Scurfy mice

WT C57BL/6, Mx1-Cre, Rosa/eYFP, Foxp3^YFP-Cre and Scurfy mice were obtained from Jackson Laboratories (Bar Harbor, ME). Congenic C57BL/6 (CD45.1), IFNAR KO, and RAG1 KO mice were obtained from Taconic Farms (Germantown, NY). IFNAR^fl/fl mice were kindly provided by (Ulrich Kalinke, Paul-Ehrlich Institut, Langen, Germany) and crossed to Foxp3^YFP-Cre. All other mice were bred in house. The Animal Care and Use Committee (ACUC) of the National Institute of Allergy and Infectious Diseases (NIAID) approved all experiments.

Wild type (WT) C57BL/6 (CD45.2), WT C57BL/6 (CD45.1), and RAG^−/− mice were obtained from Taconic Farms (Germantown, NY). Eos^−/− mice were bred in house. Eos^−/− mice were generated by deleting the last three exons of the gene (Supplemental Fig. 1A). Deletion of the last coding exon from other Ikaros family paralogues has resulted in a functional null allele and mice homozygous for this Eos^−/− allele lack expression of Eos mRNA and protein. Scurfy mice were obtained from Jackson Laboratories (Bar Harbor, ME). Eos^−/− Foxp3-GFP mice were generated in house by breeding to Foxp3^EGFP mice (Jackson Stock No:006769). The Animal Care and Use Committee of the National Institute of Allergy and Infectious Diseases (NIAID) approved all experiments.