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Docu ph meter

Manufactured by Sartorius
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Sourced in United States, Germany

The Docu-pH meter is a laboratory instrument designed to measure the pH of solutions. It provides accurate and reliable pH measurements, making it a essential tool for various applications in the scientific and industrial sectors.

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Lab products found in correlation

Multiskan go, by Thermo Fisher Scientific (2 mentions) Aa 6200 model, by Shimadzu (1 mentions) Agilent 1220 infinity lc system, by Agilent Technologies (1 mentions) 2100q portable turbidimeter, by HACH (1 mentions) 1220 infinity lc system, by Agilent Technologies (1 mentions) Milli q ultrapure water system, by Merck Group (1 mentions) Prodigy c18 column, by Phenomenex (1 mentions)

10 protocols using docu ph meter

1

Measuring Ammonia Emissions from Animal Excreta

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The concentration of ammonia (NH3) was measured by collecting 300 g of excreta sample from the bottom tray of each replicate cage in a plastic zipper bag. The bags were placed in a plastic box with a lid having two holes. One hole was sealed with a membrane filter of pore size 1.0 µm (Advantec®, Toyo Roshi Kaisha Ltd., Tokyo, Japan), and another was used to measure the gas emission. The samples were allowed to ferment at room temperature, and gas emission was recorded using a Gastec gas-sampling pump (AP-20, Gastec Corp., Kitagawa, Japan) and a detector tube (3 LA, 3M for NH3) at 0, 6, 12, 24, and 48 h. The NH3 concentration was expressed as ppm/100 mL.
The pH of feces was measured using a digital pH meter (Docu-pH meter, Sartorius, USA) after diluting 1 g of a fecal sample with 9 mL of distilled water.
Dilawar M.A., Mun H.S., Rathnayake D., Yang E.J., Seo Y.S., Park H.S, & Yang C.J. (2021). Egg Quality Parameters, Production Performance and Immunity of Laying Hens Supplemented with Plant Extracts. Animals : an Open Access Journal from MDPI, 11(4), 975.
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2

Chemical Composition Analysis of Pomegranate By-Products

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The by-product of Punica granatum L. (PGB) (Goheunggun cultivar, Korea) was collected from a juice manufacturing company and included the peel, rind and seeds after juice extraction. The by-products were dried in a forced air drying oven (Doori TEC, Doori TEC, FA, Co., Ltd, Seoul, Korea) at 80°C for 3 days to reduce the moisture content to less than 10%, after which the dried by-products were ground into powder using a milling machine. The samples of PGB were subsequently analyzed in triplicate for crude protein (CP), ether extract (EE), moisture and ash as described by Association of Official Analytical Chemists (AOAC, 2000 ). Trace mineral contents were determined using an Atomic Absorption Flame Emission Spectrophotometer (Model AA-6200, Shimadzu, Japan). The pH of PGB was measured using a digital pH meter (Docu-pH + meter, Sartorius, USA). The concentration of total polyphenols, total flavonoids and hydrolysable tannin contents (quantified by colorimetric analysis) in PGB were analyzed by a commercial analytical company; the Foundation of Agricultural Technology Commercialization and Transfer (FACT, Suwon-si, Gyeonggido, Korea). The analytical results of chemical composition, trace mineral contents, pH, total polyphenols, total flavonoids and hydrolysable tannins in PGB are shown in Table 1.
Ahmed S.T, & Yang C.J. (2017). Effects of Dietary Punica granatum L. By-products on Performance, Immunity, Intestinal and Fecal Microbiology, and Odorous Gas Emissions from Excreta in Broilers. The Journal of Poultry Science, 54(2), 157-166.
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3

