The concentrations of H2O2 and NO2−/NO3− in the water were measured using a hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific) and a nitrite/nitrate colorimetric assay kit (Cayman), respectively. ˙OH, 1O2, ˙NO, O2˙−, ˙NO2, and ONOO− were measured using an electron spin resonance (ESR) spectroscope (Bruker) with relevant spin traps (29 (link)). The spin traps were 100 mM 5,5-dimethyl-1-pyrroline N-oxide (DMPO; Dojindo) for trapping ˙OH, 5 mM N-(dithiocarbamoyl)-N-methyl-d -glucamine (MGD; Dojindo) for trapping ˙NO, 10 mM 2,2,6,6-tetramethylpiperidine (TEMP; TCI) for trapping 1O2, and 10 mM 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine (TEMPONE-H; Enzo) for trapping O2˙−, ˙NO2, and ONOO−.
Hydrogen peroxide peroxidase assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Hydrogen peroxide/peroxidase assay kit is a laboratory product designed to quantitatively measure hydrogen peroxide and peroxidase activity in biological samples. The kit provides the necessary reagents and protocol to perform the assay.
Automatically generated - may contain errors
Lab products found in correlation
Nitrate nitrite colorimetric assay kit, by Cayman Chemical (3 mentions)
Tempone h, by Enzo Life Sciences (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
5 5 dimethyl 1 pyrroline n oxide dmpo, by Dojindo Laboratories (1 mentions)
Esr spectroscope, by Bruker (1 mentions)
Esr spectroscopy, by Bruker (1 mentions)
5 5 dimethyl 1 pyrroline n oxide, by Dojindo Laboratories (1 mentions)
Amplex red, by Thermo Fisher Scientific (1 mentions)
Infinite 200, by Tecan (1 mentions)
4 protocols using hydrogen peroxide peroxidase assay kit
1
Measuring Reactive Oxygen and Nitrogen Species
2
Quantifying Reactive Species in Saline
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The concentrations of H2O2 and / in 0.9% NaCl were measured using hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific) and nitrite/nitrate colorimetric assay kit (Cayman), respectively. •OH, 1O2, •NO, , •NO2, and ONOO− were measured using an electron spin-resonance (ESR) spectroscopy (Bruker) together with relevant spin traps. The latter include: 100 mM DMPO (5,5-Dimethyl-1-pyrroline N-oxide, Dojindo), 5 mM MGD (N-(Dithiocarbamoyl)-N-methyl-D-glucamine, Dojindo), 10 mM TEMP (2,2,6,6-Tetramethylpiperidine, TCI), and 10 mM TEMPONE-H (1-Hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine, Enzo).
3
Plasma Jet Treatment: Quantifying H2O2, NO2-, and NO3- Levels
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The concentrations of H2O2, NO2−, and NO3− after plasma jet treatment in the medium without cells were measured using the hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific) and nitrite/nitrate colorimetric assay kit (Cayman), respectively. The plasma jet setting up was the same as before, 200 μL medium in 24-well plates was treated with plasma jet for different durations, and species was measured using a microplate reader (Thermo Fisher Scientific).
4
Kinetics of H2O2 release from cells
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The kinetics of H 2 O 2 released from cells was done using Amplex® Red (10-acetyl-3, 7-dihydroxyphenoxazine) hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific, Waltham, MA, USA) as per the kit protocol with minor modifications. Briefly, spent medium was removed from the wells of 96-well culture plate after 72 hours of siRNA treatment and Krebs-Ringer phosphate (KRPG) buffer added to the cells. This was followed by 100µL of reaction mixture containing 50µM Amplex® Red reagent and 0.1U/mL HRP in KRPG. Amplex® Red reagent reacts with H 2 O 2 to produce resorufin. Fluorescence (excitation at 530-560nm and emission detection at 590nm) was measured immediately, and subsequently measured every 30 minutes until 90 minutes on Tecan Infinite 200 (Tecan, Männedorf, Switzerland).
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