Log In Sign up
The largest database of trusted experimental protocols

Hcc827

Manufactured by Corning
Visit Supplier
Sourced in United States, China

HCC827 is a lab equipment product manufactured by Corning. It is designed for general laboratory use, with a focus on providing reliable and consistent performance. The core function of HCC827 is to facilitate common laboratory procedures and tasks. Further details on the specific intended use or features of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

H1975, by Corning (6 mentions) H1975, by American Type Culture Collection (4 mentions) Hcc827, by American Type Culture Collection (4 mentions) Rpmi 1640 medium, by Corning (3 mentions) Rpmi 1640 media, by Corning (3 mentions) H3255, by Lonza (3 mentions) Begm media, by Lonza (3 mentions) H1650, by Corning (2 mentions) F 12k nutrient mixture, by Thermo Fisher Scientific (2 mentions) H2122, by American Type Culture Collection (2 mentions) Dmem media, by Corning (2 mentions) Rpmi 1640, by Corning (2 mentions) Hek293t, by American Type Culture Collection (2 mentions) Bronchial epithelial cell growth medium, by Lonza (1 mentions) Beas 2b cell line, by National Collection of Authenticated Cell Cultures (1 mentions) H1355, by Thermo Fisher Scientific (1 mentions) Pc 9 cell line, by Merck Group (1 mentions) H1355, by American Type Culture Collection (1 mentions) H3255, by American Type Culture Collection (1 mentions) Afatinib, by Selleck Chemicals (1 mentions)

8 protocols using hcc827

1

Cultured Cell Line Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China) and cultured in Bronchial Epithelial Cell Growth Medium (Lonza, CC-3170). The lung cancer cell lines, including HCC827, H1650, A549, and H1975, were purchased from the CASCB (China) with STR documents and were cultured in RPMI-1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.
Li X., Chen X., Yuan Y., Zhai R., Cho W.C, & Jiang X. (2022). Identifying Immune Cell Infiltration and Effective Diagnostic Biomarkers in Lung Adenocarcinoma by Comprehensive Bioinformatics Analysis and In Vitro Study. Frontiers in Oncology, 12, 916947.
+ Open protocol
+ Expand
2

Authenticated Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human cancer cell lines H1975, HCC827, H2122, CHO, CHL and H1355 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). PC9 cell line was purchased from the Sigma-Aldrich (St. Louis, MO, USA). A549, H3255 were purchased from Cobioer Biosciences CO., LTD (Nanjing, China). H1975, PC-9, HCC827 and EGFR mutant isogenic BaF3 cells were cultured in RPMI 1640 media (Corning, USA) with 10% fetal bovine serum (FBS) and supplemented with 2% L-glutamine and 1% penicillin/streptomycin. A549 and H1355 were cultured in F-12K Nutrient Mixture (kaighn's Modification) (Gibco, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. H3255 was cultured in BEGM media (LONZA, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep.
We have authenticated the following cell lines through cell line short tandem repeat (STR) profiling (GENEWIZ, Suzhou, China): H1975, PC9, H3255, HCC827, A549, H2122, H1703. All cell lines matched >90% with lines listed in the ATCC, DSMZ or JCRB Cell Line Bank STR Profile Information.
Hu C., Wang A., Wu H., Qi Z., Li X., Yan X.E., Chen C., Yu K., Zou F., Wang W., Wang W., Wu J., Liu J., Wang B., Wang L., Ren T., Zhang S., Yun C.H., Liu J, & Liu Q. (2017). Discovery and characterization of a novel irreversible EGFR mutants selective and potent kinase inhibitor CHMFL-EGFR-26 with a distinct binding mode. Oncotarget, 8(11), 18359-18372.
+ Open protocol
+ Expand
3

Culturing Human Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human cancer cell lines A549, A431, H3255, H1975, PC-9, HCC827, H23, H460, A549, H358 and H2122 cells were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). H1975, PC-9, HCC827, H23, H460, H358, H2122 and EGFR mutant isogenic BaF3 cells lines were cultured in RPMI 1640 media (Corning, USA) with 10% fetal bovine serum (FBS) and supplemented with 2% L-glutamine and 1% penicillin/streptomycin. A549 and A431 were cultured in DMEM media (Corning, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. H3255 was cultured in BEGM media (LONZA, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep.
Wu H., Wang A., Zhang W., Wang B., Chen C., Wang W., Hu C., Ye Z., Zhao Z., Wang L., Li X., Yu K., Liu J., Wu J., Yan X.E., Zhao P., Wang J., Wang C., Weisberg E.L., Gray N.S., Yun C.H., Liu J., Chen L, & Liu Q. (2015). Ibrutinib selectively and irreversibly targets EGFR (L858R, Del19) mutant but is moderately resistant to EGFR (T790M) mutant NSCLC Cells. Oncotarget, 6(31), 31313-31322.
+ Open protocol
+ Expand
4

