RNA Immunoprecipitation assay (RIP) was conducted with the application of Z-Magna RIPTM RNA-binding Protein Immunoprecipitation kit (Millipore Corporation (Bedford, MA, USA)). Anti-ELAVL1 (Abcam) antibody and anti-IgG (Abcam (Cambridge, MA, USA)) antibody were used to immunoprecipitate cell lysates. Finally, the RNA complexes were extracted for RT-qPCR analysis. The experimental procedure was independently carried out in triplicate.
Z magna riptm rna binding protein immunoprecipitation kit
Manufactured by Merck Group
Sourced in United States
The Z-Magna RIPTM RNA-binding Protein Immunoprecipitation kit is a tool designed for the isolation and purification of RNA-binding proteins from cell or tissue samples. It utilizes magnetic beads coated with an antibody specific to the target RNA-binding protein, allowing for efficient capture and separation from the sample.
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3 protocols using z magna riptm rna binding protein immunoprecipitation kit
1
RNA-Binding Protein Immunoprecipitation Assay
2
m6A Methylation Protein Binding Assay
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RIP assay was conducted by using Z-Magna RIPTM RNA-binding Protein Immunoprecipitation kit (Millipore Corporation, USA). Cells lysates were obtained by treated cells with lysis buffer, Next, cell lysates were immunoprecipitated with anti-m6A (1mg/ml; Abcam, CA, USA), anti-METTL3 (1mg/ml; Abcam), anti-ENO1 (850μg/ml; Abcam), anti-PKM (550μg/ml; Abcam), anti-IGF2BP3 and negative control anti-IgG (1mg/ml; Abcam). Finally, the RNA complexes were extracted for RT-qPCR. The experiment was performed in triplicate.
3
Ago2 RNA-binding Protein Immunoprecipitation
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Three experiments were implemented via a Z-Magna RIPTM RNA-binding Protein Immunoprecipitation kit (Millipore Corporation, USA). Cell lysates were treated with Anti-Ago2 (Abcam) antibody and anti-IgG (Abcam) antibody, and analyzed by RT-qPCR.
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