Sc79 s7863

Manufactured by Selleck Chemicals

Sourced in China, United States

SC79 (S7863) is a laboratory reagent produced by Selleck Chemicals. It is a small molecule that functions as a selective inhibitor. The core function of this product is to inhibit a specific target, without further interpretation of its intended use.

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Lab products found in correlation

6 protocols using sc79 s7863

Investigating Multidrug Resistance Mechanisms

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MSA (541,281) and dansylcadaverine (30,432) were purchased from Sigma-Aldrich (St. Louis, United States). Cisplatin was purchased from Qilu Pharmaceutical (Jinan, China). CQ (MB1668) and 3-MA (MB5063) were purchased from meilunbio (Dalian, China). SC79 (s7863) and MHY1485 (s7811) were purchased from Selleck (shanghai, China). The following antibodies were used, MDR1 (22336-1-AP), ALDH1A1 (60171-1-Ig), Bcl-2 (12789-1-AP), Bax (50,599-2-lg), Caspase-3 (19677-1-AP), Parkin (14060-1-AP), P62 (66,184-1-lg), LC3 (14600-1-AP), Akt (10176-2-AP), phospho-Akt (ser473) (66,444-1-lg), phospho-Akt (thr308) (29163-1-AP), mTOR (66,888-1-lg) were purchased from Proteintech (Wuhan, China). phospho-mTOR (ser2448) (#5536), GAPDH (#5174) were purchased from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies (ab205718, ab205719) were procured from Abcam (Cambridge, United Kingdom).

Xin M., Gao Q., Xiang X., Xu J., Jiao Y., Li X., Zhang X, & Jia X. (2022). Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells. Frontiers in Pharmacology, 13, 890974.

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Cerebral I/R Injury Protection via PI3K/Akt

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The rats were divided into sham, vehicle, MCAO, SC79, and SC79+LY294002 groups. 60 min prior to cerebral ischemia (60 min) followed by reperfusion, SC79 (S7863) (Selleck, Shanghai, China) was applied via intraperitoneal injection at a concentration of 0.04 mg/g of body weight. The mice were subjected to focal cerebral ischemia (60 min) followed by reperfusion (24 hours).
To determine the role of PI3K/Akt signaling in SC79-induced protection against cerebral I/R injury, the PI3K inhibitor LY294002 (Sigma, Shanghai, China) (1 mg/25 g body weight) was given to mice 15 min before SC79 administration according to the previous report [15 (link)]. Rats in the model and sham groups were injected with an equal volume of saline.

Luan Q., Pan L., He D., Gong X, & Zhou H. (2018). SC79, the AKT Activator Protects Cerebral Ischemia in a Rat Model of Ischemia/Reperfusion Injury. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 5391-5397.

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Investigating Pyroptosis Pathway Activation

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Primary antibodies against DNA-PK (19983-1-AP), mTOR (28273-1-AP), p-mTOR (67778-1-Ig), S6K1 (14485-1-AP), Akt (10176-2-AP), p-Akt (66444-1-Ig), Bax (50599-2-Ig), cytochrome c (10993-1-AP), and GAPDH (60004-1-Ig), as well as secondary antibodies, were obtained from Proteintech Group, Inc. (Wuhan, China). Primary antibodies against caspase-1 (ab179515), GSDMD (ab219800), GSDMD-N (ab215203), caspase-3 (ab32351), GSDME and GSDME-N (ab215191), and p-DNA-PK (ab124918) were purchased from Abcam (Cambridge, MA, USA). Anti-γH2AX (9718) and anti-p-S6K1 (9205S) were purchased from Cell Signaling Technology (Danvers, MA, USA). The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Zhang T., Liu M.Q., Xie G.S., Wu D.M., Luo P.W., Liu T., Deng S.H., Wang Y.Y., He S., Zhou Y., Zhou J, & Xu Y. (2023). CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway. Journal of Cancer, 14(8), 1350-1361.

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Phosphorylation Pathway Modulation Protocol

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GNE-477 [S6516, purity (98.43%)] (Figure 1A) and the AKT phosphorylation activator SC79 (S7863, purity (>97%)) were both purchased from Selleckchem (Houston, TX, United States), and GNE-477 was dissolved and preserved in dimethyl sulfoxide (DMSO) obtained from Servicebio (G5051, Wuhan, China). The following antibodies were used: anti-AKT (product no.4691, Cell Signaling Technology (CST), United States), anti-phospho-AKT (product no. 4060; CST, United States), anti-CyclinD1 (60,186-1- Ig, Proteintech, Wuhan, China), anti-MMP2 (10,373-2- AP, Proteintech, Wuhan, China), anti-Bax (50599-2-Ig, Proteintech, Wuhan, China), anti-Bad (product no. 9239,CST,United States), anti-Bcl-2 (127891-AP,Proteintech, Wuhan, China), anti-mTOR (20657-1-AP, Proteintech, Wuhan, China), anti-phospho-mTOR (ab109268, Abcam, United Kingdom), and anti GAPDH (60004-1-Ig, Proteintech).

Wang Y., Shen H., Sun Q., Zhao L., Liu H., Ye L., Xu Y., Cai J., Li Y., Gao L., Tan Y., Liu B, & Chen Q. (2021). The New PI3K/mTOR Inhibitor GNE-477 Inhibits the Malignant Behavior of Human Glioblastoma Cells. Frontiers in Pharmacology, 12, 659511.

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Huh7 and MHCC-97H Cell Culture and Drug Treatment

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Huh7 and MHCC-97H cells were purchased from Cell Bank of Chinese Academy of Sciences and cultured in DMEM (06-1055-57-1ACS, Biological Industries) with 10% fetal bovine serum (04-002-1A, Biological Industries), penicillin (100U/mL), streptomycin (10μg/mL) at 37^◦C with 5% CO₂ in a humidified incubator. Pirarubicin (S1393), necrostatin-1 (S8037) and SC79 (S7863) were purchased from Selleck. RIPK1 antibody (ab72139), P21 antibody (ab109520), β-actin antibody (ab8226) and GAPDH antibody (ab8245), Rb antibody (ab181616) and pRb^S780 antibody (ab173289) were purchased from abcam. AKT antibody (4691) and p-AKT^Ser473 antibody (9271) were purchased from Cell Signaling Technology. Goat anti-Mouse (A00160) and anti-Rabbit HRP (A00166) were purchased from GenScript.

Huang H., Chen T., Zhou Y., Geng L., Shen T., Zhou L, & Zheng S. (2018). RIPK1 Inhibition Enhances Pirarubicin Cytotoxic Efficacy through AKT-P21-dependent Pathway in Hepatocellular Carcinoma. International Journal of Medical Sciences, 15(14), 1648-1657.

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Inhibition of Tubulin and HO-1 Signaling

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MK-2206 (S1078) and SC79 (S7863) were from Selleck (Shanghai, China). The anti-β-tubulin antibody (SAB4500088) and the heme oxygenase-1 (HO-1) inhibitor Zinc protoporphyrin (ZnPP, 282820) were obtained from Sigma (St. Louis, MO). All other antibodies were from Cell Signaling Tech (Nanjing, China) and Santa Cruz Biotech (Shanghai, China) as described [6 (link), 10 (link)].

Gong Y.Q., Huang W., Li K.R., Liu Y.Y., Cao G.F., Cao C, & Jiang Q. (2016). SC79 protects retinal pigment epithelium cells from UV radiation via activating Akt-Nrf2 signaling. Oncotarget, 7(37), 60123-60132.

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