The extraction of total protein from the bone tissues in the defect areas of the calvaria was performed by using the triplePrep kit (GE Healthcare Life Sciences), and its concentration was determined with a BCA assay kit. 25 μg protein per sample was separated via 10% SDS-PAGE and transferred to the PVDF membrane as described (Luo et al., 2021 (link)). After blocking in 5% skim milk at room temperature for 2 h, PVDF membranes were treated with the following primary antibodies overnight at 4°C, rabbit polyclonal anti-BMP-2 (1:1000, bs-1012R, Bioss), rabbit polyclonal anti-VEGF (1:1000, bs-1313R, Bioss), and rabbit polyclonal anti-PDGF (1:1000, bs-0196R, Bioss), followed by incubation with HRP-labeled IgG at 37°C for 2 h. The western blots were stained with an ECL reagent (Santa Cruz Biotech, CA, United States) and visualized by the ChemiDoc Touch Imaging System—Bio-Rad Laboratories (Bio-Rad Lab, Hercules, CA, United States).
Tripleprep kit
Manufactured by GE Healthcare
The TriplePrep kit is a laboratory equipment product offered by GE Healthcare. It is designed to facilitate the preparation of samples for analysis. The kit contains the necessary components to perform three distinct preparation steps, but its core function is to enable efficient sample preparation without providing interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by Santa Cruz Biotechnology (1 mentions)
Bs 0196r, by Bioss Antibodies (1 mentions)
Bs 1012r, by Bioss Antibodies (1 mentions)
Bs 1313r, by Bioss Antibodies (1 mentions)
Chemidoc touch imaging system, by Bio-Rad (1 mentions)
Mimosine, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Rhtnf α, by Thermo Fisher Scientific (1 mentions)
On target plus smartpools sirnas, by Horizon Discovery (1 mentions)
Rnaimax, by Thermo Fisher Scientific (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
U0126, by Promega (1 mentions)
2 protocols using tripleprep kit
1
Protein Extraction and Western Blot Analysis
2
Investigating Cellular Signaling Pathways
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