Brain imaging data were obtained using a Discovery MR750w 3.0T scanner (General Electric Healthcare, Milwaukee, WI, USA). Structural T1 images were acquired using a sagittal three-dimensional fast spoiled gradient recalled echo acquisition protocol with the following parameters: Image matrix = 256 mm × 230 mm; voxel size = 1 mm3; repetition time (TR) = 8.5 ms for 11 patients and 11 controls, and 8.6 ms for 4 patients and 4 controls; echo time (TE) = 3.2 ms; field of view (FOV) = 256 mm. The number of sagittal slices spanning the whole brain differed slightly between subjects: 162, 166, 170, 174, 178, or 182 slices were acquired depending on the brain size. DTI were acquired using a single shot pulsed gradient dual echo acquisition protocol with the following parameters: axial acquisition = 128 mm × 128 mm; voxel size = 0.9 × 0.9 × 4 mm3; TR = 8000 ms. TE differed slightly between subjects, ranging from 76.1 to 85.0 ms. The FOV was 240 mm, and the flip angle was 90°. The number of axial slices of the whole brain differed slightly between subjects: 34, 35, 36, or 37 slices were acquired, depending on the brain size. All DTI images had 15 distributed orientations at a b-value of 1000 s/mm2, with one b = 0 image.
Discovery mr750w 3.0t scanner
Manufactured by GE Healthcare
Sourced in United States
The Discovery MR750w 3.0T scanner is a magnetic resonance imaging (MRI) system manufactured by GE Healthcare. It operates at a magnetic field strength of 3.0 Tesla, enabling high-quality imaging capabilities. The core function of the system is to acquire and process MRI data for diagnostic and clinical applications.
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10 protocols using discovery mr750w 3.0t scanner
1
Multimodal Brain Imaging Protocol
2
Functional MRI Acquisition and Setup
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Imaging data were collected using a Discovery MR750w 3.0T scanner (GE Medical Systems, Milwaukee, WI, USA) at the UZ Brussel hospital (Belgium). A T2*-weighted spin echo planar imaging sequence was used: repetition time (TR) = 3000 ms, echo time (TE) = 70 ms, flip angle = 90°, matrix size = 128 × 128 × 27 and voxel size = 1.88 × 1.88 × 5 mm3. Two initial dummy scans were acquired and then discarded for the analysis. Twenty-seven slices were acquired in an ascending and interleaved order, covering the whole brain. Anatomical images were obtained using a T1-weigthed sagittal three-dimensional TFE (turbo field echo) sequence: TR = 8.644 ms, TE = 3.244 ms, inversion time = 450 ms, flip angle = 12°, field of view = 240 × 240 mm2, matrix size = 256 × 256 × 128 and voxel size = 0.94 × 0.94 × 1.2 mm3. Stimuli were displayed on an MR-compatible backward projection screen visible to the participant through a mirror in the MR head coil. Stimuli were presented using MATLAB (The MathWorks). Motor responses were recorded using a standard MRI-compatible response device (fORP, Current Designs, USA).
3
Neuroimaging Protocol for Resting-State fMRI Analysis
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Imaging data were acquired on a Discovery MR 750w 3.0-T scanner (GE Healthcare, Milwaukee, WI) at the Tokyo Metropolitan Institute of Gerontology. High-resolution anatomical data were collected using an SPGR sequence (repetition time = 7.648 ms, echo time = 3.092 ms, flip angle = 11°, matrix size = 196 × 256 × 256, voxel size = 1.2 mm × 1.0547 mm × 1.0547 mm). Whole-brain resting-state fMRI data were collected using an echo planar imaging (EPI) sequence (repetition time = 2500 ms, echo time = 30 ms, flip angle = 73°, slice thickness = 4 mm, matrix size = 64 × 64 × 41, FOV = 192 mm × 192 mm). The participants were instructed to rest quietly with their eyes open and to avoid specific thoughts during the resting-state fMRI sessions. Subsequently, the procedure was manually reviewed to verify that all participants followed the instructions correctly.
4
Spinal MRI Lesion Measurement
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Brain and whole spine MRIs (GE SIGNA™ HDxt 1.5 T scanner or GE Discovery™ MR750w 3.0 T scanner) were conducted in all patients. Those patients with suspected demyelinating brain lesions were excluded from this study. MRI lesion length was independently measured as vertebral body segments of the longest hyperintense lesion on sagittal T2-weighted images by two authors (S.Y.K. and S.-Y.S.). Any discrepancies were resolved through discussion to reach a consensus.
5
MRI Protocols for Neoadjuvant Chemoradiotherapy
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Patients received baseline MR scan in 2 weeks before nCRT. Fat-suppressed T2WI were accessed from two institutions. At institution 1, all MR examinations were performed using a GE Discovery MR750w 3.0T scanner or a GE Discovery MR750 3.0T scanner. At institution 2, MR examinations were performed using four scanners (SIEMENS Skyra 3.0T scanner, SIEMENS Avanto 1.5T scanner, UIH uMR780 3.0T scanner, and UIH uMR588 1.5T scanner). Details of all scanner protocols are provided in eTable 1 .
