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Mouse ngal elisa kit

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The Mouse NGAL ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse Neutrophil Gelatinase-Associated Lipocalin (NGAL) levels in cell culture supernatants, urine, and serum. The kit utilizes a monoclonal antibody specific for mouse NGAL that has been pre-coated onto a microplate. NGAL present in the sample is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for mouse NGAL is added to the wells to detect the bound NGAL.

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Mouse cystatin c elisa kit, by BioVendor (1 mentions) Albuwell m, by Exocell (1 mentions)

Close spelling variants of this product

NGAL ELISA kit Mouse ELISA kits ELISAs

4 protocols using mouse ngal elisa kit

1

Serum NGAL and Cystatin C Quantification

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Blood samples were collected and centrifuged at 10,000 rpm for 10 minutes to obtain serum. The concentration of serum neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C were measured using the Mouse NGAL ELISA Kit (R&D Systems) and Mouse Cystatin C ELISA Kit (Bio Vendor R&D) according to the manufacturer's instruction.
Kyo S., Murata K., Kawatou M., Minatoya K., Sunagawa G.A, & Masumoto H. (2022). Quiescence-inducing neurons-induced hypometabolism ameliorates acute kidney injury in a mouse model mimicking cardiovascular surgery requiring circulatory arrest. JTCVS Open, 12, 201-210.
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2

Serum Biomarkers in Mouse Model

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Serum was obtained from blood drawn from mouse hearts, and the levels of blood urea nitrogen (BUN) and creatinine were measured. The serum levels of LCN2 and G-CSF were determined by enzyme-linked immunosorbent assay (ELISA) using a Mouse NGAL ELISA kit (R&D Systems, Minneapolis, MN, USA) and a Mouse G-CSF Quantikine ELISA kit (BioPorto Diagnostics A/S, Hellerup, Denmark).
Ishii T., Okuyama T., Noguchi N., Nishidono Y., Okumura T., Kaibori M., Tanaka K., Terabayashi S., Ikeya Y, & Nishizawa M. (2019). Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice. Journal of Natural Medicines, 74(1), 51-64.
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3

Serum NGAL Levels in Mouse Sepsis

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Six-week old female outbred Swiss Webster mice (20–25 g) were used in this experiment. The room was set on a twelve hour light cycle and the temperature is set to 70 °F. The humidity was set to 30%. After 3 days of acclimation, mice were randomly divided into 3 groups (n=6 in each group): vehicle group, colistin sulfate group and biphenyl-macolacin group. Mice were subcutaneous injected (SC) with 100 μL of 0.9 % saline (vehicle group), 20 mg/kg colistin sulfate or 20 mg/kg biphenyl-macolacin for 7 consecutive days. Serum was collected from blood samples 12 h after the last dose. The concentration of serum Neutrophil Gelatinase Associated Lipocalin (NGAL) was measured using a commercially available mouse NGAL ELISA Kit (R&D system, USA). The ELISA assay followed the manufacture’s instruction. The animal study was ethically reviewed and carried out in accordance with the Institutional Animal Care and Use Committees at the Rockefeller University under protocol 19032-H.
Vásquez-Vivar J., Kalyanaraman B., Martásek P., Hogg N., Masters B.S., Karoui H., Tordo P, & Pritchard KA J.r. (1998). Superoxide generation by endothelial nitric oxide synthase: The influence of cofactors. Proceedings of the National Academy of Sciences of the United States of America, 95(16), 9220-9225.
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4

Creatinine, NGAL, and Fructose Assay

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Serum and urine creatinine were measured with Albuwell M (Exocell, Philadelphia, PA) and Creatinine LiquiColor Test (Enzymatic Methodology; Stanbio, Boerne, TX), respectively. Urine levels of NGAL were measured with a Mouse NGAL ELISA kit (R&D Systems, Minneapolis, MN). BUN was determined with an VetACe autoanalyzer. Urinary fructose was determined biochemically (EFRU-250; Bioassay Systems) following the manufacturer's instructions. This kit employs fructose dehydrogenase to initiate the reaction, a plant enzyme not found in mammals thus not interfering with activities of endogenously present mammalian fructokinase.
Andres-Hernando A., Li N., Cicerchi C., Inaba S., Chen W., Roncal-Jimenez C., Le M.T., Wempe M.F., Milagres T., Ishimoto T., Fini M., Nakagawa T., Johnson R.J, & Lanaspa M.A. (2017). Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice. Nature Communications, 8, 14181.
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