S 4 nitrobenzyl 6 thioinosine nbmpr

KMS11, LP1, OCI‐AML‐2, and OCI‐AML‐3 cell lines were cultured as described previously [6]. S49 wild‐type (CCLZR352) and kin‐ (CCLZR347) cells were purchased from the University of California, San Francisco (UCSF) Cell Culture Facility and were cultured in Dulbecco's modified Eagle medium supplemented with 10% heat‐inactivated horse serum, 100 units·mL⁻¹ penicillin, and 100 μg·mL⁻¹ streptomycin. Cell lines were routinely confirmed to be mycoplasma‐free using the MycoAlert Mycoplasma Detection Kit (Lonza, Mississauga, Canada). Atorvastatin calcium (21CEC Pharmaceuticals Ltd., Markham, Canada) and fluvastatin sodium (US Biological, Burlington, Canada) were dissolved in ethanol. Dipyridamole (Sigma, Oakville, Canada), cilostazol (Tocris Bioscience, Burlington, Canada), S‐(4‐nitrobenzyl)‐6‐thioinosine (NBMPR) (Tocris Bioscience), 4‐{[3′,4′‐(methylenedioxy)benzyl]amino}‐6‐methoxyquinazoline (MBMQ) (Calbiochem, Oakville, Canada), fasentin (Sigma), and forskolin (Sigma) were dissolved in DMSO. Mevalonate and dibutyryl‐cAMP (db‐cAMP) were purchased from Sigma and dissolved in water. Geranylgeranyl pyrophosphate (GGPP) (methanol : ammonia solution) was purchased from Sigma.