Nitric oxide kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Nitric oxide (NO) kit is a laboratory equipment designed to measure the concentration of nitric oxide in various samples. It provides a reliable and accurate method for quantifying this important signaling molecule without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (3 mentions) Reactive oxygen species assay kit, by Beyotime (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Triton x 100, by Merck Group (1 mentions) Apomorphine, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Procaspase 3, by Cell Signaling Technology (1 mentions) Irak4, by Cell Signaling Technology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Prodium iodide pi, by Merck Group (1 mentions) Rabbit anti bcl 2 polyclonal antibody, by Cell Signaling Technology (1 mentions) Reverse transcription kit, by Promega (1 mentions) Total protein extraction kit, by Keygen Biotech (1 mentions) Superoxide dismutase sod kit, by Nanjing Jiancheng (1 mentions) Malondialdehyde mda kit, by Nanjing Jiancheng (1 mentions) Phosphatase inhibitor complex 3, by Sangon (1 mentions) Nuclear and cytoplasmic protein extraction kit, by Sangon (1 mentions) Glutathione gsh kit, by Nanjing Jiancheng (1 mentions) Tnf α, by Sangon (1 mentions)

Spelling variants of this product

Nitric oxide assay kit (55 citations) Nitric Oxide Synthase Assay Kit (5 citations) Nitric oxide (4 citations) Nitric Oxide (NO) assay kit (Nitrate reductase method; (2 citations) Nitric oxide Test Kit (2 citations) Total nitric oxide synthase assay kit (2 citations) A012 Nitric Oxide (NO) assay kit (2 citations) Nitric Oxide Colorimetric Assay Kit (2 citations)

14 protocols using nitric oxide kit

Extraction and Characterization of PLA

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Piper longum was purchased from Anguo, Hebei province, China, in 2014 and identified by Rong Luo, associate professor, School of Traditional Chinese Medicine, Capital Medical University. The voucher specimens of this material have been deposited in School of Traditional Chinese Medicine, Capital Medical University.
The PLA extract was made as our previous work described [15 (link)]. The content of total alkaloids was 74.6 % determined by UV, meanwhile the contents of piperine and piperlonguminine were 53.08 and 1.73 % respectively determined by HPLC. PLA was analyzed in a previous study carried out by our laboratory. Refer to this work for information about detailed compositions and chromatogram of PLA [15 (link)].
Lipopolysaccharide (LPS, from Escherichia coli. serotype O26:B6), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), mouse anti-tyrosine hydroxylase antibody, TritonX-100 and apomorphine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-Iba-1 antibody was purchased from BOSTER (Wuhan, China). Interleukin-1β (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-alpha (TNF-α) and reactive oxygen species (ROS) enzyme-linked immunosorbent assay (ELISA) kits were purchased from MultiScience Biotech Co., Ltd (Hangzhou, China). Nitric oxide (NO) kit was purchased from NanjingJiancheng Bioengineering Institute (Jiangsu, China).

He H., Guo W.W., Xu R.R., Chen X.Q., Zhang N., Wu X, & Wang X.M. (2016). Alkaloids from piper longum protect dopaminergic neurons against inflammation-mediated damage induced by intranigral injection of lipopolysaccharide. BMC Complementary and Alternative Medicine, 16, 412.

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Investigating Inflammatory Signaling Pathways

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Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Gibco-BRL (USA). Fetal bovine serum (FBS) was from Hyclone Lab Inc. (USA). LPS (Escherichia coli 0111:B4), sulforhodamine B (SRB), prodium iodide (PI) and CAPE were from Sigma Co. (USA). Primary antibodies against TLR4, NF-κB p65, β-actin and secondary antibody (horseradish peroxidase) were from Santa Cruz Biotechnology (USA). Primary antibodies against MyD88, TRIF, IRAK4, LC3B, PARP and procaspase 3 were purchased from Cell Signaling Technology (USA). Secondary antibody for immunofluorescence, donkey anti-rabbit IgG Alexa Fluor-488 was purchased from Life Technologies (USA). Nitric oxide (NO) kit was from Nanjing Jiancheng Bioengineering Institute (China). All other reagents were ultrapure grade.

