Fungi were mainly cultivated in basic medium containing the following (per L): 0.5 g KH
2PO
4, 0.5 g MgSO
4·7H
2O, 0.0915 g FeSO
4·7H
2O, 0.8 g (NH
4)
2SO
4, 1.0 g corn steep liquor (CSL, Sigma-Aldrich, St. Louis, MO, USA) and 100 g glucose. Glucose was mainly used as a carbon source. All the reagents used, except CSL, were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). The initial pH of the medium was adjusted to 2–4 using HCl aq. before sterilization. The medium was autoclaved at 121 °C for 15 min. The carbon sources used in this study were hexoses (glucose, galactose, and fructose), pentoses (xylose and arabinose), and disaccharides (sucrose and lactose) at an initial concentration of 100 g/L. The nitrogen sources of the natural extracts were
casamino acid,
casein (Sigma-Aldrich),
malt extract (Difco Laboratories), meat extract (Kyokuto Pharmaceutical Industrial Co. Ltd., Tokyo, Japan), and Hipolypepton (Nihon Pharmaceutical Co. Ltd., Tokyo, Japan). The salts used as nitrogen source were KNO
3, NaNO
3, NH
4NO
3, (NH
4)
2SO
4, and
urea (FUJIFILM Wako Pure Chemical Co.), which were added to the basic medium at a concentration of 5 g/L. The mineral components used were CaCl
2, CoCl
2, CuSO
4, FeCl
2, FeCl
3, FeSO
4, Fe
2(SO
4)
3, MnSO
4, NiCl
2, ZnCl
2, and ZnSO
4, which were added to the basic medium at a concentration of 5 mg/L.
Moriwaki-Takano M., Asada C, & Nakamura Y. (2021). Production of spiculisporic acid by Talaromyces trachyspermus in fed-batch bioreactor culture. Bioresources and Bioprocessing, 8(1), 59.