HPLC Analysis Using Agilent 1220 LC

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An Agilent 1220 Infinity LC system (G4294B configuration; Agilent Technologies, Santa Clara, CA, USA), which consisted of a dual solvent deliver system, an auto sampler, and a diode array detector (DAD), was used. An ultrasonic bath (S 100 H, Elmasonic, Singen, Germany) and a Docu pH-meter (Sartorius, Bohemia, NY, USA) were used.
Ibrahim F., El-Deen A.K., El Abass S.A, & Shimizu K. (2016). An ecofriendly green liquid chromatographic method for simultaneous determination of nicotinamide and clindamycin phosphate in pharmaceutical gel for acne treatment. Journal of Food and Drug Analysis, 25(3), 741-747.
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4

pH-Dependent NMR Analysis of GB1 Protein

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13C-15N GB1 was dissolved in 100 mM KCl buffer in 90% H2O/10% D2O containing 100 μM DSS (used as internal reference) to a protein concentration of 1 mM. The pH was measured using a Docu-pH meter (Sartorius) calibrated with standard solutions. The initial pH was 6.50. For each pH step, the pH was adjusted with microliter additions of 0.15 M NaOH solution. The concentration of added salt amounted to less than 1 mM. Spectra were collected in steps of ~0.4 pH units from pH 6.5 to 8.0. Data were processed using Bruker TopSpinTM 4.0 and analyzed with CCPNMR53 (link) for cross-peak assignment and height extraction. Further details are available in the Supplementary Information, Supplementary Methods section.
Faustino A.F., Barbosa G.M., Silva M., Castanho M.A., Da Poian A.T., Cabrita E.J., Santos N.C., Almeida F.C, & Martins I.C. (2019). Fast NMR method to probe solvent accessibility and disordered regions in proteins. Scientific Reports, 9, 1647.
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5

Calcium Hydroxide Disinfection at Outdoor and Indoor Locations

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Ca(OH)2 powder was used for stand-by disinfection at four outdoor locations (soil, grass, road, and in front of a barn, Supplementary Fig. 1). For stand-by disinfection, 0.5–1 kg/m2 Ca(OH)2 powder was spread, such that 1 kg of Ca(OH)2 powder for 1 m2 was spread at the four outdoor locations (Fig. 2a). To measure pH, 1 g of disinfectant was collected in a 15 mL plastic tube, 2.5 mL of distilled water was added, and the mixture was stirred. The suspension was left for 1 h, and the pH of the supernatant was measured by a pH meter (Docu-pH meter, Sartorius, New York, USA).
For the indoor experiment, a paper box was placed on artificial plastic grass (440–0011, Mizushima, Osaka, Japan), and 62.5 g of disinfectant was spread on the paper box (Fig. 2c). Water was sprayed daily (200 cm3/day) to match the annual precipitation in Hokkaido, Japan25 . This amount was determined as follows. Since average rainfall in Hokkaido from 1986 to 2015 was 114.8 cm/year (0.315 cm/day) and the size of the paper box was 25 × 25 cm, the daily watering rate was set to 25 × 25 × 0.32 cm = 200 cm3. Water (200 cm3) was applied once a day using a watering can. pH was measured in the same way as in the outdoor experiment.
Matsuzaki S., Azuma K., Lin X., Kuragano M., Uwai K., Yamanaka S, & Tokuraku K. (2021). Farm use of calcium hydroxide as an effective barrier against pathogens. Scientific Reports, 11, 7941.
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6

pH Measurement of Longissimus Dorsi Muscle

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The pH of the Longissimus dorsi muscle was measured using a digital pH meter (Docu-pH+ meter, Sartorius, Bohemia, NY, USA). The Longissimus dorsi muscle sample (4 g) was measured in a Falcon tube, after which deionized water (36 mL) was added, and then it was homogenized properly (30 s, 13,000 rpm, 25 °C) for mixing and filtered. Following homogenization and filtering, the pH value of the slurry was determined and recorded.
Bostami A.B., Mun H.S, & Yang C.J. (2023). Longissimus dorsi Muscle’s Chemical Composition, Fatty Acid Pattern, and Oxidative Stability in Korean Hanwoo Finishing Cattle Following Slaughtering and Stunning with or without Brain Disruption and State of Consciousness. Foods, 12(5), 928.
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7

