Experiments were performed using 16 BTC cell lines. Thirteen cell lines were previously established from Japanese patients with BTC15 (link), 16 (link), 17 (link): NCC‐BD1, NCC‐BD2, NCC‐BD3, NCC‐BD4‐1, and NCC‐BD4‐2 were derived from ECC, and NCC‐CC1, NCC‐CC3‐1, NCC‐CC3‐2, NCC‐CC4‐1, NCC‐CC4‐2, NCC‐CC5, NCC‐CC6‐1, and NCC‐CC6‐2 were derived from ICC. Moreover, we used three BTC cell lines (TKKK, OZ, and HuCCT1) purchased from Riken BioResource Center or from the Japanese Collection of Research Bioresources. OZ and HuCCT1 were derived from ECC, whereas TKKK was derived from ICC. TKKK and OZ cells were cultivated in DMEM plus 10% FBS and antibiotics, whereas the remaining 14 cell lines were cultivated in RPMI‐1640 medium plus 10% FBS and antibiotics. NCC‐BD2, NCC‐BD3, NCC‐BD4‐1, NCC‐CC1, NCC‐CC3‐1, NCC‐CC6‐1, and TKKK cells were cultured on collagen I‐coated dishes. The remaining nine cell lines were cultivated on normal culture dishes. All cells were kept at 37℃ in an atmosphere of 5% CO2.
Hucct1
Manufactured by RIKEN BioResource Center
Sourced in Japan
HuCCT1 is a cell line derived from a human cholangiocarcinoma, a type of liver cancer. It is a well-established model used in cancer research to study the biology and treatment of cholangiocarcinoma. The cell line is available for purchase from the RIKEN BioResource Center.
Automatically generated - may contain errors
Lab products found in correlation
Tfk 1, by RIKEN BioResource Center (10 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Rpmi 1640, by Thermo Fisher Scientific (5 mentions)
Snu245, by Korean Cell Line Bank (4 mentions)
Snu478, by Korean Cell Line Bank (4 mentions)
Snu1196, by Korean Cell Line Bank (4 mentions)
Snu 308, by Korean Cell Line Bank (4 mentions)
Snu869, by Korean Cell Line Bank (4 mentions)
Snu1079, by Korean Cell Line Bank (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Huh 28, by RIKEN BioResource Center (2 mentions)
Egi 1, by RIKEN BioResource Center (2 mentions)
Mcl 1, by Cell Signaling Technology (2 mentions)
Hcc cell lines, by American Type Culture Collection (1 mentions)
Recombinant human tgf β, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Cisplatin, by Nippon Kayaku (1 mentions)
21 protocols using hucct1
1
Characterization of Biliary Tract Cancer Cell Lines
2
Culturing Cholangiocarcinoma Cell Lines
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Two human cholangiocarcinoma cell lines (RBE and HUCCT1) were purchased from RIKEN Bioresource Center (Tsukuba, Ibaraki, Japan) and cultured in RPMI-1640 medium containing 10% fetal bovine serum in a 5% CO2 incubator at 37 °C.
3
Human Cholangiocarcinoma Cell Culture
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HuCCT1 (RCB‐1960) and Huh28 (RCB‐1943) CCA cell lines were purchased at the RIKEN BioResource Center (Tsukuba‐shi, Japan). Cells were grown in Roswell Park Memorial Institute 1640 medium (HuCCT1) or minimal essential medium (Huh28) supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum. HCC cell lines were purchased from ATCC and cultured as described.15 LX2 cells were grown as described.16 Freshly isolated human hepatocytes were purchased from Biopredic International (St. Grégoire, France). Cells were cultured at 37 °C in a 5% CO2 atmosphere, and overnight serum starvation was performed before each treatment. Unless otherwise specified, cells were treated with 1 ng/mL recombinant human TGFβ (R&D Systems, Minneapolis, MN) and 10 µM TGFβ inhibitors (Sigma‐Aldrich, St. Louis, MO).
