N n diisopropylcarbodiimide dic trifluoroacetic acid tfa scavenger triisopropylsilane tis

The solid phase carrier Fmoc-Rink Amide MBHA resin, the specifically protected amino acids needed for target structure synthesis Fmoc-L/D-Phe-OH, Fmoc-L/D-Tyr(OBu^t)-OH, Fmoc-Thr(OBu^t)-OH, Fmoc-Lys(Boc)-OH and Fmoc-D-Trp(Boc)-OH, activation agents N,N,N’,N’-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU) and N,N’-Diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), scavenger triisopropylsilane (TIS) and base N,N-Diisopropylethylamine (DIPEA) were purchased from Iris Biotech (Wunsiedel, Germany). The solvents N,N’-dimetylformamide (DMF) and dichloromethane (DCM) were obtained from Valerus (Sofia, Bulgaria), and 4-N,N-dimethylaminopyridine (DMAP) was obtained from Sigma-Aldrich (Ansbach, Germany). All reagents and solvents were used as purchased without any additional purification or pretreatment.

All specifically protected amino acids Fmoc-L-Phe-OH, Fmoc-L-Glu(tBu)-OH, Fmoc-L-Leu-OH, Fmoc-L-Nle-OH, Fmoc-L-Ile-OH, and Fmoc-L-Val-OH, as well as Fmoc-Rink Amide MBHA and 2-Chlorotrityl chloride resin (2-CTC), activation agents N,N,N′,N′-tetramethyl-O-(1Hbenzotriazol-1-yl)uronium hexafluorophosphate (HBTU) and N,N′-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), scavenger triisopropylsilane (TIS), and base N,N-diisopropylethylamine (DIPEA) were purchased from Iris Biotech (Wunsiedel, Germany). The solvents N,N′-dimetylformamide (DMF) and dicholomethane (DCM) are obtained from Valerus (Sofia, Bulgaria), and 4-N,N-dimethylaminopyridine (DMAP) is from Sigma-Aldrich (Ansbach, Germany). All reagents and solvents were used without any preliminary treatment.