Cell Line Authentication and Maintenance

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were obtained from ATCC and fingerprinted as in Barretina et al (Barretina et al., 2012 (link)). Cells were maintained in RPMI-1640 (NCI-H1299, HCC364, NCI-H1975, and HCC827; Corning, Corning, NY), McCoy’s 5A (CALU1; Gibco, Waltham, MA) or DMEM (MGH-065; Invitrogen, Carlsbad, CA) supplemented with 2 mM glutamine, 50 U/mL penicillin, 50 U/mL of streptomycin (Gibco), and 10% fetal bovine serum (Sigma, St. Louis, MO), and incubated at 37°C in 5% CO2. MGH-065 cells were derived as previously described (Crystal et al., 2014 (link)). Cell lines were tested for mycoplasma prior to screening. Trametinib, vemurafenib, erlotinib, and afatinib, were purchased from Selleck Chemicals (Houston, TX). LDK-378 and Crizotinib were synthesized by the Novartis Global Discovery Chemistry Department.
Krall E.B., Wang B., Munoz D.M., Ilic N., Raghavan S., Niederst M.J., Yu K., Ruddy D.A., Aguirre A.J., Kim J.W., Redig A.J., Gainor J.F., Williams J.A., Asara J.M., Doench J.G., Janne P.A., Shaw A.T., McDonald III R.E., Engelman J.A., Stegmeier F., Schlabach M.R, & Hahn W.C. (2017). KEAP1 loss modulates sensitivity to kinase targeted therapy in lung cancer. eLife, 6, e18970.
+ Open protocol
+ Expand
5

NSCLC Cell Line Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HCC827, H1299, H1975, A549 and HEK293T NSCLC cell lines were obtained from the American Type Culture Collection (ATCC, USA) and the cell bank of China Academy of Sciences (Shanghai, China). The H1299, H1975, HCC827, A549 and HEK293T cells were cultured in DMEM or RPMI-1640 medium (Corning, USA) with 1% penicillin-streptomycin solution (HyClone, USA) and 10% fetal bovine serum which is made in America (FBS, Gibco, USA). Cells were cultured in 5% carbon dioxide cell incubator at 37˚C.
Qi P., Li Y., Liu X., Jafari F.A., Zhang X., Sun Q, & Ma Z. (2019). Cryptotanshinone Suppresses Non-Small Cell Lung Cancer via microRNA-146a-5p/EGFR Axis. International Journal of Biological Sciences, 15(5), 1072-1079.
+ Open protocol
+ Expand
6

Cell Culture Protocols for Lung Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human bronchial epithelial (BEAS2B) cell line and LUAD cell lines were purchased from the cell bank of Kunming Institute of Zoology and cultured in bronchial epithelial cell growth media (BEGM) (Lonza, Shanghai, CC-3170). HEK-293T was obtained from the American Type Culture Collection (ATCC). Lung cancer cell lines, including H1650, HCC827, and H1975 were purchased from Cobioer (Shanghai, China) with STR document; H1650, HCC827, and H1975 cells were all cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Corning, Shanghai) supplemented with 10% fetal bovine serum (Cat. No. 10099141C, Gibco, New York, USA) and 1% penicillin/streptomycin.
Li X., Yuan Y., Pal M, & Jiang X. (2022). Identification and Validation of lncRNA-SNHG17 in Lung Adenocarcinoma: A Novel Prognostic and Diagnostic Indicator. Frontiers in Oncology, 12, 929655.
+ Open protocol
+ Expand
7

Lentiviral Transduction of GFP1-10 in Cell Lines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HCC827, MBA-MD-231, and MCF7 cell lines were purchased from the Research Facilities of Peking Union Medical College (PUMC) Cell Bank (Beijing, China). The A549 cell line was purchased from Shanghai Biowing Biotechnology Co (Shanghai, China). A549 and MCF7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning, NY, USA) supplied with 10% fetal bovine serum (FBS) (Gemini Bio, CA, USA). HCC827 and MBA-MD-231 cells were maintained in RPMI 1640 medium (Corning, NY, USA) supplied with 10% FBS. 293F cells were grown in Freestyle™ 293 expression medium (OPM, Shanghai, China). All cells were maintained at 37 °C in a humidified chamber containing 5% CO2.
Lentiviral particles were produced to transfect GFP1-10 (#80409, Addgene, Watertown, MA, USA) gene into HCC827 cells according to the protocol of Lentivirus Production (Addgene) [46 (link)]. GFP1-10-positive cells were selected with puromycin (3 μg/mL).
Dou X., Xu Q., Dong B., Xu G., Qian N., Yang C., Li H., Chen L., Gao X, & Song H. (2022). Anti-c-MET Fab-Grb2-Gab1 Fusion Protein-Mediated Interference of c-MET Signaling Pathway Induces Methuosis in Tumor Cells. International Journal of Molecular Sciences, 23(19), 12018.
+ Open protocol
+ Expand
8

Culturing Human Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human cancer cell lines H1975, HCC827, and A549 cells were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). A431 was purchased from Cobioer Biosciences CO., LTD (Nanjing, China). H1975, HCC827 and EGFR mutant isogenic BaF3 cells lines were cultured in RPMI 1640 media (Corning, USA) with 10% fetal bovine serum (FBS) and supplemented with 2% L-glutamine and 1% penicillin/streptomycin. A549 was cultured in F-12K Nutrient Mixture (kaighn's Modification) (Gibco, USA), and A431 was cultured in DMEM media (Corning, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. H3255 was cultured in BEGM media (LONZA, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. All cell lines were maintained in culture media at 37°C with 5% CO2.
Wang A., Yan X.E., Wu H., Wang W., Hu C., Chen C., Zhao Z., Zhao P., Li X., Wang L., Wang B., Ye Z., Wang J., Wang C., Zhang W., Gray N.S., Weisberg E.L., Chen L., Liu J., Yun C.H, & Liu Q. (2016). Ibrutinib targets mutant-EGFR kinase with a distinct binding conformation. Oncotarget, 7(43), 69760-69769.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!