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Cddo me

Manufactured by Merck Group
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Sourced in Germany, United States

CDDO-Me is a synthetic triterpenoid compound developed by Merck Group. It functions as a potent activator of the Nrf2 transcription factor, which plays a crucial role in the regulation of antioxidant and cytoprotective genes.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Acetonitrile, by Merck Group (2 mentions) Methanol, by Merck Group (2 mentions) Menadione, by Merck Group (1 mentions) Ucf 101, by Merck Group (1 mentions) Bapta, by Abcam (1 mentions) Mitoblock 6, by Focus Biomolecules (1 mentions) 2 deoxyglucose, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Abcam (1 mentions) Bapta am, by Abcam (1 mentions) Ionomycin, by Abcam (1 mentions) Ac devd cho, by Selleck Chemicals (1 mentions) Cddo me, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Biosera (1 mentions) Dmem f12, by Fujifilm (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Human serum, by Merck Group (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)

12 protocols using cddo me

1

Cell Treatment Chemicals Preparation

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Chemicals used for the treatment of cells were prepared as followed, with final dilutions made with the respective cell culture medium. FCCP (Abcam, Cambridge, UK), 1 mm in DMSO, MPP+(Sigma-Aldrich), 10 mm in water, ionomycin (ab120370, Abcam) 10 mm, and 1 mm in DMSO, 2-deoxyglucose (Sigma-Aldrich) 0.5 m in water, menadione (Sigma-Aldrich), 1.5 mm in DMSO, BAPTA-AM (ab120503, Abcam), 2.5 mm in DMSO, BAPTA (ab144924, Abcam), 1 mm in water, CDDO-Me (Sigma-Aldrich), 1 mm in DMSO, UCF-101 (Sigma-Aldrich), 10 mm in DMSO, MitobloCK-6 (Focus Biomolecules), 5 mm in DMSO, bafilomycin A1 (Calbiochem, San Diego, CA, USA), 100 and 10 μm in DMSO, 17-AAG (ab141433, Abcam), 5 mm and 50 μm in DMSO and MG132 (Sigma-Aldrich), and 10 and 1 mm in DMSO.
Lautenschläger J., Wagner-Valladolid S., Stephens A.D., Fernández-Villegas A., Hockings C., Mishra A., Manton J.D., Fantham M.J., Lu M., Rees E.J., Kaminski C.F, & Kaminski Schierle G.S. (2020). Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β1-42 pathologies. The Journal of Biological Chemistry, 295(30), 10138-10152.
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2

Cisplatin-Induced Apoptosis in Renal Cells

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The human renal proximal tubular epithelial cell line HK-2 was cultured in Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F-12 (DMEM/F12, Fujifilm Wako Chemical Co., Osaka, Japan) supplemented with 10% fetal bovine serum (FBS, Biosera, Inc., Nuaille, France) and 1% penicillin–streptomycin, as previously described [34 ]. Cells were treated with 0–50 µmol/L cisplatin in low-glucose DMEM containing 0.1 mg/mL human serum albumin for 6 h. The medium was then replaced with fresh medium with or without 0.1–0.2 μmol/L CDDO-Me (Sigma-Aldrich Co., St. Louis, MO, USA). The cells were treated with 5 or 50 µmol/L of the caspase inhibitor, Ac-DEVD-CHO (Selleckchem, Houston, TX, USA), for 60 min before adding CDDO-Me to the medium. Cells were cultured for 24–72 h, and proteins or mRNA were extracted at the indicated points.
Kurosaki Y., Imoto A., Kawakami F., Ouchi M., Morita A., Yokoba M., Takenaka T., Ichikawa T., Katagiri M., Nielsen R, & Ishii N. (2022). In vitro study on effect of bardoxolone methyl on cisplatin-induced cellular senescence in human proximal tubular cells. Molecular and Cellular Biochemistry, 477(3), 689-699.
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3

