2 7 dichlorodihydrofluorescein diacetate dcfh da

Chlorin e6 (Ce6) and 10-hydroxycamptothecin (HCPT) were purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Cholesterol-b-poly(ethylene glycol) (PEG)₂₀₀₀ (Chol-PEG₂₀₀₀) was obtained from Xi’an ruixi Biological Technology Co., Ltd (Xi’an, China). Singlet oxygen sensor green reagent (SOSG) and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were supplied by Dalian Meilun Biotechnology Co., Ltd (Dalian, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma Aldrich Chemicals (Germany). RPMI-1640 was supplied by Hyclone Thermo Scientific (Waltham, MA, USA). Penicillin and streptomycin were procured from Gibco Thermo Scientific (Waltham, MA, USA). Fetal bovine serum (FBS) was obtained from Excell Bio (Shanghai, China). All the other chemical reagents were of commercially accessible analytical grade and used directly throughout the experiments without further purification.

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), triethylamine (TEA), 4-dimethylaminopyridine (DMAP), N,N-diisopropylethylamine (DIEA), N,N-dimethylacetamide (anhydrous, DMAc), dimethyl sulfoxide (anhydrous, DMSO), N,N-dimethylformamide (anhydrous, DMF), dichloromethane (anhydrous, DCM), 2,2′-azobis (2-methylpropionitrile) were purchased from J&K Scientific Ltd. (Shanghai, China). Nile Red (NiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiL) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanineiodide (DiR) were all obtained from Life Technologies (Shanghai, China). NLG919 was purchased from Selleck Chemghai, China). PPa was purchased from Dibai Chem Tech Co., Ltd. (Shanghai, China). Triphosgene, 4-cyano-4-(dodecylsulfanylthiocarbonyl) sulfanylpentanoic acid (CTA) and bis(2-hydroxyethyl) disulfide were purchased from TCI (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits were ordered from Neobioscience Technology Co., Ltd. (Shenzhen, China). Kyn, Trp and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) were ordered from Meilun Biotechnology Co., Ltd. (Dalian, China). The macromolecular chain transfer agent mPEG₁₁₃-CTA and 2-(hexamethyleneimino) ethanol methacrylate (HMA) were synthenzied by following the similar procedure reported previously^{34 ,35} (link).