Mouse anti human α tubulin clone dm1a monoclonal antibody

Manufactured by Cell Signaling Technology

The Mouse anti-human α-tubulin (clone DM1A) monoclonal antibody is a laboratory reagent used to detect and study the alpha-tubulin protein, a key component of the cytoskeleton in eukaryotic cells. This antibody specifically recognizes the human alpha-tubulin protein.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti human mcl 1 monoclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human parp polyclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human npm polyclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human p npm thr199 polyclonal antibody, by Cell Signaling Technology (1 mentions)

2 protocols using mouse anti human α tubulin clone dm1a monoclonal antibody

Apoptotic Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: rabbit anti-human BCL-2 (clone D55G8) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human BCL-X_L (clone 54H6) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human MCL-1 monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human BCL-W (Clone 31H4) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human Noxa (clone EPR9735B) monoclonal antibody (1:1,000, Abcam); rabbit anti-human BIM (1:1,000, Cell Signaling Technology); mouse anti-human cytochrome c (clone 7H8.2C12) monoclonal antibody (1:1,000 BD pharmingen); rabbit anti-human PARP (Clone 9542S) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human cleaved caspase 3 (Clone 5AE17) monoclonal antibody (1:1000, Cell Signaling Technology); mouse anti-human α-tubulin (clone DM1A) monoclonal antibody (1:10,000, Cell Signaling Technology) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit (clone NA9340V) and goat anti-mouse (clone NXA931) secondary antibodies (1:10,000 GE Healthcare). See Supplementary Materials and Methods for detailed protocol.

Bate-Eya L.T., den Hartog I.J., van der Ploeg I., Schild L., Koster J., Santo E.E., Westerhout E.M., Versteeg R., Caron H.N., Molenaar J.J, & Dolman M.E. (2016). High efficacy of the BCL-2 inhibitor ABT199 (venetoclax) in BCL-2 high-expressing neuroblastoma cell lines and xenografts and rational for combination with MCL-1 inhibition. Oncotarget, 7(19), 27946-27958.

+ Open protocol

+ Expand

Western blot analysis of cell signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: mouse anti-human Rb (clone G3-245) monoclonal antibody (1:1000, BD Biosciences); rabbit anti-human p-Rb (Thr⁸²¹) polyclonal antibody (1:1000, BioSource International); mouse anti-human MYCN (clone B8.4.B) monoclonal antibody (1:5000, BD Biosciences); rabbit anti-human PARP polyclonal antibody (1:2000, Cell Signaling Technology); rabbit anti-human NPM polyclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-human p-NPM (Thr199) polyclonal antibody (1:500, Cell Signaling Technology); rabbit anti-human β-actin (clone 13E5) monoclonal antibody (1:1000, Cell Signaling Technology); mouse anti-human β-actin (clone AC-15) monoclonal antibody (1:20000, Abcam); mouse anti-human α-tubulin (clone DM1A) monoclonal antibody (1:1000, Cell Signaling Technology) and IRDye 800CW goat anti-rabbit and goat anti-mouse secondary antibodies (1:5000, Li-COR). See Supplementary Materials and Methods for a detailed protocol.

Dolman M.E., Poon E., Ebus M.E., den Hartog I.J., van Noesel C.J., Jamin Y., Hallsworth A., Robinson S.P., Petrie K., Sparidans R.W., Kok R.J., Versteeg R., Caron H.N., Chesler L, & Molenaar J.J. (2015). Cyclin-dependent kinase inhibitor AT7519 as a potential drug for MYCN-dependent neuroblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(22), 5100-5109.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!