Cd271 pe

Manufactured by BD

Sourced in United States

CD271-PE is a laboratory reagent that detects the CD271 (p75 NGFR) cell surface marker using flow cytometry. It is a fluorescently labeled antibody that binds to the CD271 antigen expressed on certain cell types.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using cd271 pe

Isolation and Characterization of Human Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HuMSCs were isolated and cultured according to methods previously described^{5 (link), 36 (link)}. HuMSCs were cultured in α-MEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (FBS, Life Technologies, USA) and 1% penicillin and streptomycin (100×, Life Technologies) at 37 °C, 5% CO₂ and 100% H₂O.
To observe morphology, adherent cells were stained with Wright’s stain and imaged with a JEOL-1200EX transmission electron microscope, and images were recorded with a MORADA-G2 camera.
To detect typical surface markers of huMSCs, FACS was performed using the following phycoerythrin (PE)-conjugated, fluorescein isothiocyanate (FITC)-conjugated, percp-Cy5.5-conjugated or allophycocyanin (APC)-conjugated mouse antihuman monoclonal antibodies: PE-CD29, PE-CD31, PE-CD34, FITC-CD44, PE-CD45, PE-CD73, PerCp-Cy5.5-CD90, PE-CD105, PE-CD271, APC-CD133, PE-HLA-DR and PE-HLA-ABC (BD Biosciences, Franklin Lakes, NJ, USA). HuMSCs were collected in 100 µl of PBS. Every tube contained 1 × 10⁵ cells, and antibodies were added. After incubation at room temperature for 20 min, the cells were examined with a Guava easyCyte HT flow cytometer (Millipore, Billerica, MA, USA). The results were analysed with guavaSoft 3.1.1 software; n = 5.

Sun L., Li D., Song K., Wei J., Yao S., Li Z., Su X., Ju X., Chao L., Deng X., Kong B, & Li L. (2017). Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro. Scientific Reports, 7, 2552.

+ Open protocol

+ Expand

Immunophenotyping of DFAT Cells by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The immunophenotype of DFAT cells at passage 2 was identified using flow cytometry as previously described (23) . The cells grown to 60% confluence were suspended at a density of 5 × 10 5 cells per tube and labeled using antibodies for the following antigens: CD13 (PE-CD13, aminopeptidase N), CD44 (FITC-CD44, hyaluronate recptor, and phagocytic glycoprotein-1), CD45 (FITC-CD45, leucocyte common antigen), CD73 (PE-CD73, NT5E), CD90 (APC-CD90, Thy-1), CD105 (APC-CD105, endoglin), CD271 (PE-CD271, NGFR), and STRO-1 (FITC-STRO1) (all from BD Biosciences, San Jose, CA, USA). Also, control cells were incubated with isotype-matched mouse anti-human IgGs and used as negative controls. Dead cells were identified by staining with 1 μg/mL propidium iodide (Sigma-Aldrich). Flow cytometry data were analyzed using FlowJo software (Tree Star Inc., Ashland, OR, USA).

Tsurumachi N., Akita D., Kano K., Matsumoto T., Toriumi T., Kazama T., Oki Y., Saito-Tamura Y., Tonogi M., Shimizu N, & Honda M. (2018). Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad. Journal of oral science, 60(1).

+ Open protocol

+ Expand

Lentiviral Transduction of Leukemia Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

GIPZ HIF-1α or control shRNA plasmids were from Open Biosystems. Lentiviral vectors were obtained by HEK-293T transfection as previously described [55 (link)]. MOLM-13, THP-1 and Kasumi-1 cells were transduced by spinoculation, selected with puromycin (4 μg/mL, 7 μg/mL and 1 μg/mL respectively) and sorted for GFP expression 2 weeks after transduction (MoFlo XDP, Beckman Coulter), leading to a bulk of cells with different integrations and GFP levels.
For in vivo experiments, shCTRL and shHIF-1α MOLM-13 cells were further transduced with a bidirectional lentiviral vector co-expressing luciferase and truncated nerve growth factor receptor (ΔNGFR) (kindly provided by G. Escobar, B. Gentner and L. Naldini, unpublished data) and sorted for ΔNGFR (CD271, PE, BD Pharmingen) expression 2 weeks after transduction.
For in vivo experiments with LNA-ON (EZN-3088 and EZN-2968), MOLM-13 cells transduced with the bidirectional lentiviral vector co-expressing luciferase and ΔNGFR and sorted for ΔNGFR expression 2 weeks after transduction, were electroporated with EZN-3088 and EZN-2968 and injected in mice after 24 hours.

Migliavacca J., Percio S., Valsecchi R., Ferrero E., Spinelli A., Ponzoni M., Tresoldi C., Pattini L., Bernardi R, & Coltella N. (2016). Hypoxia inducible factor-1α regulates a pro-invasive phenotype in acute monocytic leukemia. Oncotarget, 7(33), 53540-53557.

