Dulbecco s modified eagle medium nutrient mixture f 12 medium

Human EC cell lines including HEC1A, RL952, AN3CA were bought from American Type Culture Collection (ATCC, USA), and human EC cell line Ishikawa was purchased from BANCO DE CÉLULAS DO RIO JANEIRO (BCRJ, Brazil). Normal human endometrial (uterine) epithelial cells (HEEC) were purchased from Lifeline® Cell Technology (Lifeline® Cell Technology, USA). Immortalized human endometrial stromal cell (CRL-4003) were purchased from American Type Culture Collection (ATCC, USA). The HEC1A cells were cultured in medium containing 90% McCoy’s 5A (modified) Medium (Gibco, USA) and 10% fetal bovine serum (FBS) (Gibco, USA). The Ishikawa cells were cultured in medium containing 90% Dulbecco’s Modified Eagle Medium (Gibco, USA) and 10% FBS (Gibco, USA). The RL952 cells were cultured in medium containing 90% Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Medium (Gibco, USA) and 10% FBS (Gibco, USA). The AN3CA cells were cultured in medium containing 90% Minimum Essential Medium (Gibco, USA) and 10% FBS (Gibco, USA). The HEEC cells were cultured in Lifeline® ReproLife™ Medium (Lifeline® Cell Technology, USA). All cells were cultured at 37°C in a humidified incubator with 5% CO₂ atmosphere. The SIRT2 expression in human EC cells and HEEC cells were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot.

Dulbecco's modified Eagle medium/nutrient mixture F12 medium, fetal bovine serum, PBS solution, 0.25% trypsin EDTA solution, and penicillin/streptomycin solution were purchased from Gibco BRL (Grand Island, NY). We purchased 4ʹ,6-Diamidino-2-phenylindole (#C1006) was purchased from Beyotime Biotechnology (Shanghai, China). The antibodies used in this study are listed in Supplementary Table S1 and S2.