At sacrifice, blood was collected from the heart, and serum was isolated after centrifugation for 10 min at 5,000 g. TAG (SB-2100-430; Stanbio Laboratory, Boerne, TX), glucose (997-03001; Wako Diagnostics, Lexington, MA), ketone bodies (415-73301; Wako Diagnostics, Lexington, MA), NEFAs (999-34691; Wako Diagnostics, Lexington, MA), and cholesterol (999-02601; Wako Diagnostics, Lexington, MA) were measured using 96-well plate formats as per the manufacturer's instructions.
Ketone bodies
Manufactured by Fujifilm
Sourced in Spain, Japan
Ketone bodies is a lab equipment product designed for the detection and measurement of ketone bodies in biological samples. Ketone bodies are a group of organic compounds that include acetone, acetoacetic acid, and beta-hydroxybutyric acid. This product provides a reliable and accurate method for analyzing and quantifying ketone bodies in various sample types.
Automatically generated - may contain errors
3 protocols using ketone bodies
1
Serum Metabolite Profiling in Mice
2
Blood Metabolic Profiling in Mice
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Mice were killed 4 h after the start of the light cycle. Whole trunk blood was collected and then spun for 15 min at 3,000g and 4 °C. Serum cholesterol, glucose, TG levels (Spinreact, Spain); FFA, ketone bodies levels (Wako, USA) and adiponectin (Millipore, USA) were assessed using commercial kits based on a colorimetric method. Serum insulin levels were measured by RIA following the manufacturerŕs instructions. Serum activities of ALT and AST were measured using the ALT and AST Reagent Kit (Biosystems, Spain) with a Benchmark Plus Microplate Spectrophotometer.
3
Lipid and Glucose Metabolism Analysis
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Plasma and tissue levels of TG, phospholipids (PL), free cholesterol (FC), and cholesteryl esters (CE) were determined by a chemical commercial kit (Weiner Lab, Rosario, Argentina). For tissue lipid levels, lipids were extracted previous to the determination by the Folch method [11] . Blood glucose was monitored using blood glucose strips and the Accu-Check glucometer (Roche Diagnostics, Vienna, Austria). Plasma insulin concentration was measured by ELISA (Millipore Corp., MA). Plasma fatty acids (FA) levels were determined using NEFA C commercial kit according to the manufacturer's protocol (Wako, Japan). Hepatic enzymes were measured using a commercial kit (Biotron Diagnostic, CA). Ketone bodies were measured according to manufacturer's instructions (Wako, Japan). Plasma β-hydroxybutyrate levels were measured via the cyclic enzymatic method using Autokit Total Ketone bodies (Wako, Japan).
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