The novel IAP inhibitor AZD5582 was provided by AstraZeneca (London, UK). Fludarabine and dexamethasone were obtained from Sigma-Aldrich (Gillingham, UK). Soluble recombinant human TRAIL was purchased from Enzo Life Sciences (Exeter, UK). Mouse monoclonal antibodies against XIAP (clone 48/hILP/XIAP) and cIAP-2 (clone F30-2285,) were obtained from BD Biosciences (Oxford, UK). Goat antibody to cIAP-1 was from R&D Systems (Minneapolis, MN). Rabbit antibodies against cFLIP, Bcl-2 and Bcl-xL were from Cell Signalling Technology (New England Biolabs, Herts, UK). Rabbit antibody to Mcl-1 was from Santa Cruz Biotechnology (Insight Biotechnology, Middlesex, UK). Mouse monoclonal antibody to β-actin (clone AC-74) was from Sigma-Aldrich. Other chemicals, unless otherwise stated, were also obtained from Sigma-Aldrich.
Azd5582
Manufactured by AstraZeneca
Sourced in United Kingdom
AZD5582 is an experimental lab equipment product developed by AstraZeneca. It is designed for use in scientific research and laboratory settings. The core function of AZD5582 is to facilitate the measurement and analysis of various biological and chemical compounds. Further details about the intended use or specific capabilities of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Fludarabine, by Merck Group (1 mentions)
Mouse monoclonal antibody to β actin clone ac 74, by Merck Group (1 mentions)
Soluble recombinant human trail, by Enzo Life Sciences (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Capan 1, by American Type Culture Collection (1 mentions)
Z vad, by Merck Group (1 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
Panc0813, by American Type Culture Collection (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Capan 2, by American Type Culture Collection (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Mg132, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
2 protocols using azd5582
1
Investigating IAP Inhibitor AZD5582 Effects
2
Evaluating Pancreatic Cancer Cell Lines
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Synthetic IAP antagonist, AZD5582 was supplied by AstraZeneca. Co., Ltd. (Maccelsfiled, Cheshire, UK). The human pancreatic cancer cell lines including BxPC-3, Miapaca-2, Panc-1, Panc0813, PL45, Capan-1, Capan-2 and AsPC-1 were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). All cells were cultured according to the ATCC's recommended guide lines. XIAP and cIAP1 null mouse embryonic fibroblast cells were kindly provided by Dr. Colin S. Duckett (University of Michigan, Ann Arbor, MI, USA) and Dr. Robert Korneluk (Ontario Cancer Institute, USA), respectively. Cycloheximide, a protein synthesis inhibitor, MG132, a proteasome inhibitor and z-VAD, a pan-caspase inhibitor, were provided by Sigma (St. Louis, MO, USA).
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