Chang H., Wang Y., Yin X., Liu X, & Xuan H. (2017). Ethanol extract of propolis and its constituent caffeic acid phenethyl ester inhibit breast cancer cells proliferation in inflammatory microenvironment by inhibiting TLR4 signal pathway and inducing apoptosis and autophagy. BMC Complementary and Alternative Medicine, 17, 471.

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Oxidative Stress Biomarker Assays

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Total antioxidant capacity (TAC) kit (Cat. No: S0119), lipid peroxidation MDA assay kit (Cat. No: S0131), and total SOD assay kit (Cat. No: S0109) were purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). Nitric oxide (NO) kit (Cat. No: A012) was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). 8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA kit was obtained from Beijing Fangcheng Biotechnology Company (Beijing, China). Rabbit anti-Nox4 polyclonal antibody (Cat. No: NB110-58849) was obtained from Novus Biologicals (Littleton, CO, USA). Mouse anti-Cyto-C monoclonal antibody and mouse anti-p-IκBα monoclonal antibody (Cat. No: sc-8404) were from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against NF-κB-p65 (ab16502), NF-κB-pp65 (ab86299), IκBα (ab32518) were obtained from Abcam Biocompany (Cambridge, MA, USA). Rabbit anti-Bcl-2 polyclonal antibody was obtained from Cell signaling (Danvers, MA, USA). Estradiol valerate (17-beta estradiol) tablets were purchased from Bayer Chemical Company (Leverkusen, Germany). All other reagents, except specially identified, were from Sigma (St. Louis, MO, USA).

Wang L., Ma R., Guo Y., Sun J., Liu H., Zhu R., Liu C., Li J., Li L., Chen B., Sun L., Tang J., Zhao D., Mo F., Niu J., Jiang G., Fu M., Brömme D., Zhang D, & Gao S. (2017). Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway. Frontiers in Pharmacology, 8, 266.

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Oxidative Stress Biomarkers Analysis

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Malondialdehyde (MDA) kit (Batch number: A003-2), nitric oxide (NO) kit (Batch number: A012) superoxide dismutase (SOD) kit (Batch number: A001-1) and glutathione (GSH) kit (Batch number: A006) were purchased from Nanjing Jiancheng Bioengineering Co., Limited; Total protein extraction kit (Batch number: KGP2100) was purchased from Nanjing KeyGen Biotech Co., Limited; Nuclear and cytoplasmic protein extraction kit (Batch number: BB-3112-1), phosphatase inhibitor complex III (Batch number: PL019-1), modified bicinchoninic acid protein assay kit (Batch number: SK3051) were purchased from Shanghai Sangon Biotech Co., Limited; Animal tissue total ribonucleic acid (RNA) extraction kit (Batch number: Lo423) was purchased from Beijing TianGen Biotech Co., Limited; Reverse transcription kit (Batch number: ADA3500) was purchased from Promega; Rabbit antimouse β-actin antibody (Batch number: sc-130656), HO-1 antibody (Batch number: sc-10789), Nrf2 antibody (Batch number: sc-722) were purchased from Santa Gruz Biotech Co., Limited.

Huang X.P., Qiu Y.Y., Wang B., Ding H., Tang Y.H., Zeng R, & Deng C.Q. (2014). Effects of Astragaloside IV combined with the active components of Panax notoginseng on oxidative stress injury and nuclear factor-erythroid 2-related factor 2/heme oxygenase-1 signaling pathway after cerebral ischemia-reperfusion in mice. Pharmacognosy Magazine, 10(40), 402-409.

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Isolation and Identification of Lactobacillus paracasei GL1

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The strain of GL1 was isolated by the traditional streaking separation method from a traditional region of Tibet in Naqu, China, and it was recognized by 16S rRNA sequence analysis. A fragment 16S rDNA of GL1 was amplified by the 16S rDNA primer (Supplementary Table S1). The amplification was performed in 50 μL (final volume) of the reaction mixture containing 25 μL Rapid Taq Master Mix, 2 uL of each primer, 8 to 10 ng of purified genomic DNA 2 μL, and 19 μL deionized water. The PCR parameters were 94 °C for 2 min, 30 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 90 s, and a final extension at 72 °C for 10 min. Then, the sequences were determined by the BLAST program of the GenBank database, and GL1 was identified as L. paracasei. The RAW264.7 murine macrophage cell line was purchased from Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. MD34 dialysis bags were purchased from Biosharp (Beijing Labgic Technology Co. Ltd., Beijing, China). The nitric oxide (NO) kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The primers of TNF-α, IL-1β, iNOS, and ꞵ-actin were synthesized by the Sangon Bioengineering Institute (Shanghai, China).

