Immunohistochemistry for NLRP3 and gasdermin E (GSDME) was conducted, the tissues sections were blocked with 8% goat serum in PBS, and then incubated with anti-NLRP3 antibody (Abcam, ab214185, dilution 1:100) and anti-GSDME antibody (Abcam, ab230482, dilution 1:500) at 4 ˚C for 12 h. Thereafter, the sections were incubated with anti-rabbit IgG H&L (HRP) (Abcam, ab6721, dilution 1:1000) at room temperature for 1 h. Finally, the sections were examined under a light microscope and Nikon Photo-Imaging system (H550L, Tokyo, Japan).
Photo imaging system h550l
Manufactured by Nikon
Sourced in Japan
The Nikon Photo-Imaging system (H550L) is a lab equipment product designed for photographic imaging applications. It provides a stable and reliable platform for capturing high-quality images in a controlled laboratory environment. The system's core function is to facilitate the process of image acquisition and analysis, allowing users to capture, store, and process visual data for their specific research or industrial needs.
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Lab products found in correlation
3 protocols using photo imaging system h550l
1
NLRP3 and GSDME Immunohistochemistry
2
GPX4 Immunohistochemical Localization
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Immunohistochemistry for glutathione peroxidase 4 (GPX4) was conducted. The sections were blocked with 8% goat serum in PBS and then incubated with anti‐GPX4 antibody (1:1000; Santa Cruz, Cat#: sc‐166,570) at 4°C for 12 h. Thereafter, the sections were incubated with anti‐mouse HRP reagent (Sigma‐Aldrich, Cat#: A‐9044) at room temperature for 1 h. Finally, the sections were examined under a light microscope and Nikon Photo‐Imaging system (H550L, Tokyo, Japan).
3
Evaluating Cardiac Tissue Morphology
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The harvested heart was fixed in 4% paraformaldehyde for 24 h, subsequently dehydrated in ethanol and xylene series, and finally embedded in paraffin. To evaluate the cross-sectional sizes of cardiomyocytes and cardiac interstitial fibrosis, 5-μm cardiac tissue slides were stained with hematoxylin and eosin (H&E) and picrosirius red (PSR). Subsequently, images of the slides were obtained by optical microscopy with a Nikon Photo-Imaging System (H550L, Tokyo, Japan). For cardiomyocytes sizes and interstitial fibrosis quantification, images were analyzed with the Image-pro Plus 6.0 software (Maryland, USA).
For immunohistochemistry of myocardial sFRP2 expression, the cardiac tissue sections were deparaffinized, blocked with 8% goat serum, and then incubated with 1:100 diluted anti-sFRP2 antibody overnight at 4 °C. After washing, the slides were incubated for 1 h with anti-rabbit HRP reagent (Gene Tech, Shanghai, China) at 37 °C, rinsed, and developed with a peroxide-based substrate DAB kit for 5 min (Gene Tech, Shanghai, China). Finally, images of the slides were obtained by light microscopy with a Nikon Photo-Imaging System (H550L, Tokyo, Japan).
For immunohistochemistry of myocardial sFRP2 expression, the cardiac tissue sections were deparaffinized, blocked with 8% goat serum, and then incubated with 1:100 diluted anti-sFRP2 antibody overnight at 4 °C. After washing, the slides were incubated for 1 h with anti-rabbit HRP reagent (Gene Tech, Shanghai, China) at 37 °C, rinsed, and developed with a peroxide-based substrate DAB kit for 5 min (Gene Tech, Shanghai, China). Finally, images of the slides were obtained by light microscopy with a Nikon Photo-Imaging System (H550L, Tokyo, Japan).
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