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3 protocols using quantum dots qd655

1

Polymeric Nanoparticle Synthesis and Characterization

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Poly (methyl methacrylate-co-methacrylic acid) (PMMA-MA, 1.3% methacrylic acid, Mn ∼15000, Mw ∼34000), acetonitrile (anhydrous, 99.8%), rhodamine B octadecyl ester perchlorate (>98.0%), lithium tetrakis(pentafluorophenyl)borate ethyl etherate were purchased from Sigma-Aldrich. DiD oil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate) (Cy5) and quantum dots QD655 were purchased from Life-Technologies. Sodium phosphate monobasic (>99.0%, Sigma-Aldrich) and sodium phosphate dibasic dihydrate (>99.0%, Sigma-Aldrich) were used to prepare 20 mM phosphate buffers at pH 5.8-9.0. Milli-Q water (Millipore) was used in all experiments.
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2

Polymeric Nanoparticle Synthesis and Characterization

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Poly (methyl methacrylate-co-methacrylic acid) (PMMA-MA, 1.3% methacrylic acid, Mn ∼15000, Mw ∼34000), acetonitrile (anhydrous, 99.8%), rhodamine B octadecyl ester perchlorate (>98.0%), lithium tetrakis(pentafluorophenyl)borate ethyl etherate were purchased from Sigma-Aldrich. DiD oil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate) (Cy5) and quantum dots QD655 were purchased from Life-Technologies. Sodium phosphate monobasic (>99.0%, Sigma-Aldrich) and sodium phosphate dibasic dihydrate (>99.0%, Sigma-Aldrich) were used to prepare 20 mM phosphate buffers at pH 5.8-9.0. Milli-Q water (Millipore) was used in all experiments.
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3

Multimodal Optical Characterization

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Rod mirrors were from Edmund optics. Emission filters, a single mode and multi-mode optical fibres (25, 50 μm fibre core), fibre-coupled warm white light and narrow-band (365, 470 nm) LEDs were from Thorlabs. 632 nm laser was from Melles Griot (model # 05-LHP-991). Green fluorescent polystyrene beads and Quantum dots QD655 were from Life Technologies. 100 nm gold nanospheres were from Nanopartz. Smaller gold nanoparticles were from BBI International. Polystyrene nanospheres were from Polysciences.
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