Plasma D-lactate and proinflammatory cytokines were quantized using enzyme-linked immunosorbent assays (ELISA). EnzyChrom™ D-Lactate Assay Kit was purchased from BioAssay Systems (Hayward, CA). Rat IL-1 beta ELISA Kit (ab255730), IL-6 ELISA Kit (ab234570) and TNF alpha ELISA Kit (ab236712) were purchased from Abcam (Shanghai, China). Establishment of standard curves and specific experimental steps followed the instructions of above ELISA kits. The optical density (OD) values of D-Lactate and cytokines (IL-1β, IL-6 and TNF-α) were respectively read at 565 and 450 nm.
Tnf alpha elisa kit
Manufactured by Abcam
Sourced in United States, China
The TNF alpha ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human tumor necrosis factor alpha (TNF-α) in cell culture supernatants, serum, and plasma samples. The kit utilizes a specific antibody coated on a 96-well plate to capture TNF-α, and a detection antibody conjugated with an enzyme to quantify the captured TNF-α through a colorimetric reaction.
Automatically generated - may contain errors
Lab products found in correlation
Il 6 elisa kit, by Abcam (2 mentions)
Aspartate aminotransferase activity, by Abcam (2 mentions)
Bromocriptine, by Novartis (2 mentions)
Creatinine, by Abcam (2 mentions)
Alloxan, by Merck Group (2 mentions)
Alkaline phosphatase, by Abcam (2 mentions)
Urea, by Abcam (2 mentions)
Ldh 1 assay kits, by Abcam (2 mentions)
2 3 5 triphenyltetrazolium chloride, by GoldBio (2 mentions)
Rat il 1 beta elisa kit, by Abcam (1 mentions)
Il 1β elisa kit, by Abcam (1 mentions)
Cabergoline, by Pfizer (1 mentions)
Spectramax m5 microplate reader, by Molecular Devices (1 mentions)
Mouse il 6 elisa kit, by Beyotime (1 mentions)
5 protocols using tnf alpha elisa kit
1
Quantifying Inflammatory Markers in Plasma
2
Quantification of Misfolded Proteins
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The tissues were homogenized, and the proteins were extracted and diluted using Cell Extraction Buffer PTR for assay. The cell culture supernatant was centrifuged at 2000 g (Thermo Fisher) for 10 min to remove the debris and diluted using Sample Diluent NS, and then collected for assay. The cells were rinsed twice and incubated in Cell Extraction Buffer PTR on ice for 20 min and centrifuged at 18000 × g (Thermo Fisher) for 20 min, and then the supernatants were collected and diluted using Cell Extraction Buffer PTR for assay. The misfolded proteins in the medium or cells were quantified using tau ELISA Kit (Abcam, ab273617) or alpha-synuclein ELISA Kit (Abcam, ab260052). TNF-α, IL-1β, and IL-6 in the brain tissues were quantified using TNF alpha ELISA Kit (Abcam, ab208348), IL-1β ELISA Kit (Abcam, ab197742), or IL-6 ELISA Kit (Abcam, ab222503). The experimental procedures were performed according to the manufacturer's instructions.
3
Assessing Therapeutic Potential of Sarpogrelate
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Sarpogrelate was obtained from Shanghai Linebon Ltd., Shanghai, China. Bromocriptine was sourced from Novartis, Italy. Cabergoline was purchased from Pfizer, Italy. Domperidone was purchased from Jamjoom Pharmaceuticals Co., KSA. Alloxan was obtained from Sigma-Aldrich (St Louis, MO, USA). Urea, creatinine, alkaline phosphatase, aspartate aminotransferase activity, LDH-1 assay kits, and the TNF alpha ELISA kit were obtained from Abcam, USA. The troponin I test kit was obtained from Encode Medical Engineering Company, Jeddah, KSA. 2,3,5-Triphenyltetrazolium chloride was obtained from Gold Biotechnology, USA.
4
Quantification of Inflammatory Cytokines
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Mouse tumor necrosis factor (TNF) alpha ELISA Kit (Abcam, ab46105), Mouse interleukin-1 (IL-1) beta ELISA Kit (Boster; EK0394), and Mouse IL-6 ELISA Kit (Beyotime; Shanghai, China; PI326) were used to measure TNF-α, IL-1β, and IL-6 concentrations, respectively, in samples of serum according to the manufacturer's instructions. The assay plates were read using a SpectraMax M5 microplate reader (Molecular Devices; Sunnyvale, CA, USA).
5
Pharmacological Intervention for Diabetic Management
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Sarpogrelate was obtained from Shanghai Linebon Ltd. Shanghai, China. Bromocriptine was from Novartis, Italy. Cabergoline was purchased from P zer, Italy. Domperidone was purchased from Jamjoom pharmaceutical company, KSA. Alloxan was obtained from Sigma-Aldrich (St Louis, MO, USA). Urea; Creatinine; Alkaline Phosphatase; Aspartate Aminotransferase Activity; LDH-1 assay kits; and TNF alpha ELISA Kit were obtained from Abcam company. Troponin I test kit was obtained from Encode Medical Engineering Company, Jeddah, KSA. 2,3,5-Triphenyltetrazolium chloride was obtained from Gold Biotechnology, USA.
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