Cells and tissues were washed with cold PBS on ice and treated with RIPA lysis buffer (Millipore, Temecular, CA) containing a cOmplete protease inhibitor cocktail tablet (Roche, Madison, MI) and a PhosSTOP phosphotase inhibitor cocktail tablet (Roche, Madison, MI). The protein amount was determined using the Bio-Rad protein assay (Bio-Rad). After an equal amount of protein was loaded in each lane, they were separated by 10% (w/v) SDS-PAGE and then transferred to an Immobilon-P PVDF membrane (Millipore). Target proteins were immunodetected using specific primary antibodies and the horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, CA) was used as a secondary antibody. Positive bands were detected using the Supersignal Westpico Chemiluminescent Substrate (Thermo Scientific, Pittsburgh, PA). Antibodies for p-TAK1, TAK, p-IKK α/β, IkB-α, p-JNK, JNK and AKT were obtained from Cell Signaling Technology (Boston, MA), antibodies for p-AKT and HSP90α/β were from Santa Cruz Biotechnology (Santa Cruz, CA) and the antibody for GPR120 was from Novus Biologicals (Littleton, CO).
Antibody for gpr120
Manufactured by Novus Biologicals
Sourced in Canada
GPR120 Antibody is a laboratory reagent used to detect and study the G protein-coupled receptor 120 (GPR120) protein. GPR120 is involved in various physiological processes, including fatty acid sensing and regulation of insulin secretion. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to identify and analyze the expression of GPR120 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon p pvdf membrane, by Merck Group (2 mentions)
Phosstop phosphotase inhibitor cocktail tablet, by Roche (2 mentions)
Antibodies for p akt and hsp90α β, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated anti rabbit or anti mouse igg, by Jackson ImmunoResearch (2 mentions)
Ripa lysis buffer, by Merck Group (2 mentions)
P jnk, by Cell Signaling Technology (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Protein assay, by Bio-Rad (2 mentions)
Complete protease inhibitor cocktail tablet, by Roche (2 mentions)
2 protocols using antibody for gpr120
1
Western Blot Analysis of Signaling Proteins
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells and tissues were washed with cold PBS on ice and treated with RIPA lysis buffer (Millipore, Temecular, CA) containing a cOmplete protease inhibitor cocktail tablet (Roche, Madison, MI) and a PhosSTOP phosphotase inhibitor cocktail tablet (Roche, Madison, MI). The protein amount was determined using the Bio-Rad protein assay (Bio-Rad). After an equal amount of protein was loaded in each lane, they were separated by 10% (w/v) SDS-PAGE and then transferred to an Immobilon-P PVDF membrane (Millipore). Target proteins were immunodetected using specific primary antibodies and the horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, CA) was used as a secondary antibody. Positive bands were detected using the Supersignal Westpico Chemiluminescent Substrate (Thermo Scientific, Pittsburgh, PA). Antibodies for p-TAK1, TAK, p-IKK α/β, IkB-α, p-JNK, JNK and AKT were obtained from Cell Signaling Technology (Boston, MA), antibodies for p-AKT and HSP90α/β were from Santa Cruz Biotechnology (Santa Cruz, CA) and the antibody for GPR120 was from Novus Biologicals (Littleton, CO).
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