Nifedipine was kindly donated by Eipico (Cairo, Egypt). Poly(D,L-lactide-co-glycolide) (50:50; molecular weight 85,000 Da), polyvinyl alcohol (molecular weight 12,000–13,000 Da and 87–89% degree of hydrolysis), chitosan (low molecular weight 150,000 Da and 75–85% degree of acetylation), atorvastatin, sodium carboxymethyl cellulose, Tween 80 (polyoxyethylene sorbitan monooleate), ammonium acetate, acetonitrile (HPLC grade) and ethyl acetate (HPLC grade) were procured from Sigma-Aldrich (St. Louis, MO, USA). Dialysis bags with a molecular weight cut off of 12,000 Da were purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). Bleomycin injection was obtained from Cipla (Mumbai, India). All other enrolled ingredients were of analytical grade.
Tween 80 polyoxyethylene sorbitan monooleate
Manufactured by Merck Group
Sourced in Germany, United States
Tween 80 (polyoxyethylene sorbitan monooleate) is a non-ionic surfactant commonly used in pharmaceutical and laboratory applications. It is a water-soluble, emulsifying agent that can help solubilize or disperse hydrophobic compounds in aqueous systems.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade acetonitrile, by Merck Group (2 mentions)
Glycerol, by Merck Group (2 mentions)
Soybean oil, by Merck Group (2 mentions)
Sunflower oil, by Merck Group (2 mentions)
Cremophor el, by Merck Group (2 mentions)
Propylene glycol, by Merck Group (2 mentions)
Dialysis bag, by Serva Electrophoresis (1 mentions)
Ethyl acetate hplc grade, by Merck Group (1 mentions)
Atorvastatin, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Sodium carboxymethyl cellulose, by Merck Group (1 mentions)
Na2so4, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
Squaric acid, by Merck Group (1 mentions)
Ethylene brassylate, by Merck Group (1 mentions)
Methanol, by Avantor (1 mentions)
Formic acid, by Avantor (1 mentions)
14 protocols using tween 80 polyoxyethylene sorbitan monooleate
1
Nifedipine and PLGA Nanoparticle Formulation
2
Optimizing Zebrafish Oil Spill Remediation
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Tween-80
(polyoxyethylene
sorbitan monooleate, ≥99%) was obtained from Sigma-Aldrich
(Waltham, MA). 1-Butyl-3-methylimidazolium lauroyl sarcosinate [Bmim][Lausar]
was prepared using a metathesis reaction established by Mustahil et
al.19 (link) Dichloromethane (DCM), NaCl, MgCl2, and Na2SO4 (purity > 98%) were
obtained
from Merck (Germany). Zebrafish (D. rerio) were purchased from an aquatic store in Seri Iskandar, Malaysia.
Arab crude oil with a density of 902 g L–1 and a
viscosity of 5.34 × 10–5 m2 s–1 at room temperature was acquired from the Petroleum
Industry of Malaysia Mutual Aid Group Sdn Bhd (PETRONAS). Stimulated
seawater samples were prepared by an established method.12 (link) Details of the procedure are given inTable S5 .
(polyoxyethylene
sorbitan monooleate, ≥99%) was obtained from Sigma-Aldrich
(Waltham, MA). 1-Butyl-3-methylimidazolium lauroyl sarcosinate [Bmim][Lausar]
was prepared using a metathesis reaction established by Mustahil et
al.19 (link) Dichloromethane (DCM), NaCl, MgCl2, and Na2SO4 (purity > 98%) were
obtained
from Merck (Germany). Zebrafish (D. rerio) were purchased from an aquatic store in Seri Iskandar, Malaysia.
Arab crude oil with a density of 902 g L–1 and a
viscosity of 5.34 × 10–5 m2 s–1 at room temperature was acquired from the Petroleum
Industry of Malaysia Mutual Aid Group Sdn Bhd (PETRONAS). Stimulated
seawater samples were prepared by an established method.12 (link) Details of the procedure are given in
3
Synthesis and Characterization of Fluorescent Nanostructures
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All the reagents used were of analytical purity and employed without further purification: ethylene brassylate (EB, 1,4-dioxacycloheptadecane-5,17-dione, C15H26O4, Mw = 270.36 g/mol, purity (GC) > 95.0%), squaric acid (SA, 3,4-dihydroxy-3-cyclobutene-1,2-dione, H2C4O4, Mw = 114.06 g/mol, purity (HPLC) > 99.0%), norfloxacin (NRF, 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, C16H18 FN3O, Mw = 319.33), dimethylsulfoxid (DMSO, (CH3)2SO, Mw = 78.13 g/mol) and Tween 80 (polyoxyethylenesorbitan monooleate) were purchased from Sigma-Aldrich Co., (Burlington, MA, USA), 1-hexanol anhydrous from Across-Organics (Geel, Belgium), Brij 58 (polyoxyethylene (20) cetylether, HO-(CH2CH2O)n-(CH2)15-CH3) from Merck (Hohenbrunn, Germany), while hydrochloric acid (HCl, Mw 36.5) was acquired from Chimreactiv (Bucuresti, Romania). The ultrapure water used in the experiments was prepared with a Milli-Q device.