6
Multimodal MRI Acquisition Protocol
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All participants were scanned on a General Electric Discovery MR750w 3.0 T scanner (General Electric, Waukesha, WI, USA) with a 24-channel head coil. MRI examinations were performed within 2 days after the completion of the cognitive tests. The MR imaging protocol included routine T2-weighted and fluid-attenuated inversion recovery (FLAIR) images, three-dimensional (3D) high-resolution T1-weighted structure images, and resting-state functional MRI (rs-fMRI) images. Routine T2-weighted and FLAIR images were used to exclude subjects with organic brain diseases. 3D high-resolution T1-weighted structure images were acquired using the brain volume (BRAVO) sequence (TR = 8.5 ms; TE = 3.2 ms; TI = 450 ms; flip angle = 12°; 188 slices; slice thickness = 1 mm; no gap; FOV = 256 × 256 mm2; matrix = 256 × 256). The rs-fMRI scans were performed with an echo planar imaging sequence (TR = 2000 ms; TE = 30 ms; flip angle = 90°; slice thickness = 3 mm; slice gap = 1 mm; FOV = 220× 220 mm2; matrix = 64 × 64). A custom-built head coil cushion and earplugs were used to minimize head motion and dampen scanner noise. During the scans, subjects were instructed to keep their eyes closed, relax, and move as little as possible while not falling asleep.
7
3T Brain Imaging Protocol
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Brain imaging data was collected using a Discovery MR750w 3.0T scanner (GE Medical Systems, Milwaukee, Wisconsin, USA) at UZ Brussel. Anatomical images were obtained using a T1-weigthed sagittal 3D TFE (turbo field echo) sequence: repetition time = 8.644 ms, echo time = 3.244 ms, inversion time = 450 ms, flip angle = 12°, field of view = 240x240 mm2, matrix size = 256 x 256 x 128 and voxel size = 0.94x0.94x1.2 mm3.
8
Structural Brain Imaging with 3T MRI
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Brain imaging data were obtained using a Discovery MR750w 3.0 T scanner (General Electric Healthcare, Milwaukee, WI) equipped with a 32-channel head coil. Structural T1 images were acquired using a sagittal three-dimensional fast spoiled gradient-recalled-echo acquisition protocol with the following parameters: image matrix, 256 × 230 mm; voxel size, 1 mm3; field of view, 256 mm; flip angle, 12 degrees; repetition time, 8.5 msecs for 18 patients and 16 controls, 8.6 msecs for nine patients and six controls, 8.4 msecs for one patient and two controls, and 8.7 msecs for one control; and echo time, 3.2 msecs for all patients and controls. Two controls had the same parameters with repetition time of 8.5 and 8.6 msecs and image matrix of 256 × 256 mm. The numbers of sagittal slices spanning the whole brain differed slightly among the subjects, depending on brain size (from 146 to 182 slices).
9
Multimodal MRI Neuroimaging Protocol
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All participants were scanned on a General Electric Discovery MR750w 3.0T scanner (General Electric, Waukesha, WI, USA) with a 24-channel head coil. MRI examinations were performed within 2 days after the completion of the cognitive tests. The MR imaging protocol included routine T2-weighted and uidattenuated inversion recovery (FLAIR) images, three-dimensional (3D) high-resolution T1-weighted structure images, and resting-state functional MRI (rs-fMRI) images. Routine T2-weighted and FLAIR images were used to exclude subjects with organic brain diseases. 3D high-resolution T1-weighted structure images were acquired using the brain volume (BRAVO) sequence (TR = 8.5 ms; TE = 3.2 ms; TI = 450 ms; ip angle = 12°; 188 slices; slice thickness = 1 mm; no gap; FOV = 256 × 256 mm 2 ; matrix = 256 × 256). The rs-fMRI scans were performed with an echo planar imaging sequence (TR = 2000 ms; TE = 30 ms; ip angle = 90°; slice thickness = 3 mm; slice gap = 1 mm; FOV = 220× 220 mm 2 ; matrix = 64 × 64). A custom-built head coil cushion and earplugs were used to minimize head motion and dampen scanner noise. During the scans, subjects were instructed to keep their eyes closed, relax, and move as little as possible while not falling asleep.
10
Healthy Volunteer Brain Imaging Study
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Subjects were recruited using the healthy volunteer database of 3H Clinical Trial (Tokyo, Japan). Cohort A included 3 healthy male subjects (43.3 ± 10.7 years) and cohort B included 5 healthy male subjects (39.2 ± 13.2 years). For anatomical coregistration, MRI was performed in cohort B using a Discovery MR750w 3.0 T scanner (GE Healthcare, Milwaukee, WI). A 3-dimensional (3D) fast spoiled gradient-echo (repetition time, 7.6 ms; echo time, 3.1 ms; inversion time, 400 ms; matrix, 256 × 256 × 196 voxels) T1-weighted whole-brain image was acquired for each subject.
The main exclusion criteria in cohorts A and B were abnormal physical or neurological examination or paraclinical investigations (biochemistry, haematology and cardiovascular systems using standard laboratory tests and electrocardiograms), history of significant medical illness including major internal pathology or neurological and neuropsychiatric disorders, history of severe drug hypersensitivity, 1 3 smokers, significant abnormalities on anatomical MRI and the use of prescribed pharmaceuticals, general pharmaceuticals, quasi-drugs and health food products that induce, inhibit, or act as a substrate for P-gp. Subjects were asked to not drink alcohol on the day prior to the scan or on the scan day itself.
The main exclusion criteria in cohorts A and B were abnormal physical or neurological examination or paraclinical investigations (biochemistry, haematology and cardiovascular systems using standard laboratory tests and electrocardiograms), history of significant medical illness including major internal pathology or neurological and neuropsychiatric disorders, history of severe drug hypersensitivity, 1 3 smokers, significant abnormalities on anatomical MRI and the use of prescribed pharmaceuticals, general pharmaceuticals, quasi-drugs and health food products that induce, inhibit, or act as a substrate for P-gp. Subjects were asked to not drink alcohol on the day prior to the scan or on the scan day itself.
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