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Magmax stabilized blood tube rna isolation kit

Manufactured by Thermo Fisher Scientific

The MagMAX Stabilized Blood Tube RNA Isolation Kit is a laboratory equipment product designed for the isolation of RNA from stabilized blood samples. The kit utilizes magnetic bead-based technology to efficiently extract and purify RNA from blood collected in specialized tubes.

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2 protocols using magmax stabilized blood tube rna isolation kit

1

PAXgene Blood RNA Extraction and Quantification

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The PAXgene™ Blood RNA tubes were processed at the Genetic and Molecular Epidemiology Laboratory of PHRI and McMaster University, Hamilton ON. Paired samples were processed using the same RNA extraction and amplification method. Total RNA was isolated from samples using the QIAsymphony PAXgene Blood RNA Kit (QIAGEN) or the MagMAX Stabilized Blood Tube RNA Isolation Kit (LifeTech). RNA was then quantified with RiboGreen® (LifeTech) and Nanodrop (Nanodrop).
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2

Illumina Transcriptome Profiling Protocol

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Total RNA was isolated from the discovery cohort using the QIAsymphony PAXgene Blood RNA kit (Qiagen) according to the manufacturer's protocol. RNA was isolated from the validation cohort using the MagMAX Stabilized Blood Tube RNA Isolation kit (LifeTech). RNA quality was assessed with Nanodrop2000 (Nanodrop) and 2100 Bioanalyzer (Agilent), then quantified using Quant-IT RiboGreen (LifeTech). Total RNA was amplified and biotinylated using the Illumina TotalPrep RNA Amplification Kit (LifeTech). Samples were then hybridized to Illumina HumanRef-8v4 BeadChips (Illumina) and scanned on the iScan System (Illumina) as per manufacturer protocol.
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