pH titrations of LS1000, LS1001, LS1006, and LS1107 were each conducted by diluting the respective compound from a concentrated DMSO stock solution into 15 mL of 0.1 M NaCl aqueous solution. The initial pH was lowered to ~4 with the addition of 0.1 M HCl. The pH was gradually increased with the addition of dilute NaOH to ~8.5. At approximately 14 points across this range, the pH was determined by an Accumet AB15 Basic pH meter (Fisher Scientific, Waltham, MA) and the absorption spectrum was obtained at each point.
Accumet basic ab15 ph meter
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Accumet Basic AB15 pH meter is a laboratory instrument designed to measure the pH of aqueous solutions. It features a large, easy-to-read LCD display and provides accurate pH measurements. The AB15 pH meter is a versatile and reliable tool for various applications that require precise pH monitoring and analysis.
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6 protocols using accumet basic ab15 ph meter
1
pH Titration of LS Compounds
2
Measuring pH of Anesthetic Mixtures
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The default pH values of ropivacaine (0.1% and 0.2%), dexamethasone, and contrast media were measured using an electronic pH probe (Fisher Scientific Accumet AB15 Basic pH Meter, Pittsburgh, PA, USA). The pH measurement of each mixture was additionally analyzed 5 times each at room temperature (26 °C) and body temperature (36 °C), and the value of the measurements was averaged. After mixing each solution, it was observed 10 min later, and the average value obtained from five repeated measurements was used.
3
Determining pH-Dependent Enzyme Kinetics
4
Synthesis of Radiolabeled Compounds
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Example 1
Kryptofix® 222 (K222) (>98.0%) was purchased from TCI chemicals. Anhydrous CH3CN (99.9+%, extra dry, acroseal) was purchased from Acros organics. All other chemicals and reagents were purchased from Millipore Sigma and used without further purification. Buffer pH was measured on a Fisher Scientific Accumet basic ab 15 pH meter. Thermoshaking was performed on an Eppendorf Thermomixer R. TLC plates were purchased from Millipore (TLC Silica gel 60 F254). RadioTLCs were read on a Bioscan AR-2000 plate reader.
5
Conjugated Whey Protein Probiotic Solution
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The conjugated WPH-MD solution was prepared according to the protocol described by [11 (link)]. Briefly, the WPH10 and maltodextrin (DE10) (Sigma Aldrich, St. Louis, MO, USA; Fisher Scientific, Waltham, MA, USA) were mixed at a concentration of 5% w/v in two 1 L sterile bottles of distilled water at room temperature. The mixture was then solubilized at 25 °C for 2 h using magnetic stirrers (Corning, model number PC-420 D, Abington, MA, USA). The mixture’s pH (accumet basic AB 15 pH meter, Fisher Scientific, USA) was then adjusted to 8.2 using 0.5 N KOH. Next, the solution was hydrated at 4 °C for 18 h and heated at 90 °C for 24 h. The pH was again adjusted at 8.2 after the hydration step and the pH was recorded at 0, 3, 5, and 8 h intervals of heating. The conjugated solution was immediately cooled in iced water after heating. Then, the solution was inoculated with the probiotic (BB12 and LA5) cell suspensions. Both the cultures were spiked in a ratio of 1:1. The conjugated whey protein probiotic solution was then refrigerated at 4 °C until spray-drying.
6
Characterizing Soil pH and Carbon-Nitrogen
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Soil pH was measured in water (1:1) and in NaF (Accumet Basic AB15 pH meter, Fisher Scientific Inc., Beverly, MA, USA). Soil pH in NaF was obtained to assess the degree of andic soil mineral weathering and its potential impact on hydroxide ion release [58] . NaF pH was measured after mixing 1 g soil with 50 mL NaF solution (1 M), continuously stirred for 2 min. Soil total C and N were determined using a LECO TruSpec CN analyzer (LECO Corp., St. Joseph, MI, USA) after samples were oven-dried at 60 °C and ground to pass a 60-mesh screen.
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