Plan neofluor 0.75 na ph2 dry objective

Manufactured by Zeiss

The 63× Plan Neofluor 0.75 NA Ph2 dry objective is a high-magnification optical lens designed for use in microscopy. It provides a magnification factor of 63× and a numerical aperture of 0.75, which determines the light-gathering capability of the lens. The Ph2 designation indicates that the objective is designed for phase contrast microscopy applications.

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Lab products found in correlation

Observer d1, by Zeiss (3 mentions) 30 mm glass base, by Greiner (3 mentions) Zen 2 acquisition software, by Zeiss (3 mentions) Axiocam mrm camera, by Zeiss (3 mentions)

3 protocols using plan neofluor 0.75 na ph2 dry objective

Fluorescence Microscopy of Live Cells

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HeLa LAP2b:RFP or tubulin:GFP cells were seeded onto Petri dishes with a 30-mm glass base (Greiner), and an inverted fluorescence microscope (Observer D1; Zeiss) was used. Fluorescence or phase-contrast images were taken by using a 63× Plan Neofluor 0.75 NA Ph2 dry objective (Zeiss). Imaging was performed at 37°C in 5% CO₂ by using a Zeiss AxioCam MRm camera and Zeiss ZEN 2 acquisition software. Drugs were added to the medium immediately before filming as appropriate.

Petsalaki E, & Zachos G. (2020). An ATM–Chk2–INCENP pathway activates the abscission checkpoint. The Journal of Cell Biology, 220(2), e202008029.

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Imaging live cells using fluorescence microscopy

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BE or HeLa LAP2b-RFP cells were seeded onto Petri dishes with a 30-mm glass base (Greiner), and an inverted fluorescence microscope (Observer D1; Zeiss) was used. Fluorescence and phase-contrast images were taken by using a 63× Plan Neofluor 0.75 NA Ph2 dry objective (Zeiss; Figs. 1 E and S1 C), a 20× Plan Neofluor 0.40 NA Ph2 dry objective (Zeiss; Fig. 7, C and D), or a 10× A-plan 0.25 Ph1 dry objective (Zeiss; Fig. 7, E and F). Imaging was performed at 37°C in 5% CO₂ by using a Zeiss AxioCam MRm camera and the Zeiss ZEN 2 acquisition software. For UCN-01 or PP2 treatments in Figs. 1 and 7, the drugs were added to the medium immediately before filming.

Dandoulaki M., Petsalaki E., Sumpton D., Zanivan S, & Zachos G. (2018). Src activation by Chk1 promotes actin patch formation and prevents chromatin bridge breakage in cytokinesis. The Journal of Cell Biology, 217(9), 3071-3089.

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Fluorescent Imaging of Live Cell Dynamics

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HeLa LAP2b:RFP or HeLa tubulin:GFP cells were seeded onto Petri dishes with a 30-mm glass base (Greiner) and an inverted fluorescence microscope (Observer D1; Zeiss) was used. Fluorescence or phase-contrast images were taken by using a 63× Plan Neofluor 0.75 NA Ph2 dry objective (Zeiss). Imaging was performed at 37°C in 5% CO₂ by using a Zeiss AxioCam MRm camera and Zeiss ZEN 2 acquisition software. Drugs were added to the medium immediately before filming as appropriate.

Petsalaki E., Balafouti S., Kyriazi A.A, & Zachos G. (2023). The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knots. The Journal of Cell Biology, 222(11), e202303123.

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