Cells were plated in 6-well plates and incubated for 48 h after transfection. The tandem fluorescent mRFP-GFP-LC3 plasmid was obtained from GenePharma Biotechnology (Suzhou, China). Autophagic changes were observed under a fluorescence microscope (E3I-630, MOTIC, USA) that visualized 200x images. The numbers of red fluorescent protein (RFP) puncta (red puncta) were used to evaluate the activity of autophagy.
Mrfp gfp lc3 plasmid
Manufactured by GenePharma
Sourced in China
The MRFP-GFP-LC3 plasmid is a laboratory tool used for the study of autophagy. It contains a fusion of monomeric red fluorescent protein (mRFP), green fluorescent protein (GFP), and the microtubule-associated protein 1 light chain 3 (LC3) gene. This plasmid is commonly used as a marker to visualize and track the process of autophagy in living cells.
Automatically generated - may contain errors
Lab products found in correlation
Tcs sp5, by Leica (1 mentions)
Ab108327, by Abcam (1 mentions)
Ab48394, by Abcam (1 mentions)
Rhodamine 123, by Beyotime (1 mentions)
Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions)
D limonene, by Merck Group (1 mentions)
Antibody against β actin a1978, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Matrigel, by Corning (1 mentions)
3 protocols using mrfp gfp lc3 plasmid
1
Quantifying Autophagy via Fluorescent Puncta
2
Autophagic Flux Analysis with mRFP-GFP-LC3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For autophagic flux analysis, cells were transfected with the mRFP-GFP-LC3 plasmid (GenePharma, Shanghai, China). Cells were then seeded into glass dishes for 72 h. The accumulation and distribution of mRFP-GFP-LC3 or mRFP-LC3 puncta were observed and captured by a Leica laser scanning confocal microscope (Leica, TCS SP5, Japan).
3
D-limonene Apoptosis and Autophagy Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
d -limonene was purchased from Sigma-Aldrich (St Louis, MO, USA). Antibody against β-actin (A1978) was purchased from Sigma-Aldrich, and antibodies against BAX (32503), cleaved PARP (32064), LC3B (ab48394), and Atg5 (ab108327) were purchased from Abcam (Cambridge, UK). Small-interfering RNA (siRNA) against atg5 and the mRFP-GFP-LC3 plasmid were obtained from GenePharma (Shanghai, China). The BD FITC Annexin V Apoptosis Detection Kit I was obtained from BD Biosciences (San Jose, CA, USA). The Cell Counting Kit-8 was purchased from Dojindo Laboratories (Kumamoto, Japan). Matrigel® was purchased from Corning (Coring, NY, USA). BALB/c mice were obtained from Shanghai SLAC Laboratory Animal Co. (Shanghai, China). Rhodamine 123 was purchased from Beyotime (Shanghai, China).
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!