Mass spectrometry grade methanol

MgT was purchased from Shanghai Yuanye Biotechnology Co., CAS number is 778571–57-6, molecular formula is C8H14MgO10, purity is more than 95%. Trypsin Golden (Promega, United States), dithiothreitol (DTT, Sigma, Germany), iodoacetamide (IAA, Sigma, Germany), ammonium bicarbonate NH4HCO3 (Sigma, Germany), urea (Sigma, Germany), pure water (China Wahaha Company), mass spectrometry grade methanol (Thermo Fisher Scientific, United States), mass spectrometry grade acetonitrile (Thermo Fisher Scientific, United States), mass spectrometry grade purified water (Thermo Fisher Scientific, United States), Tris-base (Promega, United States), thiosulfate (Sigma, Germany), thiamine (Sigma, Germany), thiophene (Promega, United States), and thiourea (Sigma, Germany) were obtained.

Mass spectrometry-grade methanol, acetonitrile, and formic acid were acquired from Thermo Fisher Scientific Inc. (Loughborough, United Kingdom). RS was purchased from Jinan Jianlian TCM Pharmacy (Batch No. 210415, Shandong, China) and authenticated and conformed to the standards of the Pharmacopoeia of the People's Republic of China. To prepare the RS extract, 3 kg of RS was reconstituted with 60 liters of water and extracted twice. The extraction yield was 13.4% (g/g). Details of the three major compounds in the RS extract are shown in Table S1 and Figure S1 in the Supplementary Material. The extract was dried in an oven and dissolved in distilled water to a final concentration of 1 g/ml. Valsartan (Lot X2882, Beijing Novartis Pharma Ltd., China) was also dissolved in distilled water to a final concentration of 1.44 mg/ml. N′-nitro-L-arginine (L-NNA) (Lot BCCD9665) was obtained from Sigma Aldrich (St. Louis, MO, United States), and N^G-Nitro-L-arginine Methyl Este (L-NAME) (Lot N1101A) was purchased from Meilunbio (Dalian, Liaoning, China).

A. senticosus leaves were collected at Muling County (Heilongjiang Province, China). Mass spectrometry-grade methanol and acetonitrile were obtained from Thermo Fisher (Geel, Belgium). HPLC-grade formic acid was obtained from Dikma (Lake Forest, USA). Purified water was obtained from Watsons (China). Other reagents were purchased from local sources and of analytical grade.