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Alexa fluor 594 affinity pure conjugate

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Alexa Fluor 594 Affinity Pure conjugate is a fluorescent dye used for labeling and detection in biological applications. It has an excitation maximum at 590 nm and an emission maximum at 617 nm.

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Lab products found in correlation

Cy3 affinitypure donkey anti guineapig, by Jackson ImmunoResearch (2 mentions) Alexa fluor plus 488 conjugate, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 conjugate, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Alexa Fluor 594 (140 citations) Alexa 594 (38 citations)

2 protocols using alexa fluor 594 affinity pure conjugate

1

Multicolor Pancreatic Endocrine Cell Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dispersed cells from anti-NTPDase3 pull-down and flow-through
fractions were fixed and double stained for insulin and glucagon (both
fractions), insulin and somatostatin (pull-down fraction) or glucagon and
somatostatin (flow-through fraction). Primary antibodies used were guinea
pig anti-insulin (Fitzgerald #20-IP35, 1:1000), rabbit anti-glucagon (Cell
Signaling #2760, 1:200), and mouse anti-somatostatin (Santa Cruz sc-55565,
1:100). Secondary antibodies used were goat anti-rabbit Alexa Fluor 488
conjugate (Cell Signaling #4412, 1:500), Cy3 AffinityPure donkey anti-guinea
pig (Jackson ImmunoResearch #706–165–148, 1:500), goat
anti-mouse Alexa Fluor Plus 488 conjugate (Thermofisher A32723, 1:500), goat
anti-mouse Alexa Fluor 594 Affinity Pure conjugate (Jackson ImmunoResearch
#115–585–020, 1:500).
Ho T., Potapenko E., Davis D.B, & Merrins M.J. (2023). A plasma membrane-associated glycolytic metabolon is functionally coupled to KATP channels in pancreatic α and β cells from humans and mice. Cell reports, 42(4), 112394.
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2

Multicolor Pancreatic Endocrine Cell Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dispersed cells from anti-NTPDase3 pull-down and flow-through
fractions were fixed and double stained for insulin and glucagon (both
fractions), insulin and somatostatin (pull-down fraction) or glucagon and
somatostatin (flow-through fraction). Primary antibodies used were guinea
pig anti-insulin (Fitzgerald #20-IP35, 1:1000), rabbit anti-glucagon (Cell
Signaling #2760, 1:200), and mouse anti-somatostatin (Santa Cruz sc-55565,
1:100). Secondary antibodies used were goat anti-rabbit Alexa Fluor 488
conjugate (Cell Signaling #4412, 1:500), Cy3 AffinityPure donkey anti-guinea
pig (Jackson ImmunoResearch #706–165–148, 1:500), goat
anti-mouse Alexa Fluor Plus 488 conjugate (Thermofisher A32723, 1:500), goat
anti-mouse Alexa Fluor 594 Affinity Pure conjugate (Jackson ImmunoResearch
#115–585–020, 1:500).
Ho T., Potapenko E., Davis D.B, & Merrins M.J. (2023). A plasma membrane-associated glycolytic metabolon is functionally coupled to KATP channels in pancreatic α and β cells from humans and mice. Cell reports, 42(4), 112394.
+ Open protocol
+ Expand

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