Monocyte Isolation and Cytokine Stimulation

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5 x 105 PBMCs were plated in 200 μL of RPMI 1640 (ThermoFisher, 1875093) with 2% Human Serum (Sigma, H3667), Beta-mercaptoethanol(Gibco, 21985–027), Sodium Pyruvate(Gibco, 11360), Non-essential Amino Acids (Gibco, 11140–076) and antibiotics (Gibco, 152140–002) in 96-well plates (Fisher, 351177). Monocyte purification was performed with CD14 microbeads(Miltenyi, 130-050-201) according to the manufacturer’s directions. Purified monocytes were re-suspended in RPMI media(See above) and plated the same as PBMCs. PBMCs and monocytes were treated with CDDO-Me(bardoxolone methyl)(Sigma-Aldrich, SMB00376), Vehicle (0.00005% DMSO), Compound 7 [21 (link)], or Vehicle (0.001% DMSO) for 1 hour before stimulation with 5 ng/mL of Lipopolysaccheride (LPS) (Sigma-Aldrich, L2630) or IL-6 (50 ng/mL, Peprotech, 200–06). At indicated times post-stimulation, plates were centrifuged at 500 x g for 5 minutes and supernatants were collected. Cell pellets were subjected to protein or RNA analysis (See below).
Eitas T.K., Stepp W., Sjeklocha L., Long C., Riley C., Callahan J., Sanchez Y., Gough P., Knowlin L., van Duin D., Ortiz-Pujols S., Jones S., Maile R., Hong Z., Berger S, & Cairns B. (2017). Differential regulation of innate immune cytokine production through pharmacological activation of Nuclear Factor-Erythroid-2-Related Factor 2 (NRF2) in burn patient immune cells and monocytes. PLoS ONE, 12(9), e0184164.
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4

Preparation of Bardoxolone Methyl Solution

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Analytical grade 2-Cyano-3,12-dioxo-oleana-1,9(11)-dien-28-oic acid methyl ester (CDDO-Me; Bardoxolone methyl) was purchased from Sigma-Aldrich (#SMB00376; Sigma-Aldrich, Munich, Germany). The compound has a molecular weight of 505.7 g/moL and chemical structure of the Bardoxolone methyl ester, as shown in the PubChem Database (Pub Med CID: 400769; see Supplementary Figure S1). CDDO-Me was dissolved in dimethyl sulfoxide (DMSO; #D8418; Sigma-Aldrich, Munich, Germany) to a final concentration of 1 mg/mL. The stock solution was aliquoted in a volume of 100 µL/tube and stored at −20 °C.
Amirova K.M., Dimitrova P.A., Leseva M.N., Koycheva I.K., Dinkova-Kostova A.T, & Georgiev M.I. (2023). The Triterpenoid Nrf2 Activator, CDDO-Me, Decreases Neutrophil Senescence in a Murine Model of Joint Damage. International Journal of Molecular Sciences, 24(10), 8775.
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5

Antiviral Compound Screening for DENV and ZIKV

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All viruses were obtained from Emerging Infections Research Resources Repository (BEI Resources, Manassas, VA, USA). Monkey plasmas neutralizing DENV or ZIKV were provided by Dr. Gregory Gromowski, Viral Diseases Branch, Walter Reed Army Institute of Research. Aedes albopictus mosquito C6/36 cells (ATCC CRL-1660), African green monkey kidney epithelial Vero cells (CCL-81), human embryonic kidney HEK-293 cells (CRL-1573), SH-SY5Y cells (CRL-2266), U-251 MG cells (HBT-17), and HeLa cells (CCL-2) were obtained from ATCC (Manassas, VA, USA). Huh-7 cells were provided by Dr. Hongbing Wang, University of Maryland School of Pharmacy. All cells were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) as growth medium or 2% FBS as maintenance medium. Bortezomib (BTZ), CDDO-me, and PU-WS13 were purchased from Sigma-Aldrich (St. Louis, MO, USA), Adooq Biosciences (Irvin, CA, USA), EMD Chemicals (San Diego, CA, USA), respectively. The sources for antibodies are: Anti-grp94 antibody (Affinity Bioreagents, Golden, CO, USA), anti-OS9 antibody (Thermo Fisher Scientific, Waltham, MA, USA), Anti-Hsp70 and anti-Tubulin antibodies (Enzo Life Sciences, Farmingdale, NY, USA), anti-Hsp90 and anti-BiP antibodies ( BD Biosciences, San Jose, CA, USA), Anti-DENV2 E protein and anti-DENV2 NS3 protein antibodies (GeneTex, San Antonio, TX, USA).
Rothan H.A., Zhong Y., Sanborn M.A., Teoh T.C., Ruan J., Yusof R., Hang J., Henderson M.J, & Fang S. (2019). Small molecule grp94 inhibitors with antiviral activity against Dengue and Zika virus. Antiviral research, 171, 104590.
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6

