Mice were anesthetized with isoflurane, and echocardiography was performed using a Vevo 2100 Micro‐Imaging System (VisualSonics Inc.) with an 18‐MHz to 38‐MHz linear array ultrasound transducer. The acquisition and analysis were done by a single experienced researcher in a blinded manner. Systolic and diastolic dimensions were determined by M‐mode in the parasternal short axis view at the level of the papillary muscles. Ejection fraction, fractional shortening, left ventricle (LV) end systolic and diastolic diameters, LV end systolic and diastolic volumes, and systole and diastole LV posterior wall thicknesses were calculated based on the M‐mode parasternal short axis view. Systolic and diastolic LV area and ascending aorta diameter were determined by the 2‐dimensional long axis view. Aorta velocity peak gradient was measured by conventional pulsed‐wave spectral Doppler analysis of mitral valve inflow patterns.
Vevo 2100 micro imaging system
Manufactured by Fujifilm
Sourced in Canada
The Vevo 2100 Micro-Imaging system is a high-resolution, non-invasive imaging device designed for preclinical research applications. It uses high-frequency ultrasound technology to capture detailed images of small animals and other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using vevo 2100 micro imaging system
1
Echocardiographic Assessment of Cardiac Function
2
Echocardiographic Analysis of Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treatment, mice underwent an echocardiographic by using a Vevo 2100 Micro-imaging System (Visual Sonics, Toronto, ON, Canada) with a 30-MHz probe. The mice were anesthetized using 2.5% isoflurane. Each mouse was transferred to dorsal recumbency and then was placed on a heated imaging platform after fully anesthetized. The following parameters were calculated: LVEF, LVFS, IVS; d, LVPW; d, LVID; d, LV Vol; d, LV Mass, and RWT. All measurements were averaged for three consecutive cardiac cycles by an experienced technician who was the study groupings.
3
Echocardiographic Assessment of BXD Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transthoracic two-dimensional and Doppler echocardiography was used to evaluate heart function and morphology in BXD mice. Experiments were conducted in BXD, B6, and D2 strains (44 strains) at 4–6 months of age (n > 5/strain). The chest of the mice was treated with a chemical hair remover day prior. Mice were anesthetized by oxygenated 1–2% isoflurane, and core temperature and heart rate were maintained using a heated platform set at 37°C, while echocardiography was performed using a Vevo2100 Micro-Imaging System (VisualSonics Inc., Toronto, Canada). Fractional shortening (FS%), ejection fraction (EF%), left ventricular volumes end-diastolic and end-systolic (LVVol;d and LVVol;s, respectively), LV internal diameters end-diastolic and end-systolic (LVID;d and LVID;s, respectively), interventricular septum end-diastolic and end-systolic (IVS;d and IVS;s, respectively) and LV posterior wall thickness end-diastolic and end-systolic (LVPW;d and LVPW;s, respectively), stroke volume and cardiac output (CO) were calculated as reported previously (19 (link)).
4
Echocardiography Evaluation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two-dimensional and M-mode transthoracic echocardiography were performed under isoflurane anesthesia as previously described using a Vevo 2100 Micro-Imaging system (VisualSonics, Inc.) and a 40 MHz transducer [1 (link)]. Off-line analyses were performed by investigators blinded to genotype.
5
Echocardiographic Evaluation of Cardiac Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two-dimensional and M-mode transthoracic echocardiography were performed under isoflurane anesthesia (1.75% isoflurane) as previously described using a Vevo 2100 Micro-Imaging system (VisualSonics, Inc.) and a 40 MHz transducer (Acehan et al., 2011 (link); Huang et al., 2017 (link)). Off-line analyses were performed by investigators blinded to treatment and genotype.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!