Rg9 1

Manufactured by BioXCell

The RG9–1 is a laboratory equipment designed for specialized research purposes. It serves as a core functional component in various scientific applications. A detailed description of its intended use or capabilities cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Recombinant mouse galectin 9, by R&D Systems (1 mentions) Lactose, by Merck Group (1 mentions) Rmt3 23, by BioXCell (1 mentions) Anti il 10r 1b1.3a, by BioXCell (1 mentions) Anti hmgb 1 pab, by Thermo Fisher Scientific (1 mentions) Anti hmgb 1 pab, by Shino-Test (1 mentions)

2 protocols using rg9 1

Quantification of TIM-3 and HMGB1 Interactions

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Mouse anti-human IgG (Fc) coated polystyrene beads (Spherotech) were coupled to 40 μg/ml recombinant mouse TIM-3-Fc (R&D Systems) by incubation at room temperature for 1 hr. Galectin-9 binding was measured by incubating 1 μg/ml recombinant mouse galectin 9 (R&D Systems) in the presence or absence of 10 μg/ml galectin-9 blocking antibody (RG9–1, BioXCell) or 100 mM lactose (Sigma-Aldrich) for 30 minutes. Galectin-9 bound to beads was then detected by incubation with 0.5 μg/ml of an APC conjugated anti-mouse galectin-9 antibody that recognizes the linker region (clone 108A2), with detection by flow cytometry. Binding of FLAG-tagged HMGB1ΔC was determined using an anti-FLAG-PE antibody. Binding of B-DNA/HMGB1ΔC complexes was detected by measuring rhodamine fluorescence by flow cytometry. Rat IgG_2a isotype control or anti-HMGB1 (clone 3E8) were pre-incubated with HMGB1 at 10 μg/ml, prior to their addition to TIM-3-Fc-coated polystyrene beads.

de Mingo Pulido Á., Hänggi K., Celias D.P., Gardner A., Li J., Batista-Bittencourt B., Mohamed E., Trillo-Tinoco J., Osunmakinde O., Peña R., Onimus A., Kaisho T., Kaufmann J., McEachern K., Soliman H., Luca V.C., Rodriguez P.C., Yu X, & Ruffell B. (2021). The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake. Immunity, 54(6), 1154-1167.e7.

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Blockade of Immune Checkpoint Proteins

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In vivo whole-animal blockade of HMGB-1, PD-L1, and Tim-3 was conducted by intraperitoneal (IP) injection of 300 μg of anti-HMGB-1 (pAb; Shino-Test Corporation, Kanagawa, Japan), anti-PD-L1 (10F.9G2), or anti-Tim-3 (RMT3-23; BioXCell, West Lebanon, NH). For in vitro and in vivo lymph node blockade, CD8⁺ T_reg cells were precoated with 20 μg/mL of anti-PD-L1 and/or anti-Tim-3 for 1 hour at 37°C. Recombinant mouse Gal-9 (rGal-9; 1.0 μg/mL; R&D Systems), 20 μg/mL of anti-Gal-9 (RG9-1), 20 μg/mL of anti-IL-10R (1B1.3A; BioXCell), and 0.5 μg/mL of anti-HMGB-1 (pAb; eBioscience) were added to culture media in relevant experiments.

Dolina J.S., Braciale T.J, & Hahn Y.S. (2014). Liver-primed CD8+ T cells suppress antiviral adaptive immunity through galectin-9-independent T-Cell immunoglobulin and mucin 3 engagement of high-mobility group box 1 in mice. Hepatology (Baltimore, Md.), 59(4), 1351-1365.

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