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25 gram solid phase cartridge

Manufactured by Teledyne
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The 25 gram solid phase cartridge is a laboratory equipment designed for sample preparation and purification. It provides a platform for solid-phase extraction, filtration, and concentration of various analytes from complex matrices. The core function of this cartridge is to facilitate efficient and reproducible sample handling and processing for analytical and research applications.

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Lab products found in correlation

Celite 545, by Thermo Fisher Scientific (2 mentions) Celite, by Teledyne (2 mentions)

2 protocols using 25 gram solid phase cartridge

1

Large-scale extraction and purification of fungal compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
A. fumigatus strains were inoculated (1.0×106 spores mL−1) into 1 L GMM43 (link) in a 2 L Erlenmeyer flask at 37 °C with shaking at 220 rpm. After 3.5 days, liquid fungal cultures including fungal tissue and media were frozen using a dry ice acetone bath, and lyophilized. The lyophilized residues were extracted with 500 mL of MeOH for 1 h with vigorous stirring. Extracts were filtered over cotton and evaporated on Celite-545 (Acros Organics) to dryness. The dry Celite was then loaded into a 25 gram solid phase cartridge (Isco) and subjected to large-scale reverse phase chromatography (see Chromatographic enrichment section below for details). Compounds 4 and 5 are also minor components of the culture media.
Baccile J.A., Spraker J.E., Le H.H., Brandenburger E., Gomez C., Bok J.W., Macheleidt J., Brakhage A.A., Hoffmeister D., Keller N.P, & Schroeder F.C. (2016). Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus. Nature chemical biology, 12(6), 419-424.
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2

Large-scale extraction and purification of fungal compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
A. fumigatus strains were inoculated (1.0×106 spores mL−1) into 1 L GMM43 (link) in a 2 L Erlenmeyer flask at 37 °C with shaking at 220 rpm. After 3.5 days, liquid fungal cultures including fungal tissue and media were frozen using a dry ice acetone bath, and lyophilized. The lyophilized residues were extracted with 500 mL of MeOH for 1 h with vigorous stirring. Extracts were filtered over cotton and evaporated on Celite-545 (Acros Organics) to dryness. The dry Celite was then loaded into a 25 gram solid phase cartridge (Isco) and subjected to large-scale reverse phase chromatography (see Chromatographic enrichment section below for details). Compounds 4 and 5 are also minor components of the culture media.
Baccile J.A., Spraker J.E., Le H.H., Brandenburger E., Gomez C., Bok J.W., Macheleidt J., Brakhage A.A., Hoffmeister D., Keller N.P, & Schroeder F.C. (2016). Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus. Nature chemical biology, 12(6), 419-424.
+ Open protocol
+ Expand

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