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Tryptone yeast extract agar

Manufactured by HiMedia
Sourced in India

Tryptone yeast extract agar is a nutrient-rich culture medium used for the growth and cultivation of a wide variety of microorganisms, including bacteria and fungi. It provides the necessary nutrients and growth factors for the optimal development of microbial colonies in a laboratory setting.

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2 protocols using tryptone yeast extract agar

1

Isolation and Analysis of Phytochemicals

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Plant material (fresh leaves) was collected from the G. B Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal Almora (HQ), Uttarakhand (79° 37.405ʺE; 29° 38.405ʺN; 1243 amsl), cleaned with distilled water, surface dried and finally stored at 4 °C. Standard compounds GA and BB were purchased from Sigma (India), N–N-dimethyformide (DMF) from SRL, N,O-bistriflouroacetamide: Trimethylchlorosilane (BSTFA:TMCS; 99:1; lot LB92699) from Supelco (India), methanol from Renkam (India), Tryptone yeast extract agar (TYA) and Potato dextrose agar (PDA) from Hi-media (India).
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2

Characterizing ACTK2 Strain Morphology

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The cultural characteristic of pure isolate was studied after incubation of strain ACTK2 for 7-14 days at 28˚C using various media: Tryptone Yeast Extract Agar (ISP-1), Yeast Extract-Malt Extract Agar (ISP-2), Oat Meal Agar (ISP-3), Starch-Casein nitrate Agar medium (ISP-4), Glycerol-Asparagine Agar (ISP-5), Peptone Yeast Extract Iron Agar (ISP-6), Modified ISP-2 (MISP-2), Starch Agar, Potato Dextrose Agar, Czapek Dox Agar, Nutrient Agar, Modified Nutrient Glucose Agar (MNGA) and Mueller Hinton Agar, procured from Hi-Media, Mumbai, India. Morphological parameters such as aerial and substrate mycelium, arrangements of spores and Gram staining reaction of the ACTK2 strain (16 ) were carried out as described in Bergy's Manual of Systematic Bacteriology and by Shirling and Gottlieb (17 , 18 (link)).
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