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Envision system hrp rabbit antibody

Manufactured by Agilent Technologies
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The EnVision System–HRP-Rabbit antibody is a laboratory equipment product designed for immunohistochemical (IHC) and immunocytochemical (ICC) applications. It serves as a detection system, facilitating the visualization of target antigens in biological samples using horseradish peroxidase (HRP) and a rabbit-derived antibody.

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Lab products found in correlation

Tyramide signal amplification system, by PerkinElmer (4 mentions) Cy 3 affinipure donkey anti mouse igg h l, by Jackson ImmunoResearch (2 mentions) Hpa035977, by Merck Group (2 mentions) Clone gi191e a8, by Cell Marque (2 mentions) Biotinylated pna, by Vector Laboratories (1 mentions) Nis elements software, by Nikon (1 mentions)

5 protocols using envision system hrp rabbit antibody

1

Immunohistochemical Analysis of KMT2D in DLBCL

Cited 2 times
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DLBCL tissue microarrays (TMA) were constructed according to standard procedures58 (link) and analyzed for KMT2D expression by IHC, using a rabbit polyclonal antibody directed against the C-terminus of the KMT2D protein (HPA035977, Sigma-Aldrich) (1:200 dilution). Cases were independently scored by two pathologists (D.D-S. and S.H.), and were considered positive if ≥30% tumor cells showed staining in the nucleus. Double-immunofluorescence analysis of KMT2D and BCL6 was performed on formalin-fixed paraffin embedded (FFPE) material from reactive human tonsils using the above-mentioned anti-KMT2D antibody (1:200 dilution) and a mouse monoclonal anti-BCL6 antibody (1:300 dilution)(clone GI191E/A8, Cell Marque). Detection of KMT2D was obtained using the EnVision System-HRP-Rabbit antibody (Dako) followed by Tyramide Signal Amplification system (PerkinElmer); the secondary antibody for BCL6 was Cy-3 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:400 dilution) (Jackson ImmunoResearch Laboratories, cat# 715-167-003), as reported59 (link).
Zhang J., Dominguez-Sola D., Hussein S., Lee J.E., Holmes A.B., Bansal M., Vlasevska S., Mo T., Tang H., Basso K., Ge K., Dalla-Favera R, & Pasqualucci L. (2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nature medicine, 21(10), 1190-1198.
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2

Dual Detection of CREBBP and PNA in Mouse Spleen

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Double-immunofluorescence analysis of CREBBP and PNA was performed on
formalin-fixed paraffin-embedded (FFPE) material from mouse spleens, using a
combination of two anti-CREBBP antibody (each at 1:400 dilution)(A22 and C20,
Santa Cruz Biotechnologies) and biotinylated PNA (1:300 dilution)(Vector
Laboratories, cat#B-1075). A serial section was stained with anti-EP300
(1:200 dilution)(N15, Santa Cruz Biotechnologies). Detection of CREBBP and EP300
was obtained using the EnVision System–HRP-Rabbit antibody (Dako)
followed by Tyramide Signal Amplification system (PerkinElmer);
NeutrAvidin®, FITC conjugate (cat#A2662, Invitrogen) was used at
1:300 dilution to detect PNA.
Zhang J., Vlasevska S., Wells V.A., Nataraj S., Holmes A.B., Duval R., Meyer S.N., Mo T., Basso K., Brindle P.K., Hussein S., Dalla-Favera R, & Pasqualucci L. (2017). The Crebbp acetyltransferase is a haploinsufficient tumor suppressor in B cell lymphoma. Cancer discovery, 7(3), 322-337.
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3

Dual Immunofluorescence Analysis of Transcriptional Regulators

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Double-immunofluorescence analysis of Crebbp or Ep300 and PNA was
performed on formalin-fixed paraffin-embedded (FFPE) material from mouse
spleens. Serial sections (3mm-thick) were stained using a combination of two
anti-CREBBP antibody (each at 1:400 dilution) (A22 and C20, rabbit
polyclonal, Santa Cruz Biotechnologies) or an anti-EP300 antibody (1:400)
(N15, rabbit polyclonal, Santa Cruz Biotechnologies) and biotinylated PNA
(1:300 dilution) (Vector Laboratories, cat#B-1075). Detection of CREBBP and
EP300 was obtained using the EnVision System–HRP-Rabbit antibody
(Dako) followed by Tyramide Signal Amplification system (PerkinElmer);
NeutrAvidin®, FITC conjugated (cat#A2662, Invitrogen) was used at
1:300 dilution to detect PNA. Images were captured using a Nikon Eclipse
microscope and the NIS Elements software (Nikon). All images were colored,
resized, and merged using Adobe Photoshop (version 10.0).
Meyer S.N., Scuoppo C., Vlasevska S., Bal E., Holmes A.B., Holloman M., Garcia-Ibanez L., Nataraj S., Duval R., Vantrimpont T., Basso K., Brooks N., Dalla-Favera R, & Pasqualucci L. (2019). Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Immunity, 51(3), 535-547.e9.
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4

Histopathological Analysis of Xenografts

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Paraffin sections from multiple biopsies and sections of explanted xenografts were stained with haematoxylin and eosin for light microscopy. Sections were analysed semi-quantitatively for the presence of haemorrhage, necrosis, thrombosis and cellular infiltrates. Frozen sections of donor pig heart biopsy were immunostained with mouse anti-CD141 (clone PBS-0; 1:2,000, Abcam, Cambridge, MA, USA) or mouse IgG1 antibody (1:200, Abcam). Detection of CD141 was done using the EnVision System–HRP-Rabbit antibody (Cat# K4008; Dako, Carpinteria, CA, USA) followed by DAB+ Chromogen.
Mohiuddin M.M., Singh A.K., Corcoran P.C., Thomas III M.L., Clark T., Lewis B.G., Hoyt R.F., Eckhaus M., Pierson III R.N., Belli A.J., Wolf E., Klymiuk N., Phelps C., Reimann K.A., Ayares D, & Horvath K.A. (2016). Chimeric 2C10R4 anti-CD40 antibody therapy is critical for long-term survival of GTKO.hCD46.hTBM pig-to-primate cardiac xenograft. Nature Communications, 7, 11138.
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5

Immunohistochemical Analysis of KMT2D in DLBCL

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
DLBCL tissue microarrays (TMA) were constructed according to standard procedures58 (link) and analyzed for KMT2D expression by IHC, using a rabbit polyclonal antibody directed against the C-terminus of the KMT2D protein (HPA035977, Sigma-Aldrich) (1:200 dilution). Cases were independently scored by two pathologists (D.D-S. and S.H.), and were considered positive if ≥30% tumor cells showed staining in the nucleus. Double-immunofluorescence analysis of KMT2D and BCL6 was performed on formalin-fixed paraffin embedded (FFPE) material from reactive human tonsils using the above-mentioned anti-KMT2D antibody (1:200 dilution) and a mouse monoclonal anti-BCL6 antibody (1:300 dilution)(clone GI191E/A8, Cell Marque). Detection of KMT2D was obtained using the EnVision System-HRP-Rabbit antibody (Dako) followed by Tyramide Signal Amplification system (PerkinElmer); the secondary antibody for BCL6 was Cy-3 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:400 dilution) (Jackson ImmunoResearch Laboratories, cat# 715-167-003), as reported59 (link).
Zhang J., Dominguez-Sola D., Hussein S., Lee J.E., Holmes A.B., Bansal M., Vlasevska S., Mo T., Tang H., Basso K., Ge K., Dalla-Favera R, & Pasqualucci L. (2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nature medicine, 21(10), 1190-1198.
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