4
Culturing Human Colorectal Cancer Cell Lines
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RBE, HuCCT1, and TFK‐1 human CC cell lines were purchased from RIKEN BioResource Center (Tsukuba, Japan) and cultured in RPMI‐1640 medium (Sigma‐Aldrich, St. Louis, MO, USA) supplemented with 10% heat‐inactivated FBS (Sigma‐Aldrich) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) at 37°C and 5% CO2. Cisplatin was purchased from Nippon Kayaku (Tokyo, Japan) and diluted with cell culture medium before use in in vitro experiments.
5
BTC Cell Lines and Chemotherapeutics
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Nine human BTC cell lines were utilized in this research. The SNU245, SNU308, SNU478, SNU869, SNU1079, and SNU1196 cell lines were purchased from Korean Cell Line Bank (Seoul, Korea). The HuCCT-1 and TFK-1 cell lines were obtained from RIKEN BioResource Center (Ibaraki, Japan). A patient-derived cell line, SNU2670, was successfully established [19 (link)]. All cells were cultured in RPMI-1640 medium (Welgene Inc., Gyeongsan, Korea) containing 10% fetal bovine serum and 10 μg/mL gentamicin at 37°C incubator under 5% CO2. The ATR inhibitor AZD6738 was kindly provided by AstraZeneca (Macclesfield, Cheshire, UK). Gemcitabine, cisplatin, and 5-fluorouracil (5-FU) were purchased from Lilly Korea Co. (Seoul, Korea), JW Pharmaceutical Co. (Seoul, Korea), and Ildong Pharmaceutical Co. (Seoul, Korea), respectively.
6
Establishment and Characterization of BTC Cell Lines
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Nine human BTC cell lines were utilized in this study. SNU245, SNU308, SNU478, SNU869, and SNU1196 cells were purchased from Korean Cell Line Bank (Seoul, Korea). HuCCT-1 and TFK-1 cells were obtained from RIKEN BioResource Center (Ibaraki, Japan). The patient-derived cell lines SNU2670 and SNU2773 were successfully established as described previously [10 (link)]. All cells were cultured in RPMI1640 medium (Welgen Inc., Gyeongsan, Korea) containing 10% fetal bovine serum and 10 μg/mL gentamicin at 37°C under 5% CO2. WEE1 (AZD1775), ATR (AZD6738), and ATM (AZD0156) inhibitors were kindly provided by AstraZeneca (Macclesfield, Cheshire, UK).
7
HuCCT1 Cell Line Culture
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The human CCA cell line HuCCT1 (Riken Bioresource Research Center, Japan) was grown in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a 5% CO2 humidified atmosphere. For signal transduction experiments, cells were starved overnight in a serum-free medium.
8
Cholangiocarcinoma Cell Line Culture
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The human cholangiocarcinoma cell lines HuCCT-1, HCCC-9810 and RBE were purchased from the RIKEN bioresource Center (Saitama-ken, Japan). The TFK-1 cell line was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany). Cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640, Thermo Fisher Scientific, New York, USA) medium with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific) at 37°C in a humidified atmosphere comprising 5% CO2. All cell lines were tested for mycoplasma contamination (Genecreate Biological engineering Co., Ltd, Wuhan, China).
9
Establishing and Characterizing Normal Human Cholangiocytes
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Primary cultures of normal human cholangiocytes (NHC) were established, characterized and cultured as previously described (Urribarri et al, 2014 (link)). HuCCT1 and Huh28 CCA cell lines were purchased from RIKEN BioResource Center (Tsukuba-shi, Japan) and CCLP1, Egi-1, SG231, TFK1, Sk-ChA-1 and Mz-ChA-1 were provided by Laura Fouassier (Paris, France). HepG2/C3A and HEK293T were purchased from ATCC (www.lgcstandards-atcc.org ). Serum-starved cells were stimulated with the indicated concentrations of human recombinant TGFβ1 (R&D Systems) and for the indicated time periods as shown in the figures and figure legends.
10
Culturing Human CCA Cell Lines
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Human CCA cell lines HuCCT1 and TFK-1 were purchased from the RIKEN BRC (Ibaraki, Japan). Cells were cultured in RPMI-1640 (Gibco, NY) with 10 % fetal bovine serum (FBS) (Gibco, NY) and 100 U/mL penicillin and 100 µg/mL streptomycin (Invitrogen, MA) and incubated at 37 °C in a 5 % CO2 humidified atmosphere.
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