Amino acid free dmem

Dulbecco’s modified Eagle medium (DMEM), Dulbecco’s phosphate buffered saline (DPBS) and penicillin–streptomycin–glutamine (100×) were obtained from Thermo Fisher (Waltham, MA, USA). Fetal bovine serum (FBS) was obtained from ICN Biomedicals (Costa Mesa, CA, USA). HEPES buffered saline solution was purchased from Lonza Ltd. (Walkersville, MD, USA). Trypsin-EDTA solution and L-cystine dihydrochloride were purchased from Sigma Chemical Company (St. Louis, MO, USA). Amino-acid-free DMEM was purchased from Nacalai Tesque (Kyoto, Japan). Sodium hydrogen carbonate (NaHCO₃), hydrogen peroxide (H₂O₂), 100 w/v% trichloroacetic acid solution (TCA), and 1 mol/L hydrochloric acid (HCl) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Reduced glutathione (GSH) and dichloromethane were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).

Dulbecco’s Modified Eagle Medium (DMEM), Dulbecco’s phosphate buffered saline (DPBS), and penicillin–streptomycin-glutamine (100 ×) were obtained from Thermo Fisher (Waltham, MA, USA). Fetal bovine serum (FBS) was obtained from ICN Biomedicals (Costa Mesa, CA, USA). HEPES buffered saline solution was purchased from Lonza Ltd. (Walkersville, MD, USA). Trypsin–EDTA solution and L-cystine dihydrochloride were purchased from Sigma Chemical Company (St. Louis, MO, USA). Amino acid-free DMEM was purchased from Nacalai Tesque (Kyoto, Japan). Sodium hydrogen carbonate (NaHCO₃), hydrogen peroxide (H₂O₂), 100 w/v% trichloroacetic acid solution (TCA), and 1 mol/L hydrochloric acid (HCl) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Reduced glutathione (GSH) and dichloromethane were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).