4
Formulation and Characterization of Propofol Nanoemulsions
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Propofol (purity 99.9%) was obtained from Incepta Pharmaceuticals Ltd. (Dhaka, Bangladesh). Zanthoxylum rhetsa seed oil (ZRO) was collected by a steam distillation process from naturally obtained seeds (no organic solvent was used). Medium-chain triglycerides (carbon chain length 8–12), Miglyol 810 (M810), and corn oil (CO) were purchased from Sasol Germany GmbH (Werk, Witten, Germany). Capmul MCM (C10) mono/diglycerides of capric acid were purchased from Abitec Corp. Janesville, USA. Maisine 35–1 (long-chain monoglycerides) was obtained from Gattefosse SAS, (Saint-Priest, France). Kolliphor ELP and Kolliphor HS15 were purchased from BASF, Ludwigshafen, Germany. Tween 80 (polyoxyethylene sorbitan monooleate) was obtained from Sigma Aldrich, USA. HPLC-grade acetonitrile, methanol, formic acid, and phosphoric acid were obtained from BDH Chemicals Ltd., Poole, UK. Milli-Q water was obtained through a Milli-Q Integral Water Purification System (Millipore, Bedford, MA). All other reagents were of analytical grade and used without further purification.
5
Preparation of BCG-CWS and WT1 Peptide Emulsion
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We prepared the BCG-CWS as an oil-in-water emulsion product according to a previous report.[19 (link),35 ] Briefly, 2 mg of BCG-CWS and 20 μl of Drakeol 6VR light mineral oil (Penreco, Karns City, PA) were gently mixed and homogenized for 1 minute. Then, the BCG-CWS/Drakeol solution was homogenized for 4 minutes with 1 ml of emulsion buffer (1% Tween 80 [polyoxyethylene sorbitan monooleate; Sigma, St Louis, MO] diluted by saline [Otsuka Pharmaceutical, Tokyo, Japan]). The BCG-CWS solution was then incubated at 60°C for 30 minutes. The final concentration of BCG-CWS in the oil-in-water emulsion was 1 mg/ml.
The sequence of HLA-A∗24:02–restricted modified 9mer WT1235 peptide (mp235) was CYTWNQMNL. Good manufacturing practice (GMP) –grade mp235 was synthesized by Peptide Institute (Osaka, Japan). For preparation of WT1 peptide solution, 3 mg of mp235 was dissolved in 100 μl of dimethyl sulfoxide (DMSO; Sigma) and then was diluted with 1400 μl of 5% glucose (Otsuka Pharmaceutical). The final concentration of WT1 peptide solution was 2 mg/ml. A volume of 250 μl peptide solution (0.5 mg of mp235) was administered to the patient.
The sequence of HLA-A∗24:02–restricted modified 9mer WT1235 peptide (mp235) was CYTWNQMNL. Good manufacturing practice (GMP) –grade mp235 was synthesized by Peptide Institute (Osaka, Japan). For preparation of WT1 peptide solution, 3 mg of mp235 was dissolved in 100 μl of dimethyl sulfoxide (DMSO; Sigma) and then was diluted with 1400 μl of 5% glucose (Otsuka Pharmaceutical). The final concentration of WT1 peptide solution was 2 mg/ml. A volume of 250 μl peptide solution (0.5 mg of mp235) was administered to the patient.
6
Edible Fruit Coatings: Formulation and Application
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Sodium alginate (Manugel GHB, FMC Biopolymer Co., Philadelphia, PA, USA), glycerol (Sigma–Aldrich Chemie GmbH, Steinheim, Germany), sunflower oil (Rewe Bio, Rewe Markt Gmbh, Köln, Germany), tween 80 (polyoxyethylenesorbitan monooleate) (Sigma–Aldrich Chemie GmbH, Steinheim, Germany), span 80 (sorbitan monooleate) (Sigma–Aldrich Chemie GmbH, Steinheim, Germany), calcium L-lactate hydrate (Sigma–Aldrich Chemie GmbH, Steinheim, Germany) were used in coating formulations.
Whole cantaloupe (Cucumis melo var. cantalupensis) and strawberries (Fragaria × ananassa D.) were purchased from Krohns Obst-Gemüse-Express (Berlin, Germany), Schweiger Obst-und Gemüsehandel (Freising, Germany), and a local market in Freising (Germany). Samples were transported directly to the laboratory.
Whole cantaloupe (Cucumis melo var. cantalupensis) and strawberries (Fragaria × ananassa D.) were purchased from Krohns Obst-Gemüse-Express (Berlin, Germany), Schweiger Obst-und Gemüsehandel (Freising, Germany), and a local market in Freising (Germany). Samples were transported directly to the laboratory.