Wang X., Tian J., Zhang X., Tang N., Rui X., Zhang Q., Dong M, & Li W. (2022). Characterization and Immunological Activity of Exopolysaccharide from Lacticaseibacillus paracasei GL1 Isolated from Tibetan Kefir Grains. Foods, 11(21), 3330.

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Inflammatory Response Evaluation in RAW 264.7 Cells

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RAW 264.7 cells were purchased from the Cell Bank at the China Academy of Science (Shanghai, China). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), phosphate-buffered saline, penicillin, and streptomycin were purchased from Gibco (New York, United States). Dimethyl sulfoxide (DMSO) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sangon Biotech (Shanghai, China). Lipopolysaccharides (LPS) and indomethacin were obtained from Sigma-Aldrich (United States). The nitric oxide (NO) kit was purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were obtained from Beijing Solarbio Science & Technology Co. Ltd (China). Ultrapure water was obtained from Gen Pure (Thermo Fisher Scientific, Waltham, MA, United States). Acetonitrile (HPLC grade) was purchased from Thermo Fisher Scientific. All reagents were of analytical grade. Reference compounds for the 15 analytes were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Purified water was obtained using a Milli-Q system (Millipore, Burlington, MA, United States).

Ji M., Wang C., Yang T., Meng X., Wang X, & Li M. (2021). Integrated Phytochemical Analysis Based on UPLC–MS/MS and Network Pharmacology Approaches to Explore the Effect of Odontites vulgaris Moench on Rheumatoid Arthritis. Frontiers in Pharmacology, 12, 707687.

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Vascular Inflammation Evaluation Protocol

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KGM was purchased from Hubei Xiangfan Huipu Biochemical Science and Technology Engineering Co., Ltd. A cholesterol kit was purchased from Beckman Reagents, Inc. An endothelin kit was purchased from Tianjin Chiye Biological Products Co., Ltd. The nitric oxide kit was purchased from the Nanjing Jiancheng Institute of Biological Engineering. The rabbit anti-VCAM-1 polyclonal antibody was purchased from Wuhan Bode Biological Engineering Co., Ltd. An S–P immunohistochemical kit was purchased from Wuhan Bode Biological Engineering Co., Ltd. A DAB color kit was purchased from Wuhan Bode Biological Engineering Co., Ltd. LY294002 was purchased from Med Chem Express, Company.

Weng J., Chen M., Shi B., Liu D., Weng S, & Guo R. (2023). Konjac glucomannan defends against high-fat diet-induced atherosclerosis in rabbits by promoting the PI3K/Akt pathway. Heliyon, 9(2), e13682.

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Spectroscopic and Chromatographic Techniques

Cited 1 time

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The following technologies and materials were used in this research: UV spectra (Shimadzu, Kyoto, Japan); IR spectra (Bruker Vector 22 spectrophotometer, Bruker Optics GmbH, Ettlingen, Germany); Mass spectra (API QSTAR time-of-flight spectrometer, MDS Sciqaszex, Concord, Ontario, Canada). NMR spectra (Bruker AM-400, Bremerhaven, Germany); silica gel (200–300 and 300–400 mesh, Qingdao Marine Chemical Inc., China); Rp-18 gel (40–63 μm, Merck, Darmstadt, Germany); Sephadex LH-20 (20–150 μm, Amersham Biosciences, Uppsala, Sweden); YMC*GEL ODS-A-HG (50 μm, YMC Co. Ltd., Kyoto, Japan) (34 (link)).
The following reagents were used in this research: FBS (Gibco, Grand Island, NE, USA); DMEM, neutral red (Solarbio, Beijing, China); Nitric oxide kit (Nanjing Jiancheng Bioengineering Institute); IL-6 and TNF-α ELISA kit (Beijing 4A Biotech Co., Ltd., Beijing, China); Primer iNOS, TNF-α, IL-6 and COX-2 (Thermo Fisher Scientific, Shanghai, China); Reactive Oxygen Species Assay Kit (Beyotime Biotechnology, Shanghai, China); PrimeScriptTMRT reagent kit with gDNA Eraser kit and TB Green TM Ex TaqTM II (Tli RNadeH Plus, Accurate Biology, Hunan, China), Bulk kit (TaKaRa, Accurate Biology, Hunan, China); Antibody NF-κB p65, phospho-NF-κB p65, iNOS, COX-2, IκBα, and phospho-IκBα (Cell Signaling, Beverly, MA, USA); LPS (Sigma-Aldrich, St. Louis, MO, USA) (35 (link)).

