Vertex 70 ftir ft raman spectrometer

The treated dentin was analyzed by FT-Raman. An FT-Raman spectrometer (Bruker VERTEX 70 FTIR/ FT-Raman spectrometer, Bruker Optics Inc., Ettlingen, Karlsruhe, Germany) with a liquid nitrogen-cooled Ge detector was used to collect the data. To excite the spectra, an air-cooled Nd: YAG laser (λ ¼ 1064.1 nm) source was used. The power of the Nd: YAG laser incident was 100 mW at a spectral resolution of 4 cm -1 . The Raman system was calibrated with a silicon semiconductor using a Raman peak of 521 cm -1 . The spectrum of the specimen was obtained and then analyzed by selecting a range from 400 to 4000 cm -1 . Distribution of the organic components was examined using the peak area of the CH stretch band between 2940 and 2880 cm -1 , and this band was used for image analysis. A qualitative spectral analysis of the changes in organic content was performed. The spectra in the region of interest, between 2880 and 2940 cm -1 , were analyzed with analytical software (Microcal Origin ® 5.0 Software, Inc., Westborough, USA). The CH stretching mode (2880 and 2940 cm -1 ) was used in this study to detect the collagen component as a signature of organic content in dentin samples. 18 (link)