Aqueous pH Determination via Sartorius Meter

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The samples were dissolved in distilled water, and the pH values were determined using a pH meter (DOCU-pH Meter, Sartorius, Göttingen, Germany).
Ichikawa N., Ng L.S., Makino S., Goh L.L., Lim Y.J., F., Sasaki H., Shibata S, & Lee C.L. (2022). Solid-State Fermented Okara with Aspergillus spp. Improves Lipid Metabolism and High-Fat Diet Induced Obesity. Metabolites, 12(3), 198.
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8

Measuring pH and Lipid Oxidation in Meat

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To determine the pH, approximately 5 g of Longissimus dorsi muscle (in triplicate) was cut into small pieces and homogenized with 45 mL distilled water for 60 s in an Ultra-Turrax (Janke and Kunkel, T25, Staufen Germany). Immediately after homogenization, the pH was measured using a digital pH meter (Docu-pH + meter, Sartorius, Columbus, OH, USA).
The thiobarbituric acid reactive substances (TBARS) were evaluated (in triplicate) using the procedure described by Witte et al. [31 (link)]. Briefly, 5 g of meat samples were mixed with 25 mL of 20% trichloroacetic acid (TCA) and homogenized for 30 s. Distilled water was added to prepare 50 mL of homogenate samples for centrifugation (3000× g, 4 °C, and 10 min). The supernatant was filtered through filter paper (Hyundai Co., Ltd., Seoul, Korea). Then, 5 mL of the filtrate was kept at room temperature for 15 h, and the absorbance was determined using a UV/VIS spectrophotometer (M2e, Molecular Devices, Sunnyvale, CA, USA). The TBARS value is expressed as micromoles of MDA/kg of meat.
Shin Y.G., Rathnayake D., Mun H.S., Dilawar M.A., Pov S, & Yang C.J. (2021). Sensory Attributes, Microbial Activity, Fatty Acid Composition and Meat Quality Traits of Hanwoo Cattle Fed a Diet Supplemented with Stevioside and Organic Selenium. Foods, 10(1), 129.
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9

Detailed Physicochemical Analysis of Samples

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The pH, titratable acidity (TA) and soluble solid (SS) analyses were conducted as described by William (2005) (link). The pH was measured using a calibrated pH meter (Sartorius Docu-pH meter, Germany). The TA analysis was performed using the potentiometric titration method, and the results were obtained as tartaric acid equivalent (g/L). A portable refractometer (Hanna HI 96801, USA) was used to measure SS (°Bx).
The absorbance values of the samples were measured using a spectrophotometer (Thermo scientific, Multiskango, Finland) at 420, 520 and 620 nm, and the color intensity (CI) values were calculated using the formula below (1). CI=A420+A520+A620
(1) The turbidity values of the samples were measured using a portable turbidimeter (Hach 2100Q Portable Turbidimeter, China), and the results were expressed in the nephelometric turbidity unit (NTU)
Güler A., & Candemir A. (2020). Determination of physicochemical characteristics, organic acid and sugar profiles of Turkish grape juices. International Journal of Agriculture Environment and Food Sciences, 4(2), 149-156.
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10

HPLC Separation of Compounds with Formic Acid

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Chromatographic separation was completed using Agilent 1220 Infinity LC system, equipped with EZ-chrome software and a photodiode array detector. Separation was accomplished with a Phenomenex Prodigy C18 column (4.6 × 250 mm, 5 μm) at 40°C with a mobile phase comprising of water (solvent A) and methanol (solvent B) both contain 0.1% formic acid and pumped at a flow rate of 1.0 mL/min in a gradient manner. The gradient program started with A:B (40:60, v/v) held for 1 min, changed to A:B (10:90, v/v) over 9 min, and held for 5 min. Then it was returned to initial conditions A:B (40:60, v/v) over 1 min and held for 9 min for a total run time of 25 min. Detection wavelength is 270 nm and an injection volume of 10 μL. A Docu pH-meter (Sartorius, Bohemia, NY) was used for adjusting the pH. Milli-Q water was obtained by a Milli-Q Ultrapure water system (Millipore Corp., Bedford, MA).
El-Deen A.K, & Shimizu K. (2019). Application of D-Limonene as a Bio-based Solvent in Low Density-Dispersive Liquid-Liquid Microextraction of Acidic Drugs from Aqueous Samples. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 35(12).
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