Av400pas

¹H, ¹³C{¹H} and ³¹P{¹H} NMR spectra were recorded on Bruker AV400PAS and AV300PAS (Bruker, Karlsruhe, Germany) instruments. ¹H and ¹³C chemical shifts (δ, ppm) were measured relative to residual resonances of solvents. High-resolution mass spectra were recorded using GCT Premier (Waters, Milford, MA, USA) and UPLC Xevo G2 Q TOF (Waters, Milford, MA, USA) mass spectrometers for chemical ionization and electrospray ionization, respectively.

All manipulations were carried out using standard dry argon-high vacuum technique. Organic solvents were dried and distilled prior to use. Aldehyde-terminated phosphorus dendrimers of generations 1–3 were obtained by growing from a cyclotriphosphazene core as described in [23 (link)]. Girard T or P-modified dendrimers were synthesized according to the procedure reported for the modification of dendrimers having thiophosphate as a core [22 (link)].
¹H, ¹³C{¹H} and ³¹P{¹H} NMR spectra were recorded using AV400PAS, AV400LIQ spectrometers (Bruker, Karlsruhe, Germany). The attribution of the NMR signals of the dendrimer branches was made by analogy with Refs. [23 (link),24 (link)], the attribution of the NMR signals of the amines on the periphery was made by analogy with Ref. [22 (link)]. To assign the ¹³C NMR signals, Jmod, HMBC, HBQC NMR experiments were additionally done, if necessary. The atom numbering used for the signals attribution is given in Figure 1. Mass spectrometry was not used to prove the purity of these dendrimers because of spontaneous rearrangements in the phosphorhydrazone structure during the analysis [25 (link)], thus, the purity of the dendrimer samples was assessed only by NMR [26 (link)].