7
Fungal Conidia Isolation and Enumeration
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Fungal isolates were cultured on PDA (Oxoid Ltd., Basingstoke, UK) for 14 days at 25 ± 1 °C under 14:10 h photoperiods. Conidia were harvested by scraping the surface of the medium using sterile wooden spatula into 20 mL 0.05% (v/v) Tween 80 (polyoxyethylene sorbitan monooleate, Sigma Chemical Co., St. Louis, MO, USA) inside sterile 50 mL centrifuge tubes and homogenized by vortexing for 3 min. The conidial suspension was sieved through four layers of cheese cloth [20 (link)]. A ten-fold serial dilution of the spore suspension was done, and an aliquot estimated on a hemocytometer followed by a final adjustment to 108. For the multiple-dose bioassay, five different conidial concentrations (1 × 104 to 1 × 108 conidia mL−1), were prepared.
8
Pomegranate Fruit Compound Extraction
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Pomegranate fruits (cv. Wonderful) were collected at the ripening stage (April 2017) from a commercial farm located in Vallenar (28°SL), in the Atacama Region of Chile. Fruit samples were stored at 4 °C and processed within 24 h of collection. Orange oil (density 843 kg/m3 at 25 °C), castor oil (density 836 kg/m3 at 25 °C), Miglyol® 812 (density 945 kg/m3 at 20 °C), sunflower oil (density 919.3 kg/m3 at 25 °C) and soybean oil (density 850 kg/m3 at 25 °C) were supplied by Sigma–Aldrich Co. (Darmstadt, Germany). Polyglycerol polyricinoleate (PGPR, C21H42O6) was purchased from Brenntag AG (Essen, Germany) and used as a lipophilic emulsifier. Tween 80 (polyoxyethylene sorbitan monooleate), supplied by Sigma–Aldrich Co. (Germany), was used as hydrophilic emulsifier. HPLC-grade acetonitrile, methanol and chloroform were obtained from Sigma–Aldrich (St. Louis, MO, USA).
9
Gemcitabine Lipid Nanocarrier Synthesis
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Miglyol ® 812N oil -medium chain triglyceride (MCT) was kindly donated by CREMER OLEO GmbH & Co.
Surfactants: Kolliphor ® HS 15 (PEG-660-12 hydroxystearate) was kindly provided by BASF ChemTrade GmbH; Tween ® 80-polyoxyethylene sorbitan monooleate and hexadecyltrimethylammonium bromide (CTAB) were purchased from Sigma. Polymers: Sodium hyaluronate 50 kDa (HA, LifeCore biomedical); poly-L-glutamic acid sodium salt 5 kDa -(PGA, Sigma). Drug GEM hydrochloride (GEM-HCl) was purchased from Sigma. N4-myristoyl gemcitabine (GEM C14) and low molecular weight gelator: N4octanoyl-2ʹ-deoxycytidine were synthesised as previously reported (KJ. Skilling et al., 2018; (link)K.J. Skilling et al., 2016) (link). Fluorescent dye 9-diethylamino-5H-benzo[a]phenoxazine-5-one -(Nile Red) was purchased from MP Biomedical.
Surfactants: Kolliphor ® HS 15 (PEG-660-12 hydroxystearate) was kindly provided by BASF ChemTrade GmbH; Tween ® 80-polyoxyethylene sorbitan monooleate and hexadecyltrimethylammonium bromide (CTAB) were purchased from Sigma. Polymers: Sodium hyaluronate 50 kDa (HA, LifeCore biomedical); poly-L-glutamic acid sodium salt 5 kDa -(PGA, Sigma). Drug GEM hydrochloride (GEM-HCl) was purchased from Sigma. N4-myristoyl gemcitabine (GEM C14) and low molecular weight gelator: N4octanoyl-2ʹ-deoxycytidine were synthesised as previously reported (KJ. Skilling et al., 2018; (link)K.J. Skilling et al., 2016) (link). Fluorescent dye 9-diethylamino-5H-benzo[a]phenoxazine-5-one -(Nile Red) was purchased from MP Biomedical.
10
Formulation and Characterization of Cefuroxime Nanocarriers
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Cefuroxime, (6R,7R)-3-{[(aminocarbonyl)oxy]methyl}-7-{[(2Z)-2-(2-furyl)-2-(methoxyimino)acetyl]amino}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, was purchased from Euroasias Chemical Private Limited (Mumbai, India). Safflower seed oil, soybean oil, sunflower oil, pine nut oil, olive oil, Cremophor EL, and sodium oleate were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Lecithin Lipoid S75 was also purchased from Lipoid GmbH (Ludwigshafen, Germany); Tween 80 (polyoxyethylene sorbitan monooleate) was purchased from Merck (Hohenbrunn, Germany), and glycerol was purchased from J.T. Baker (Phillipsburg, NJ, USA). All the used oils and surfactants were analytically graded materials. Acetonitrile (high-performance liquid chromatographic [HPLC] grade, 99.9% purity) was purchased from J.T. Baker. Sodium hydroxide (analytical grade) was purchased from Merck Millipore (Billerica, MA, USA). Water was deionized and Milli-Q filtered. Centrisart tubes and dialysis membranes were from Sartorius AG (Göttingen, Germany). All other chemicals and reagents were of analytical or HPLC grade.
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