Donkey anti rabbit and sheep anti mouse horseradish peroxidase conjugated secondary antibodies

For in vitro experiments, protein was collected and subjected to immunoblot analyses as previously described (17 (link)). For in vivo experiments, GFP-positive human tumor tissue was dissected from the mouse brain and snap frozen in liquid nitrogen. Samples were homogenized (Polytron Brinkman) in lysis buffer (17 (link)) and incubated for 0.5 hour at 4°C; protein was subjected to immunoblot analyses as previous described (17 (link)). Anti-phospho-DNAPK (Ser2056; #ab14918) was obtained from Abcam. Anti-total-DNA-PK was obtained from Thermo-Fisher Scientific. Anti-b-actin and anti-Sox2 (human-specific, #D6D9) were obtained from Cell Signaling Technology. Donkey anti-rabbit and sheep anti-mouse horseradish peroxidase-conjugated secondary antibodies were purchased from GE Healthcare Life Sciences. Bands were quantified by densitometry analysis using Image Studio Lite (LICOR Biosciences).