Evaluation of in vivo Hb-degradation was performed by analyzing the presence of free heme using the Heme Colorimetric Assay Kit (BioVision, Milpitas, CA, USA) in plasma of animals subjected to varied oxygen environments.
Heme colorimetric assay kit
Manufactured by Abcam
Sourced in United States
The Heme Colorimetric Assay Kit is a laboratory reagent designed to quantify the amount of heme in samples. The kit utilizes a colorimetric method to measure the concentration of heme, providing a simple and efficient way to analyze heme levels in various biological samples.
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Lab products found in correlation
2 protocols using heme colorimetric assay kit
1
In vivo Hb-degradation Analysis
2
Quantifying Intracellular Heme Levels
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Intracellular heme levels were measured using a heme colorimetric assay kit (BioVision, Mountain View, CA, United States). Briefly, animal tissues were lysed in lysis buffer and homogenized using sonication. Aliquots (50 μL) from each diluted sample or standard were transferred into 96-well plates prior to the addition of 50 μL of heme assay kit solution to each well. After incubation for 30 min, the absorbance was measured at 570 nm using an automated microplate luminometer (BioTek, San Diego, CA, United States).
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