+ Open protocol

+ Expand

Comprehensive T cell phenotyping by flow cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

T cell basal and post-activation phenotypes were characterized by flow cytometry staining with FITC, PE, PerCP, PeCy7, APC, APC-H7, Pacific Blue, BV510 conjugated antibodies, and analyzed with a FACS Canto II flow cytometer (BD Biosciences). CAR T cells and mouse samples were stained with one or more of the following conjugated monoclonal antibodies: anti-human CD3 PB (Biolegend, clone HIT3a), CD45 BV510 (Biolegend, clone HI30), CD271 PE-Cy7 (Biolegend, clone CD40-1457), CD271 PE (BD, clone C40-1457), CD4 FITC (Biolegend, clone SK3), CD14 APC (Biolegend, clone M5E2), CD19 APC/Cy7 (Biolegend, clone HIB19), HLA-DR APC/Cy7 (Biolegend, clone L243), CD45RA FITC (Biolegend, clone HI100), CD62L APC (Biolegend, clone DREG-56), CD8 PerCP (BD, clone SK1), CD57 APC/Cy7 (Milteny, clone TB03), CD127 PE (Biolegend, clone A019D5), EGFR PE (Biolegend, clone AY13), CD95 (Fas/APO-1) PE-Cy7 (Biolegend, clone DX2), CD279 (PD-1) PE-Cy7 (Biolegend, clone EH12.1), TIM-3 Alexa Fluor 488 (Biolegend, clone F38-2E2), and anti-mouse CD45 PerCP (Biolegend, clone 30-F11). 7-Aminoactinomicin D (7-AAD, Biolegend) and DAPI were used to discriminate viable and non-viable cells.
All data were analyzed with the Flow Jo_V10 software (Tree Star Inc.).

Arcangeli S., Falcone L., Camisa B., De Girardi F., Biondi M., Giglio F., Ciceri F., Bonini C., Bondanza A, & Casucci M. (2020). Next-Generation Manufacturing Protocols Enriching TSCM CAR T Cells Can Overcome Disease-Specific T Cell Defects in Cancer Patients. Frontiers in Immunology, 11, 1217.

+ Open protocol

+ Expand

Immunoregulatory Molecule Expression in ASCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The surface expression of immunoregulatory-related molecules was evaluated for ASCs, at the second or third passage, by flow cytometric analysis using the following fluorochrome-labeled monoclonal antibodies: CD200-PE, CD271-PE, CD274-FITC, and CD276-FITC (BD, Biosciences, USA). Flow cytometry was performed using a Beckman Coulter EPICS XL device (Beckman Coulter, USA) and data analysis was carried out using System II software.

Abu-Shahba N., Mahmoud M., Abdel-Rasheed M., Darwish Y., AbdelKhaliq A., Mohammed E., ElHefnawi M, & Azmy O. (2020). Immunomodulatory and Antioxidative potentials of adipose-derived Mesenchymal stem cells isolated from breast versus abdominal tissue: a comparative study. Cell Regeneration, 9, 18.

+ Open protocol

+ Expand

Canine Mesenchymal Stem Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal care and ABMC studies were carried out according to guidelines of the animal care committee of both Rutgers University and the Faculty of Medicine at Cairo University. Twenty-two adult male mixed-breed dogs (3 to 4 years old) were obtained from local vendors and housed at the vivarium of the Faculty of Veterinary Medicine at Cairo University. Canine ABMCs were isolated from the femurs of six adult dogs for in vitro studies, as we recently described for human ABMCs. Canine ABMCs were subjected to flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA) after staining with 100 μl of labeled antibodies (appropriately diluted to previously determined titration points) against the following cell surface markers: cluster of differentiation 13-phyco-erythrin (PE) cyanine 7 (cy7) (CD13-PC7), CD29-PC7, CD34-PE, CD44-fluorescein isothiocyanate (FITC), CD45-PC7, CD73-PE, CD90-PE, CD105-PE, CD166-PE, CD271-PE, and c-kit-PE (all from BD Biosciences). Dead cells were excluded by labeling with 1 μg/ml of 7-aminoac-tinomycin D (7-AAD; Invitrogen, Carlsbad, CA, USA). Mesenchymal induction into osteogenic, adipogenic, and chondrogenic lineages was performed as previously described (18 (link),34 (link)). Green fluorescent protein (GFP) labeling and neural induction were performed as previously described (2 ,9 (link)) with modifications described below.

Gabr H., El-kheir W.A., Farghali H.A., Ismail Z.M., Zickri M.B., El Maadawi Z.M., Kishk N.A, & Sabaawy H.E. (2014). Intrathecal Transplantation of Autologous Adherent Bone Marrow Cells Induces Functional Neurological Recovery in a Canine Model of Spinal Cord Injury. Cell transplantation, 24(9), 1813-1827.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!