Cells growth was determined via XTT assays (Roche). Cell migration assays were performed using modified Boyden chambers (Chemicon) as previously described 42 (link). For invasion assays through Matrigel, 2 × 104 cells were placed into the top well of Boyden chambers containing growth factor-reduced Matrigel extracellular basement membrane over a polyethylene terephthalate membrane (8-mm pores; BD Biosciences). Following 24 h culture, Matrigel was removed and invaded cells were fixed and stained. Cells adhering to the bottom of the membrane were counted.
Matrigel extracellular basement membrane
Manufactured by BD
Matrigel extracellular basement membrane is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is a complex mixture of extracellular matrix proteins, growth factors, and other components.
Automatically generated - may contain errors
2 protocols using matrigel extracellular basement membrane
1
Cell Migration and Invasion Assays
2
Cell Migration and Invasion Assays
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Cells growth was determined via XTT assays (Roche). Cell migration assays were performed using modified Boyden chambers (Chemicon) as previously described 42 (link). For invasion assays through Matrigel, 2 × 104 cells were placed into the top well of Boyden chambers containing growth factor-reduced Matrigel extracellular basement membrane over a polyethylene terephthalate membrane (8-mm pores; BD Biosciences). Following 24 h culture, Matrigel was removed and invaded cells were fixed and stained. Cells adhering to the bottom of the membrane were counted.
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