ was measured in a plate reader (SpectraMax M5, Molecular Devices, Sunnyvale, CA), and the concentrations of the peptide solutions were determined according to a calibration curve using FAM in 45% acetonitrile/45% water/10% acetic acid as standard. Then, 20 µl from each well of the second plate were transferred to micro reaction vessels and the peptide solutions were diluted with the calculated amounts of 20% ethanol (v/v) in water in order to obtain 50 µM stock solutions of peptide/FAM conjugates.

Ramaker K., Henkel M., Krause T., Röckendorf N, & Frey A. (2018). Cell penetrating peptides: a comparative transport analysis for 474 sequence motifs. Drug Delivery, 25(1), 928-937.

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