CDDO-Me Treatment Regimen for LPS-Induced Inflammation

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2-Cyano-3,12-dioxo-oleana-1,9(11)-dien-28-oic acid methyl ester (CDDO-Methyl ester or CDDO-Me, Sigma-Aldrich) was dissolved in DMSO to produce a 10 mM stock solution. The stock solution was further diluted with 7.5% PBST (1.5 ml Tween-20 dissolved in 20 ml PBS) to obtain a 200 nM CDDO-Me solution. Mice received 100 µL of CDDO-Me solution via intraperitoneal injection administered once daily for 3 days beginning 1 day after intracranial administration of LPS.
Kim K., Kim H., Bae S.H., Lee S.Y., Kim Y.H., Na J., Lee C.H., Lee M.S., Ko G.B., Kim K.Y., Lee S.H., Song I.H., Cheon G.J., Kang K.W., Kim S.E., Chung J.K., Kim E.E., Paek S.H., Lee J.S., Lee B.C, & Youn H. (2020). [18F]CB251 PET/MR imaging probe targeting translocator protein (TSPO) independent of its Polymorphism in a Neuroinflammation Model. Theranostics, 10(20), 9315-9331.
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7

CDDO-Me Compound Protocol

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CDDO-Me (2-Cyano-3,12-dioxo-oleana-1,9(11)-dien-28-oic acid methyl, or bardoxolone methyl) was purchased from Sigma Aldrich. Stock solutions were resuspended in dimethyl sulfoxide (DMSO).
Fahrmann J.F., Tanaka I., Irajizad E., Mao X., Dennison J.B., Murage E., Casabar J., Mayo J., Peng Q., Celiktas M., Vykoukal J.V., Park S., Taguchi A., Delgado O., Tripathi S.C., Katayama H., Soto L.M., Rodriguez-Canales J., Behrens C., Wistuba I., Hanash S, & Ostrin E.J. (2022). Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma. Cancers, 14(10), 2543.
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8

Modulating NRF2 Expression Levels

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To downregulate NRF2 expression, ML385 (Sigma–Aldrich; Merck KGaA, Darmstadt, Germany), a specific NRF2 inhibitor, was added into the culture medium at a concentration of 5 μM, followed by incubation for 48 h. Meanwhile, CDDO-Me (Sigma–Aldrich; Merck KGaA, Darmstadt, Germany), a specific NRF2 activator, was used to upregulate NRF2 expression by incubating for 48 h at a concentration of 0.5 μM.
Zhang J., Zhang J., Ni H., Wang Y., Katwal G., Zhao Y., Sun K., Wang M., Li Q., Chen G., Miao Y, & Gong N. (2021). Downregulation of XBP1 protects kidney against ischemia-reperfusion injury via suppressing HRD1-mediated NRF2 ubiquitylation. Cell Death Discovery, 7, 44.
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9

Bardoxolone Methyl Preparation Protocol

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CDDO-Me (2-Cyano-3,12-dioxo-oleana-1,9(11)-dien-28-oic acid methyl, or bardoxolone methyl) was purchased from Sigma Aldrich (Cat# SMB00376). Stock solutions were resuspended in dimethyl sulfoxide (DMSO).
León-Letelier R.A., Abdel Sater A.H., Chen Y., Park S., Wu R., Irajizad E., Dennison J.B., Katayama H., Vykoukal J.V., Hanash S., Ostrin E.J, & Fahrmann J.F. (2023). Kynureninase Upregulation Is a Prominent Feature of NFR2-Activated Cancers and Is Associated with Tumor Immunosuppression and Poor Prognosis. Cancers, 15(3), 834.
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10

Ex vivo Expansion of Endothelial Progenitor Cells

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Ex vivo expansion of early endothelial progenitor cells (eEPCs) was performed exactly as described in our previous work. Briefly, mononuclear cell fraction of the peripheral blood (PBMC) from 4-week-old healthy broiler chickens was cultured in Endothelial cell growth medium (EGM)-2 (Lonza, Walkersvil, MD, USA) containing 2% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 39 °C in 5% CO2. Non-adherent cells were removed after 48 h. On day 6 of culture, cells were passaged using 0.25% trypsin/EDTA (Invitrogen), plated on rat tail type 1 collagen-coated 6-well plates at 1  ×  107 cells/well, and treated with 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid–methyl ester (CDDO-Me) (Merck Ltd., Beijing, China) at 300 nM for 24 h with or without the addition of Brusatol (Merck Ltd., Beijing, China) at 40 nM to the culture medium 20 h after CDDO-Me exposure.
Ye L., Liu R., Li Q., Zhou C, & Tan X. (2024). Dysregulated VEGF/VEGFR-2 Signaling and Plexogenic Lesions in the Embryonic Lungs of Chickens Predisposed to Pulmonary Arterial Hypertension. International Journal of Molecular Sciences, 25(8), 4489.
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