Siliconized beveled glass micropipettes

Four WPI, a second surgery was performed to transplant hNSC in Rag1 mice or mT-mNSC in CRYM-ZsGreen1 transgenic mice as previously described [29 (link), 31 (link), 44 (link)]. After re-exposure of the spinal cord at C5, cells were injected using a NanoInjector with micromanipulator (World Precision Instruments, Sarasota, FL) and siliconized beveled glass micropipettes (outer diameter = 100–110 mm; inner diameter = 70 mm; Sutter Instruments, Novato, CA). A total of 75,000 cells or vehicle (X-VIVO media) in 1uL total volume was delivered to the spared spinal parenchyma at four sites, two rostral and two caudal to the lesion, 250nL per site (schematic in Fig. 1C). All mice received post-operative care as described above.

Four WPI, a second surgery was performed to transplant hNSC in Rag1 mice or mT-mNSC in CRYM-ZsGreen1 transgenic mice as previously described^{29 (link),31 (link),44 (link)}. After re-exposure of the spinal cord at C5, cells were injected using a NanoInjector with a micromanipulator (World Precision Instruments, Sarasota, FL) and siliconized beveled glass micropipettes (outer diameter = 100–110 mm; inner diameter = 70 mm; Sutter Instruments, Novato, CA). A total of 75,000 cells or vehicle (X-VIVO media) in 1 uL total volume was delivered to the spared spinal parenchyma at four sites, two rostral and two caudal to the lesion, 250 nL per site (schematic in Fig. 1c). All mice received postoperative care as described above.