Cpt 1b

Manufactured by Santa Cruz Biotechnology

CPT-1b is a laboratory reagent used in biochemical research. It functions as an enzyme involved in the transportation of fatty acids into the mitochondria for energy production. The core function of CPT-1b is to facilitate this metabolic process.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using cpt 1b

Muscle Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from 40 mg frozen gastrocnemius muscle samples as previously described [41 (link)]. Protein concentration was measured with a Pierce BCA Protein Assay kit (23225; Thermo Scientific). Antibodies and their sources are: Adiponectin (BS6961, Bioworld), AdipoR1 (BS6797, Bioworld), AdipoR2 (14361-1-AP, Proteintech), AMPKα112 (BS1009, Bioworld), p-AMPKα1/2 (sc-33524, Santa Cruz), PGC-1α (sc-13067, Santa Cruz), UCP3 (BS2849, Bioworld), UCP2 (BS2917, Bioworld), GPR43 (sc-32906, Santa Cruz), GPR41 (sc-98332, Santa Cruz), CPT-1b (sc-20670, Santa Cruz), COX4 (MB0102, Bioworld), HDAC1 (BS6485, Bioworld), GAPDH (AP0063, Bioworld), β-actin (AP0063, Bioworld). The phosphorylated AMPK was normalized by the total AMPK.

Hong J., Jia Y., Pan S., Jia L., Li H., Han Z., Cai D, & Zhao R. (2016). Butyrate alleviates high fat diet-induced obesity through activation of adiponectin-mediated pathway and stimulation of mitochondrial function in the skeletal muscle of mice. Oncotarget, 7(35), 56071-56082.

+ Open protocol

+ Expand

Kidney Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney tissue was minced in lysate buffer (150 mM NaCl, 50 mM Tris-HCL, 1 mM EDTA, and 2% SDS plus phosphatase and protease inhibitors), sonicated, centrifuged, reduced with dithiothreitol, and quantified using the BCA protein assay (Thermo Fisher Scientific). Proteins were separated by SDS-PAGE, transferred to nitrocellulose membranes, and incubated with the following primary antibodies: CPT1A (Abcam, ab128568), CPT1B (Santa Cruz, sc-20670), VDAC (Abcam, ab154856), p16 (Abcam, ab211542), GAPDH (Santa Cruz, sc-25778), and β-actin (Cell Signaling, 3700). Appropriate HRP-conjugated secondaries were used followed by ECL, and bands were detected by GeneGnome (Syngene) and quantified by ImageJ.

Hammoud S., Ivanova A., Osaki Y., Funk S., Yang H., Viquez O., Delgado R., Lu D., Phillips Mignemi M., Tonello J., Colon S., Lantier L., Wasserman D.H., Humphreys B.D., Koenitzer J., Kern J., de Caestecker M., Finkel T., Fogo A., Messias N., Lodhi I.J, & Gewin L.S. (2024). Tubular CPT1A deletion minimally affects aging and chronic kidney injury. JCI Insight, 9(6), e171961.

+ Open protocol

+ Expand

Immunoblotting for Metabolic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was conducted as previously described (21) using primary antibodies of UCP-1 (#ab23841; Abcam, Cambridge, MA), carnitine palmitoyltransferase 1b (#sc20670; CPT1b, Santa Cruz Biotechnology Inc., Santa Cruz, CA), and β-actin (#sc1616; Santa Cruz) at 1:400, 1:200, and 1:1000 dilution, respectively. Horseradish peroxidase-conjugated secondary antibodies were probed at 1:1000 dilutions at room temperature for 2 h. Blots were exposed to chemiluminescence reagent and X-ray films were developed using a SRX-101A Konica Minolta film developer. Two samples from HL, HL+CLA, HLS, and HLS+CLA treatments were randomly selected and run on the same gel, and are representative of other samples within each treatment group. Densitometry was conducted using a Kodak 4400 CF Image Station as described previously [21 (link)].

Shen W., Baldwin J., Collins B., Hixson L., Lee K.T., Herberg T., Starnes J., Cooney P., Chuang C.C., Hopkins R., Reid T., Gupta S, & McIntosh M. (2015). Low level of trans-10, cis-12 conjugated linoleic acid decreases adiposity and increases browning independent of inflammatory signaling in overweight Sv129 mice. The Journal of nutritional biochemistry, 26(6), 616-625.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!