Niu Y., Wang B., Zhou L., Ma C., Waterhouse G.I., Liu Z., Ahmed A.F., Sun-Waterhouse D, & Kang W. (2021). Nigella sativa: A Dietary Supplement as an Immune-Modulator on the Basis of Bioactive Components. Frontiers in Nutrition, 8, 722813.

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Exploring anti-inflammatory mechanisms in vitro

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Dulbecco’s Modified Eagle’s Medium (DMEM) and neutral red were purchased from Solarbio (Beijing, China). Fetal Bovine Serum (FBS) was purchased from Gibco (Grand Island, NY, USA). Nitric oxide kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TNF-α and IL-6 ELISA kit were obtained from Beijing 4A Biotech Co, Ltd (Beijing, China). Primer iNOS, TNF-α, IL-6, TLR4 and MyD88 were purchased from Thermo Fisher scientific (Shanghai, China). Reactive Oxygen Species Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China). PrimeScriptTMRT reagent kit with gDNA Eraser kit and TB Green TM Ex TaqTM II (Tli RNadeH Plus) and Bulk kit were purchased from TaKaRa. Antibody NF-κB p65, phospho-NF-κB p65, p38 MAPK, phospho-p38 MAPK, SAPK/JNK, phospho-SAPK/JNK, p44/p22 MAPK and phospho-p44/p22 MAPK were purchased from Cell Signaling (Beverly, MA, USA). NF-κB inhibitor (PDTC), ERK inhibitor (PD98059), JNK inhibitor (SP600125) and p38 inhibitor (SB203580) Lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA)

Wang H., Xu X., Yin Z., Wang M., Wang B., Ma C., Wang J, & Kang W. (2021). Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway. International Journal of Immunopathology and Pharmacology, 35, 20587384211010058.

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Rat Biomarker Quantification Protocol

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Blood samples were collected from inferior vena cava by open surgery under inhalational anaesthesia at each indicated time point, as described before, after which the rats were sacrificed due to massive blood loss, and tissue samples were collected. The serum levels of prostacyclin I2 (PG I₂) (Rat Prostaglandin E2 ELISA Kit, Cusabio, Wuhan, China), prostaglandin E2 (PG E₂) (Rat Prostacycline ELISA Kit, Cusabio, Wuhan, China), nitric oxide (NO) (Nitric Oxide Kit, Jiancheng Bioengineering, Nanjing, China), ghrelin (ELISA Kit For Ghrelin, USCN Kit, Wuhan, China), tumour necrosis factor-alpha (TNF-α) (Rat TNF-α ELISA Kit, Thermo Fisher Scientific), interleukin 1β (IL-1β) (Rat IL-1β ELISA Kit, Multi Sciences Biotech, Hangzhou, China), interleukin-6 (IL-6) (Rat IL-6 ELISA Kit, Multi Sciences Biotech, Hangzhou, China), and interleukin-10 (IL-10) (Rat IL-10 ELISA Kit, Multi Sciences Biotech, Hangzhou, China) were determined using commercially available ELISA kits following the manufacturer’s instructions.

Zhang Y., Han X., Li Z., Zhang Y., Liang L., Ma X., Liu H., Gao Y., Li Q., Chen X., Lv Y, & Ren F. (2021). Physiological and histopathological effects of electroporation pulse on stomach of rats. BMC Gastroenterology, 21, 351.

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