Oxidative stress markers were assessed using a malondialdehyde (MDA) Colorimetric assay kit: TBA method (Elabscience, Houston, Texas USA; E-BC-K025-M), and Glutathione Peroxidase (GSH-Px) Activity Assay Kit (Elabscience, Houston, Texas USA; E-BC-K096-M WST-1 Method). For PCR, TRIzol reagent (Merck; T9424), iso-propanol (Merck; B6916), cDNA using a high-capacity cDNA RT kit (Applied Biosystems; 4368814), Power Up SYBER Green Master Mix (Applied Biosystems; A25742; Austin, Texas, USA), Forward and Reverse Primer were procured from Inqaba Biotechnical industries (Hatfield, Pretoria, South Africa).
Mda colorimetric assay kit tba method
Manufactured by Elabscience
Sourced in United States
The MDA) Colorimetric assay kit: TBA method is a laboratory product designed to measure malondialdehyde (MDA) levels. MDA is a common biomarker used to assess oxidative stress. The kit utilizes the thiobarbituric acid (TBA) method, a widely used technique for MDA quantification. The core function of this product is to provide a colorimetric assay for the detection and quantification of MDA in various sample types.
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Lab products found in correlation
Powerup syber green master mix, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Merck Group (1 mentions)
Forward and reverse primer, by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Merck Group (1 mentions)
High capacity cdna rt kit, by Thermo Fisher Scientific (1 mentions)
E bc k025 m, by Elabscience (1 mentions)
Gsh colorimetric assay kit, by Elabscience (1 mentions)
Ferrous iron colorimetric assay kit, by Elabscience (1 mentions)
2 protocols using mda colorimetric assay kit tba method
1
Oxidative Stress Biomarker Assessment
2
Redox Status Evaluation in Aged Mouse Hippocampus
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Aged mice were decapitated under deep anesthesia, and the hippocampus was immediately collected. The fresh tissues of hippocampus were perfused with PBS containing heparin to remove blood and clots. After weighing the tissue, it was homogenized in slurry medium. The relative concentration of GSH was detected using GSH Colorimetric Assay Kit (Elabscience, Wuhan, China). The MDA content was detected using an MDA Colorimetric Assay Kit (TBA method) (Elabscience, Wuhan, China) and the determination of Fe2+ level using Ferrous Iron Colorimetric Assay Kit (Elabscience, Wuhan, China). All kits were used according to the manufacturer's instructions.
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