Prior to analysis by flow cytometry, the cells were labeled with the following antibodies or dyes: FITC anti-human CD45 Antibody (1:100, Biolegend, 304006), Brilliant Violet 605 anti-human CD14 (1:100, Biolegend, 301834), Brilliant Violet 421 anti-human ITGAM (1:100, Biolegend, 301324), PE anti-human CD68 Antibody (1:100, Biolegend, 333807), DRAQ5 (1:100, Biolegend, 424101), BUV395 Mouse Anti-Human ITGAX (CD11b) (1:100, BD Biosciences, 563787). Cells were measured using a FACSAria flow cytometer (BD Biosciences) and data were analyzed using FlowJo (Tree Star).
Pe anti human cd68 antibody
Manufactured by BioLegend
The PE anti-human CD68 Antibody is a laboratory reagent used for the detection and analysis of CD68, a glycoprotein expressed on the surface of macrophages and monocytes. This antibody is conjugated with the fluorescent dye Phycoerythrin (PE), allowing for the identification and quantification of CD68-positive cells through flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Fitc anti human cd45 antibody, by BioLegend (1 mentions)
Draq5, by BioLegend (1 mentions)
Facsaria, by BD (1 mentions)
Fc receptor blocking solution, by BioLegend (1 mentions)
Fitc conjugated secondary antibody, by Abcam (1 mentions)
Anti mertk antibody, by Cell Signaling Technology (1 mentions)
Ix83 microscope, by Olympus (1 mentions)
2 protocols using pe anti human cd68 antibody
1
Multiparameter Flow Cytometry Analysis
2
Immunofluorescence Analysis of MERTK in IPF Macrophages
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Lung sections of IPF patients and healthy controls (HCs) were permeabilized with 0.25% Triton X-100 in PBS. After blocking with 10% goat serum and Fc receptor blocking solution (1:25 dilution, BioLegend), lung sections were incubated with anti-Mertk antibody (1:50 dilution, Cell Signaling Technology) overnight at 4 °C, followed by staining with FITC-conjugated secondary antibody (1:500 dilution, Abcam) for 1 h at room temperature. Lung macrophages were labelled with PE anti-human CD68 antibody (1:25 dilution, BioLegend) for 30 min at 4 °C. DAPI was used as a nuclear counterstain. Images of Mertk in CD68+ cells were acquired with an Olympus IX83 microscope. The fluorescence intensity of MERTK in CD68+ cells was analysed by ImageJ software (NIH, Bethesda, MA, USA).
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